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SN/T 1173-2015 English PDF

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SN/T 1173-2015: Quarantine protocol for avian viral arthritis
Status: Valid

SN/T 1173: Evolution and historical versions

Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
SN/T 1173-2015English329 Add to Cart 3 days [Need to translate] Quarantine protocol for avian viral arthritis Valid SN/T 1173-2015
SN/T 1173-2003English239 Add to Cart 3 days [Need to translate] Protocol of detection antibodies against avian viral arthritis virus. Enzyme-linked immunosobert assay (ELISA) Obsolete SN/T 1173-2003

PDF similar to SN/T 1173-2015


Standard similar to SN/T 1173-2015

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Basic data

Standard ID SN/T 1173-2015 (SN/T1173-2015)
Description (Translated English) Quarantine protocol for avian viral arthritis
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B41
Word Count Estimation 14,189
Date of Issue 2015-12-04
Date of Implementation 2016-07-01
Older Standard (superseded by this standard) SN/T 1173-2003
Regulation (derived from) AQSIQ Announcement 2015 No.4th batch 120 items of Industry Standards
Issuing agency(ies) General Administration of Customs

SN/T 1173-2003: Protocol of detection antibodies against avian viral arthritis virus. Enzyme-linked immunosobert assay (ELISA)


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of detection antibodies against avian viral arthritis virus. Enzyme-linked immunosobert assay (ELISA) Book of the People's Republic of China Entry and Exit Inspection and Quarantine Chicken viral arthritis antibody detection method Enzyme-linked immunosorbent assay Released on.2003-03-17 2003-09-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A of this standard is a normative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Zhong Anqing, Yang Jianzhong, Lu Tiekang. This standard is the industry standard for inspection and quarantine issued for the first time. Chicken viral arthritis antibody detection method Enzyme-linked immunosorbent assay

1 Scope

This standard specifies a method for the detection of anti-chicken viral arthritis antibodies in chicken serum by enzyme-linked immunosorbent assay (ELISA). This standard applies to epidemiological investigation, diagnosis, quarantine and epidemic monitoring of anti-chicken viral arthritis.

2 Method of operation

2.1 Principle This standard uses the ELISA indirect method. The method utilizes antigen-antibody reaction with specificity, specific chicken viral arthritis After the ELISA antigen is adsorbed onto the surface of the solid phase carrier, if the test substance contains the corresponding anti-chicken viral arthritis virus antibody, The coated antigen binds and continues to form a complex with the corresponding goat anti-chicken enzyme-labeled antibody. When encountering the corresponding substrate, The enzyme in the complex catalyzes the substrate reaction. The color depth of the color is proportional to the amount of the corresponding antibody. It can be characterized by the depth of the color reaction. It can also be quantified in the case of establishing a standard curve (this standard is only for qualitative use). 2.2 Reagents and materials 2.2.1 Reagents See Appendix A for recipes. a) Coating solution. 0.1 mol/L carbonate buffer (pH 9.6). b) Washing solution. 0.01 mol/L phosphate buffer (PBS, pH 7.2~) containing 0.5 mol/L sodium chloride and 0.1% Tween-20 7.4). c) Blocking solution. TNE solution containing 5% yak serum and 0.1% Tween-20. d) Substrate solution. citrate-phosphate buffer (pH 5.0) containing 0.04% o-phenylenediamine (OPD), 0.05% hydrogen peroxide. e) Stop solution. 2 mol/L sulfuric acid solution. f) chicken viral arthritis ELISA antigen, goat anti-chicken IgG enzyme-labeled antibody, positive reference serum and negative reference serum. both by authority Provided by an institutional or nationally recognized manufacturer. 2.2.2 Equipment Polystyrene micro-reaction plate (40-well or 96-well), enzyme-labeled detector, micropipette, plastic dripper, 50μL or 100μL micro-note Projector. 2.3 Method of operation 2.3.1 Coating The chicken viral arthritis ELISA antigen was diluted to the working concentration with a coating solution, 100 μL per well, and placed in a 37 ° C water bath for 1 h before repositioning Leave at 4 ° C overnight. 2.3.2 Washing Dry the coating solution, add.200 μL of blocking solution to each well, place it in a 37 ° C water bath for 90 min, dry it, wash it three times with the washing solution, and dry it. 2.3.3 Adsorption The serum to be tested was diluted 100-fold with blocking solution, 100 μL per well was added, and each serum was made into two wells, placed in a 37 ° C water bath for 1 h, dried and washed. Wash the polyester solution five times and dry it. Positive reference serum and negative reference serum were added to the last row of each plate.

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