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Protocol of ELISA for infectious bovine rhinotracheitis
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SN/T 1164.3-2006
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Basic data | Standard ID | SN/T 1164.3-2006 (SN/T1164.3-2006) | | Description (Translated English) | Protocol of ELISA for infectious bovine rhinotracheitis | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 7,714 | | Date of Issue | 2006-01-26 | | Date of Implementation | 2006-08-16 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Industry Standard Notice 2006 No. 4 (total of 76) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the infectious bovine rhinotracheitis ELISA method. This standard applies to infectious bovine rhinotracheitis antibodies. | SN/T 1164.3-2006: Protocol of ELISA for infectious bovine rhinotracheitis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of ELISA for infectious bovine rhinotracheitis
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Bovine infectious rhinotracheitis enzyme-linked immunosorbent assay
Operating procedure
Released on.2006-01-26
Implementation of.2006-08-16
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
SN/T 1164 is divided into four parts.
--- Operational procedures for the isolation of bovine infectious rhinotracheitis virus;
--- Bovine infectious rhinotracheitis trace serum neutralization test procedures;
--- Bovine infectious rhinotracheitis enzyme-linked immunosorbent assay operating procedures;
--- Bovine infectious rhinotracheitis virus detection and identification method PCR method.
This part is the third part of SN/T 1164.
Appendix A of this section is a normative appendix.
This part is proposed and managed by the National Certification and Accreditation Administration.
This section drafted by. Yunnan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this section. Xiao Ronghai, Zhou Xiaoli, Xu Weijia, Yang Yanxiong, Ying Xuesong.
This section is the first industry standard for entry-exit inspection and quarantine.
Bovine infectious rhinotracheitis enzyme-linked immunosorbent assay
Operating procedure
1 Scope
This part of SN/T 1164 specifies an enzyme-linked immunosorbent assay for bovine infectious rhinotracheitis.
This section applies to the detection of bovine infectious rhinotracheitis antibodies.
2 Normative references
The provisions in the following documents become the provisions of this part by reference to this part of SN/T 1164. Quotations with dated
, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouragement is achieved under this section
The parties to the agreement study whether the latest versions of these documents can be used. For undated references, the latest edition applies to this
section.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Abbreviations
The following abbreviations apply to this part of SN/T 1164.
3.1
IBR
3.2
ELISA
4 reagents and materials
4.1 Coating antigen. provided by the designated unit.
4.2 Serum. Standard strong positive, negative serum, provided by the designated unit.
4.3 Serum to be tested. bovine serum was inactivated at 56 ° C ~ 57 ° C for 30 min, and goat serum was inactivated at 58 ° C ~ 59 ° C for 30 min.
4.4 Enzyme-labeled anti-antibody. supplied by the designated unit.
4.5 Solution. antigen coating buffer, blocking solution, washing solution, substrate solution and stop solution, see Appendix A.
4.6 Water. Meet the secondary water specifications of the GB/T 6682 analytical laboratory.
5 instruments and equipment
5.1 Microplate reader.
5.2 polystyrene microplate (96-well microtiter plate).
5.3 Single-channel, multi-channel adjustable micropipettes (1μL, 50μL, 100μL, 1000μL), micro dripper.
5.4 Pipette (5mL, 10mL).
5.5 Graduated cylinder (100mL, 1000mL).
5.6 Reagent bottles (50mL, 100mL, 500mL).
5.7 Wash bottle (500 μL).
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