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SN/T 1196-2003 (SNT 1196-2003)

SN/T 1196-2003_English: PDF (SNT1196-2003)
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SN/T 1196-2003English439 Add to Cart 4 days Corn genetically modified qualitative PCR detection method SN/T 1196-2003 Obsolete SN/T 1196-2003

BASIC DATA
Standard ID SN/T 1196-2003 (SN/T1196-2003)
Description (Translated English) Corn genetically modified qualitative PCR detection method
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B22
Word Count Estimation 11,190
Date of Issue 2003-03-17
Date of Implementation 2003-09-01
Quoted Standard GB/T 6682; SN/T 1193; SN/T 1194; SN/T 1204
Drafting Organization Liaoning Entry-Exit Inspection and Quarantine of the PRC
Administrative Organization National Certification and Accreditation Administration Committee
Regulation (derived from) National Quality Inspection (2012) 777; industry standard filing Notice 2013 No. 4 (No. 160 overall)
Proposing organization National Certification and Accreditation Administration Committee
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary This standard specifies test genetically modified maize sampling, sample preparation, PCR detection methods. This standard screening test for qualitative detection of genetically modified maize. Identification of this standard applies to maize MON810, Bt11, Event176, T14/T25, CBH351, GA21 qualitative detection of genetically modified strains.

SN/T 1196-2003
Corn genetically modified qualitative PCR detection method
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Qualitative PCR of transgenic components in maize
Detection method
Released on.2003-03-17
2003-09-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Liaoning Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Cao Jijuan, Chen Mingsheng, Lu Xing'an, Zhao Wei, Wang Xiufen.
This standard is the industry standard for inspection and quarantine issued for the first time.
Qualitative PCR of transgenic components in maize
Detection method
1 range
This standard specifies the sampling, sample preparation and PCR detection methods for genetically modified components in corn.
The screening test of this standard is applicable to the qualitative detection of genetically modified components in corn.
The identification test of this standard applies to maize MON810, Bt11, Event176, T14/T 25, CBH351, GA21 strain transgenic
Qualitative testing of points.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by
Some amendments (not including errata content) or revisions do not apply to this standard. However, parties to agreements based on this standard are encouraged to
Whether the latest version of these files can be used. For undated references, the latest edition applies to this standard.
GB/T 6682 Analytical laboratory water specifications and experimental methods
SN/T 1193 Genetic Testing Laboratory Technical Requirements
SN/T 1194 Detection and sampling method for genetically modified components of plants and their products
Qualitative test method for real-time fluorescent PCR of genetically modified components in SN/T 1204 plants and their processed products
3 Terms, definitions and abbreviations
The following terms, definitions and abbreviations apply to this standard.
3.1
A functional DNA sequence derived from other species that is not native to the species, and which is introduced into the species through various means of introduction.
Line expression so that the species acquires new cultivar characteristics.
3.2
The template gene sequence is first denatured to a single strand by high temperature, and is set according to the template sequence under the action of DNA polymerase and suitable reaction conditions.
The two primers are respectively annealed to the corresponding complementary sequence on the two strands of the template DNA, and then combined with each other, followed by DNA polymerization.
Under the action of the enzyme, four deoxyribonucleic acids (dNTPs) are used as substrates to allow the primers to be extended, and then the denaturation, annealing and extension are repeated.
In one cycle, the gene fragment to be amplified is amplified in geometric multiples.
3.3 Abbreviations
3.3.1 PCR. polymerasechainreaction, referred to as PCR.
3.3.2 DNA. deoxyribonucleic acid, deoxyribonucleic acid.
3.3.3 dNTP. deoxyribonucleosidetriphosphate, deoxynucleoside triphosphate.
3.3.4 dATP. deoxyadeNOSinetriphosphate, deoxyadenosine triphosphate.
3.3.5 dCTP. deoxycytidinetriphosphate, deoxycytidine triphosphate.
3.3.6 dGTP. deoxyguaNOSinetriphosphate, deoxyguanosine triphosphate.
3.3.7 dTTP. deoxythymidinetriphosphate, deoxythymidine triphosphate.
3.3.8 dUTP. deoxyuridinetriphosphate, deoxyuridine triphosphate.