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SN/T 1166.2-2002 English PDF

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SN/T 1166.2-2002: Protocol of micro-serum neutralization test for vesicular stomatitis
Status: Obsolete

Standard ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status	PDF
SN/T 1166.2-2002	English	279	Add to Cart	3 days [Need to translate]	Protocol of micro-serum neutralization test for vesicular stomatitis	Obsolete	SN/T 1166.2-2002

PDF similar to SN/T 1166.2-2002

Standard similar to SN/T 1166.2-2002

GB/T 20365 SN/T 1353 NY/T 909 SN/T 1168 SN/T 1162 SN/T 1166

Basic data

Standard ID	SN/T 1166.2-2002 (SN/T1166.2-2002)
Description (Translated English)	Protocol of micro-serum neutralization test for vesicular stomatitis
Sector / Industry	Commodity Inspection Standard (Recommended)
Classification of Chinese Standard	B41
Word Count Estimation	7,767
Date of Issue	11/25/2002
Date of Implementation	5/1/2003
Regulation (derived from)	State-Quality-Inspection-accreditation (2010) 582
Issuing agency(ies)	General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China

SN/T 1166.2-2002: Protocol of micro-serum neutralization test for vesicular stomatitis

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of micro-serum neutralization test for vesicular stomatitis People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Buccal stomatitis microserum neutralization test procedure Released on November 11,.2002 2003-05-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

VesicuLarStomatitis (VS) serum neutralization test is in reference to the International Organization for Animal Diseases (OIE) "Mammals, Avian and Based on the bee A and B disease diagnostic test and vaccine standard manual and practical experience. Appendix A of this standard is an informative annex. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Yunnan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Hua Qunyi, Zhou Xiaoli, Xu Zizhong, Yang Jingyan. This standard is the industry standard for inspection and quarantine issued for the first time. Buccal stomatitis microserum neutralization test procedure

1 Scope

This standard specifies the neutralization test method for vesicular stomatitis virus. This standard applies to animal vesicular stomatitis virus antibodies and stereotypes detection.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 6682-1992 Analytical laboratory water specifications and test methods (eqv ISO 3696.1987)

3 Abbreviations

The following abbreviations apply to this standard. 3.1 CPE. cytopathic effect. 3.2 TCID50. Half of the tissue culture infection volume. 3.3 VSV. vesicular stomatitis virus. 3.4 VSV-NJ. vesicular stomatitis virus New Jersey type; VSV-IND vesicular stomatitis virus Indiana type.

4 Principle

When a specific serum neutralizing antibody binds to a virus, it can inhibit the adsorption of the virus on sensitive cells, thereby losing the ability to infect, thereby Prevent the replication of the virus in the cell. This reaction is not only manifested as a virus that can only be neutralized by the corresponding immune serum, but also In order to neutralize a certain amount of virus (infectivity), there must be a certain titer of immune serum. The neutralization test is based on the determination of the infectivity of the virus. The virus-susceptible cells should be selected as the material, and the titer of the neutralizing antibody should be compared to compare the residual infectivity of the virus after neutralizing the immune serum. according to. Animals can produce specific antibodies 4 to 5 days after infection with vesicular stomatitis virus. Neutralizing antibodies have a high degree of specificity, Therefore, the type identification can be performed by the neutralization test.

5 reagents and materials

5.1 Virus. VSV-NJ and VSV-IND international standard strain, provided by the designated unit. 5.2 Serum. VSV two types of standard positive serum, negative serum, provided by the designated unit. 5.3 Tested serum. Bovine and pig serum were inactivated at 60 ° C for 30 min. Horse serum was inactivated at 65 ° C for 30 min. 5.4 Cells. BHK21 or Vero cell lines. 5.5 Culture medium. E-MEM cell growth solution, see Appendix A. 5.6 Maintenance and Diluent. E-MEM Cell Maintenance, see Appendix A. 5.7 Cell dispersion. See Appendix A. 5.8 Water. It meets the secondary water specification of the analytical laboratory of GB/T 6682-1992.

6 instruments and equipment

Analytical balance, autoclave, dry oven, ultrasonic pulverizer, inverted microscope, micro-oscillator, carbon dioxide incubator, refrigerator (Preserved serum at -20 ° C, preserved at -70 ° C). 96-well tissue culture plate, single-channel, multi-channel micropipette (20μL ~.200μL).

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