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SN/T 1151.6-2004 English PDF

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SN/T 1151.6-2004: Shrimp white spot virus dot blot hybridization and in situ hybridization operating procedures
Status: Obsolete
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SN/T 1151.6-2004English519 Add to Cart 4 days [Need to translate] Shrimp white spot virus dot blot hybridization and in situ hybridization operating procedures Obsolete SN/T 1151.6-2004

PDF similar to SN/T 1151.6-2004


Standard similar to SN/T 1151.6-2004

GB 5009.261   SN/T 1151.5   SN/T 1151.3   SN/T 1151.1   

Basic data

Standard ID SN/T 1151.6-2004 (SN/T1151.6-2004)
Description (Translated English) Shrimp white spot virus dot blot hybridization and in situ hybridization operating procedures
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B50
Classification of International Standard 67.120.30
Word Count Estimation 13,156
Date of Issue 11/17/2004
Date of Implementation 2005-04-01
Regulation (derived from) 2005 Bulletin No. 3 industry standard
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary This standard specifies the white spot virus nucleic acid probe techniques dot blot and in situ hybridization. This standard applies to white spot virus detection and identification.

SN/T 1151.6-2004: Shrimp white spot virus dot blot hybridization and in situ hybridization operating procedures


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Shrimp white spot virus dot blot hybridization and in situ hybridization operating procedures Book of the People's Republic of China Entry and Exit Inspection and Quarantine Spotted hybridization and in situ hybridization of shrimp white spot virus Test procedure Released on November 11,.2004 2005-04-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A of this standard is a normative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Qingdao Entry-Exit Inspection and Quarantine Bureau, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences. The main drafters of this standard. Liu Wei, Lei Wenwen, Jiang Yulin, Yang Bing, Gao Longying, Shi Xiujie, Huang Wei. This standard is the first industry standard for entry-exit inspection and quarantine. Practice on dot blot hybridization and in situ hybridization detection of shrimp white spot virus

1 Scope

This standard specifies the nucleic acid probe detection technology of shrimp white spot virus---hpot hybridization and in situ hybridization. This standard applies to the detection and identification of white spot virus.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 6682-1992 Analytical laboratory water specifications and test methods

3 reagents and materials

3.1 If the water used is not specified, it should meet the specifications of the first grade water in GB/T 6682-1992. 3.2 If not stated, this standard uses reagents identified as analytically pure. 3.3 DIG-labeled nucleic acid probe. according to the national invention patent ZL111336.4 (the patentee is the Yellow Sea Water of China Academy of Fishery Sciences) Technology Institute)). 3.4 SEMP-tris slurry. According to the national invention patent ZL111336.4 (the patentee is the Yellow Sea Fisheries of China Academy of Fishery Sciences) Institute of Technology). 3.5 20×SSC. See section A. Chapter 1. 3.6 5×SSC. See section A. Chapter 2. 3.7 2×SSC. See section A. Chapter 3. 3.8 1×SSC. See section A. Chapter 4. 3.9 0.5×SSC. See section A. Chapter 5. 3.10 10×BufferI. See section A. Chapter 6. 3.11 1×BufferI. See section A. Chapter 7. 3.12 BufferII (BlockingBuffer). See section A. Chapter 8. 3.13 10% sodium dodecyl sulfate (SDS). see section A. Chapter 9. 3.14 2×SSC/0.1% SDS. See section A. Chapter 10. 3.15 0.1 × SSC/0.1% SDS. see section A. Chapter 11. 3.16 Pre-hybrid solution. see section A. Chapter 12. 3.17 10×BufferIII. See section A. Chapter 13. 3.18 1×BufferIII. See section A. Chapter 14. 3.19 Alkaline phosphatase-labeled DIG antibody. 150 U (200 μL), product of Boehringer Mannheim, Germany or equivalent product. 3.20 75mg/mL nitroblue tetrazolium solution. see section A. Chapter 15. 3.21 50mg/mL 5-bromo-4-chloro-3-indolyl phosphate-toluidine salt solution. see section A. Chapter 16. 3.22 Coloring solution I. See section A. Chapter 17. 3.23 Davidson's AFA Fixative. See Section A. Chapter 18.

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