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SN/T 1167-2002 English PDF

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SN/T 1167-2002: Protocol of agar gel immunodiffusion test for epizootic haemorrhagic disease of deer
Status: Obsolete

Standard ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status	PDF
SN/T 1167-2002	English	199	Add to Cart	2 days [Need to translate]	Protocol of agar gel immunodiffusion test for epizootic haemorrhagic disease of deer	Obsolete	SN/T 1167-2002

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Basic data

Standard ID	SN/T 1167-2002 (SN/T1167-2002)
Description (Translated English)	Protocol of agar gel immunodiffusion test for epizootic haemorrhagic disease of deer
Sector / Industry	Commodity Inspection Standard (Recommended)
Classification of Chinese Standard	B41
Word Count Estimation	5,564
Date of Issue	2002-11-25
Date of Implementation	2003-05-01
Issuing agency(ies)	General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary	This standard specifies the deer epizootic haemorrhagic disease agar immunodiffusion test procedures. This standard applies to animal deer epizootic haemorrhagic disease AGP antibodies.

SN/T 1167-2002: Protocol of agar gel immunodiffusion test for epizootic haemorrhagic disease of deer

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of agar gel immunodiffusion test for epizootic haemorrhagic disease of deer People's Republic of China entry and exit inspection and quarantine industry standards SN/T 1167-2002 Deer epidemic hemorrhagic disease agar immunodiffusion test Operating procedure Released on November 11,.2002 2003-05-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard is based on the operation rules of the International Organization for Animal Diseases (OIE) and the National Veterinary Laboratory of the United States and summarizes our country in this field for many years. Based on practical experience. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Yunnan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Yang Jingyan, Ying Xuesong, Yang Jianming, Xu Zizhong. This standard is the industry standard for inspection and quarantine issued for the first time. SN/T 1167-2002 Deer epidemic hemorrhagic disease agar immunodiffusion test Operating procedure

1 Scope

This standard specifies the operating procedures for the agar immunodiffusion test for deer epidemic hemorrhagic disease. This standard is applicable to the detection of agar expansion antibodies in animal deer epidemic hemorrhagic disease.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 6682-1992 Analytical laboratory water specifications and test methods (eqv ISO 3696.1987)

3 Abbreviations

The following abbreviations apply to this standard. 3.1 Agar immunodiffusion test. 3.2 EHD Deer epidemic hemorrhagic disease.

4 Principle

The antigen and its specific antibody have little resistance when diffused from the high concentration region to the low concentration region in the gel, and are basically freely expanded. Form of dispersion. Due to the different molecular weight, structure, shape and charge of different antigen molecules, their diffusion coefficients are different and their diffusion in the gel The speed will be different. When the antigen and the corresponding antibody meet in the gel after diffusion, form an antigen-antibody complex, if the ratio of the two at the meeting When appropriate, the largest composite is formed. As the molecular weight of the composite increases, the particles increase, so that no further diffusion occurs and precipitation occurs. A linear or band-shaped "specific barrier" formed by a line or band is called an immunoprecipitation line or an immunoprecipitation zone.

5 reagents and materials

5.1 Antigen. EHDV-2 international standard strain introduced from the National Veterinary Laboratory of the United States, refer to the antigen preparation method of the laboratory Preparation, for group-specific antigens, for quarantine of each type of EHD, provided by the designated unit. 5.2 Standard positive serum. The agar diffusion titer is above 1.8 and is provided by the designated unit. 5.3 plates. 5.4 Micropipettes. 5.5 Micro dripper. 5.6 Erlenmeyer flask. SN/T 1167-2002

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