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SN/T 1166-2010: Quarantine protocol for vesicular stomatitis
Status: Valid SN/T 1166: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1166-2010 | English | 469 |
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Quarantine protocol for vesicular stomatitis
| Valid |
SN/T 1166-2010
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| SN/T 1166.1-2002 | English | 319 |
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Protocol of complement fixation test for vesicular stomatitis
| Obsolete |
SN/T 1166.1-2002
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PDF similar to SN/T 1166-2010
Basic data | Standard ID | SN/T 1166-2010 (SN/T1166-2010) | | Description (Translated English) | Quarantine protocol for vesicular stomatitis | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 18,177 | | Date of Issue | 2010-11-01 | | Date of Implementation | 2011-05-01 | | Older Standard (superseded by this standard) | SN/T 1166.1-2002; SN/T 1166.2-2002; SN/T 1166.3-2006 | | Quoted Standard | GB/T 18088 | | Regulation (derived from) | National Quality Inspection (2010) 582 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the animal vesicular stomatitis virus isolation and identification, reverse transcription polymerase chain reaction, fluorescence reverse transcription polymerase chain reaction, neutralization test, competitive enzyme-linked immunosorbent assay Zhu surgery requirements. This standard applies to vesicular stomatitis animal inspection and quarantine. | SN/T 1166.1-2002: Protocol of complement fixation test for vesicular stomatitis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of complement fixation test for vesicular stomatitis
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Blister stomatitis complement binding test procedure
Released on November 11,.2002
2003-05-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
Vesicular stomatitis (VS) complement fixation test in reference to the International Organization for Animal Disease (OIE) "mammals, poultry and honey"
Based on the bee A and B disease diagnostic tests and vaccine standards manual and practical experience.
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Yunnan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Hua Qunyi, Zhou Xiaoli, Xu Zizhong.
This standard is the industry standard for inspection and quarantine issued for the first time.
Blister stomatitis complement binding test procedure
1 Scope
This standard specifies the method for complementation of vesicular stomatitis.
This standard applies to animal vesicular stomatitis virus antigen detection.
2 Normative references
The terms in the following documents become the terms of this standard by reference to the standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to
Whether the latest version of these files can be used. For undated references, the latest edition applies to this standard.
GB/T 6682-1992 Analytical laboratory water specifications and test methods (eqv ISO 3696.1987)
3 Abbreviations
The following abbreviations apply to this standard.
3.1 CF. complement binding assay.
3.2 CH50. 50% complement hemolysis unit.
3.3 VSV. vesicular stomatitis virus.
3.4 VSV-NJ. vesicular stomatitis virus New Jersey type; VSV-IND vesicular stomatitis virus Indiana type.
3.5 VBD. Barbital buffer.
3.6 PBS. phosphate buffer.
4 Principle
The complement fixation assay consists of two systems, the first system being the reaction system, known as the antigen (or antibody), the serum (or antigen) being tested and
complement. The second system is an indicator system (also known as a hemolysis system), namely hemolysin plus sheep red blood cells, which is an anti-sheep red blood cell antibody. Make up
The guinea pig serum is commonly used, which has strong lysis ability to red blood cells. Complement can only bind to antigen-antibody complexes and be activated
Hemolysis. Therefore, if the antigen and antibody in the test system are corresponding, an immune complex is formed and the quantitative complement is bound.
At this time, the indicator system is added, and due to the lack of free complement, no hemolysis occurs, which is a positive reaction. On the contrary, the test system lacks antigen or special
The heterologous antibody, which cannot form the immune complex complement, is freed from the reaction solution, and is indicated by the system, namely hemolysin plus sheep red blood cell immune complex
The substance is activated, and hemolysis occurs, that is, a negative reaction.
5 reagents and materials
5.1 Hemolysin. provided by the designated unit. Rabbit anti-sheep erythrocyte serum can also be obtained by continuously immunizing rabbits with 50% sheep red blood cells.
Hemolysin.
5.2 Complement. Provided by the designated unit. Healthy guinea pig serum can also be collected, and 2 to 3 guinea pig serums are mixed and divided into small tubes.
1mL, placed at -25 ° C below the low temperature freeze preservation use.
5.3 Preparation of 2.8% sheep red blood cell suspension. Sheep red blood cells stored in albino solution can be purchased directly. You can also collect blood by yourself and save it in
In the solution of A. Wash three times with cold VBD before use until the supernatant is clear. If the supernatant is not cleared by three centrifugations, it should be replaced.
New batch of red blood cells. Take the pelleted sheep red blood cells (according to the scale on the centrifuge tube) 2.8mL, add 97.2mL VBD,
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