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SN/T 1177-2003 English PDF

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SN/T 1177-2003: Protocol of test for B virus related antibodies in monkey
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PDF similar to SN/T 1177-2003


Standard similar to SN/T 1177-2003

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Basic data

Standard ID SN/T 1177-2003 (SN/T1177-2003)
Description (Translated English) Protocol of test for B virus related antibodies in monkey
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B41
Word Count Estimation 6,652
Date of Issue 2003-03-17
Date of Implementation 2003-09-01
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary This standard specifies the monkey B virus-related antibody detection methods. This standard applies to the monkey B virus-related antibodies.

SN/T 1177-2003: Protocol of test for B virus related antibodies in monkey

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of test for B virus related antibodies in monkey Book of the People's Republic of China Entry and Exit Inspection and Quarantine Monkey B virus related antibody detection method Released on.2003-03-17 2003-09-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A of this standard is a normative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted by the Yunnan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Xu Weijia, Dong Jun, Zhou Xiaoli, Xu Zizhong. This standard is the industry standard for inspection and quarantine issued for the first time. Monkey B virus related antibody detection method

1 Scope

This standard specifies the detection method for monkey B virus-related antibodies. This standard applies to the detection of monkey B virus-related antibodies.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 6682-1992 Analytical laboratory water specifications and test methods (eqv ISO 3696.1987)

3 Abbreviations

The following abbreviations apply to this standard. 3.1 CPE Caused by cytopathic effects. 3.2 TCID50 Half of the tissue culture infects. 3.3 Slide EIA Slides enzyme-linked immunosorbent assay. 3.4 SPA Staphylococcal protein A. 3.5 HRP Horseradish peroxidase.

4 Principle

The basic principle of the slide EIA method is that the enzyme molecule is covalently bound to the antibody or antibody molecule, and this binding does not change the immunology of the antibody. It does not affect the biological activity of the enzyme. This enzyme specifically binds to an antigen or antibody adsorbed on a solid support. Drop bottom After the solution, the substrate can be converted into a colored oxidized form by a colorless reduced form of the hydrogen donor contained by the enzyme, and a color reaction occurs. because Therefore, the color reaction of the substrate can be used to determine whether there is a corresponding immune reaction, the depth of the color reaction and the amount of the corresponding antibody or antigen in the sample. It is proportional.

5 reagents and materials

The following reagents refer to analytically pure reagents unless otherwise specified.

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