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 SN/T 1178-2003: Plant quarantine. Methods for inspection and identification on colorado potato beetle
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 Basic data             | Standard ID | SN/T 1178-2003 (SN/T1178-2003) |           | Description (Translated English) | Plant quarantine. Methods for inspection and identification on colorado potato beetle |           | Sector / Industry | Commodity Inspection Standard (Recommended) |           | Classification of Chinese Standard | B16 |           | Word Count Estimation | 8,830 |           | Date of Issue | 2003-03-17 |           | Date of Implementation | 2003-09-01 |           | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China |           | Summary | This standard specifies the potato beetle [Leptinotarsa ??decemlineata (Say)] of the quarantine and identification methods. This standard applies to potato beetle quarantine and identification. | SN/T 1178-2003: Plant quarantine. Methods for inspection and identification on colorado potato beetle---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
 Plant quarantine.Methods for inspection and identification on colorado potato beetle
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Phytosanitary potato beetle quarantine
Identification method
Released on.2003-03-17
2003-09-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
 ForewordAppendix A and Appendix B of this standard are normative appendices.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Xinjiang Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Zhang Wei, Ke Yimu, Lu Ping, Zhang Xianglin, Han Dongyan.
This standard is the industry standard for inspection and quarantine issued for the first time.
Phytosanitary potato beetle quarantine
Identification method1 ScopeThis standard applies to the quarantine and identification of potato beetles.2 Principle2.1 Potato beetle Coleoptera, Chrysomelidae, Chrysomelinae, Leek
2.2 Potato beetle distribution, host, morphological characteristics, internal anatomical features, transmission routes and biological learning are the quarantine and identification methods
The law provides the basis.3 solution preparation3.1 10% potassium hydroxide solution. 10 g of potassium hydroxide plus 90 mL of distilled water and shaken. Used for specimen anatomy.
3.2 Formalin solution. 1 part of formalin (containing 40% of formaldehyde) plus 17 parts to 19 parts of distilled water and shaken. Used for the preservation of eggs.
3.3 Ethanol solution. Add 0.5% to 1% glycerol to 75% ethanol. Used for the preservation of adult and larvae.
3.4 Holstein sealing liquid. Put 30g gum arabic into 50mL distilled water and put it in a water bath of 50°C~80°C until the gum is dissolved.
Then,.200 g of hydrated trichloroacetaldehyde and 20 mL of glycerin were added, and the mixture was mixed with a glass rod. For limb anatomy and genital anatomy.4 instruments and appliances4.1 Microscope, dissecting mirror, magnifying mirror.
4.2 Petri dishes, beakers, alcohol lamps, micro scalpels, dissection needles, insect needles, slides, concave slides, coverslips, tweezers, labels, various types of holes
Diameter sieve.
4.3 Insect tanks, insects boxes, insects boxes.
4.4 Specimen box. Adult needle inserts.
5 on-site quarantine
5.1 Spot check
5.1.1 Randomly check the sample.
5.1.2 Number of random inspections. Check according to 0.5% to 5% of the total number of goods. All of the following 10 (including 10) inspections; less than 500
13 to 15 pieces of random inspection; 16 to 20 pieces of 501 to 1000 pieces; 21 to 30 pieces of 1001 to 3000 pieces; 3001 pieces
Above, each additional 500 pieces of inspections is increased by one item (the bulk goods are calculated by one piece of 100kg).
5.1.3 Seeds, seedlings and their products from the infected areas of the Solanaceae plant, and discover suspicious epidemics, increase the number of random inspections, and each batch should be more than 50
Parts (all in less than 50 pieces are inspected).
5.1.4 Suspicious insects were found in the spot check and taken back indoors for inspection and identification.
5.2 On-site quarantine of transport vehicles
5.2.1 Before the car, aircraft or ship arrives at the port of entry, the owner and his agent go to the port inspection and quarantine office for inspection and submit it as required.
 
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