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SN/T 1171.2-2003: Detection of antibodies against caprine arthritis-encephalitis virus (CAEV). Agar gel immunodiffusion (AGID)
Status: Obsolete
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Detection of antibodies against caprine arthritis-encephalitis virus (CAEV). Agar gel immunodiffusion (AGID)
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SN/T 1171.2-2003
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Basic data | Standard ID | SN/T 1171.2-2003 (SN/T1171.2-2003) | | Description (Translated English) | Detection of antibodies against caprine arthritis-encephalitis virus (CAEV). Agar gel immunodiffusion (AGID) | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Word Count Estimation | 5,568 | | Date of Issue | 2003-03-17 | | Date of Implementation | 2003-09-01 | | Adopted Standard | OIE/chapter2.4.4; NEQ | | Regulation (derived from) | State inspection recognized [2011] No. 90 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China | SN/T 1171.2-2003: Detection of antibodies against caprine arthritis-encephalitis virus (CAEV). Agar gel immunodiffusion (AGID)
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Detection of antibodies against caprine arthritis-encephalitis virus (CAEV).Agar gel immunodiffusion (AGID)
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Goat arthritis-encephalitis antibody detection method
Agar immunodiffusion test
Released on.2003-03-17
2003-09-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with OIE/chapter 2.4.4 Diagnostic Test and Vaccine Standards Manual.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Yang Baohua, Cui Lu, Yan Jie, Zeng Xiaoping.
This standard is the first industry standard for entry-exit inspection and quarantine.
Goat arthritis-encephalitis antibody detection method
Agar immunodiffusion test
1 Scope
This standard specifies a method for detecting anti-goat arthritis-encephalitis virus antibodies in goat serum using agar immunodiffusion assay.
This standard applies to epidemiological investigation, diagnosis, quarantine and epidemic monitoring of goat arthritis-encephalitis.
2 Test principle
Agar is a polysaccharide containing a sulfate group. It dissolves in water at high temperature, solidifies after cooling, forms a gel, and the agar gel has a porous structure.
The pore size depends on the concentration of the agar. The 1% agar gel has a pore size of about 85 nm, and this pore size range allows for soluble antigen-antibody fractions.
The child diffuses freely in the agar gel and forms a concentration gradient from near to far. When the two meet at a proper ratio, a precipitation reaction occurs and forms a shape.
Form a precipitation zone. Determine whether there is a corresponding antigen-antibody immune response based on the presence or absence of a precipitation zone to determine whether a sample is present in the sample.
Corresponding antibodies or antigens.
3 Instrument and test preparation
3.1 CAE agar diffusion antigen and positive control serum
Provided by the designated unit.
3.2 Preparation of agarose gel plate
Dissolve 0.9% to 1% agarose in Tris-HCl buffer and prepare the plate. Tris 0.605g and sodium chloride (NaCl) 8.0g dissolved
100mL distilled water, followed by 1mol/L hydrochloric acid (HCl) solution to adjust the pH to 7.2, then add 0.9g ~ 1g agarose, boiled by water
Dissolve or dissolve under high pressure, cool to about 55 °C, take about 15mL and pour into a 9cm diameter plate to make agarose gel plate. After cooling, keep it at 4°C.
Save for backup.
3.3 Sampling and sample processing
Under the sterile conditions, 5 mL of blood was collected from the vein, and the blood samples were separated by serum, and then inactivated after being inactivated in a water bath at 56 ° C for 30 minutes.
3.4 Instruments
Balance (0.001g), water bath, agar gel puncher.
4 steps
4.1 Punch
The cooled agarose gel plate was punched with a punching die according to the following illustration, with a hole diameter of 5 mm and a hole pitch of 3 mm. After punching
The bottom of the plate passes over the flame of the alcohol lamp and is back and forth 5 to 7 times (for glass plates). The holes to be punched are numbered as shown in Figure 1.
(1~6).
Figure 1
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