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SN/T 1129.2-2002: Protocol of virus isolation for bovine viral diarrhea/mucosal disease
Status: Obsolete
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1129.2-2002 | English | 319 |
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Protocol of virus isolation for bovine viral diarrhea/mucosal disease
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SN/T 1129.2-2002
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Basic data | Standard ID | SN/T 1129.2-2002 (SN/T1129.2-2002) | | Description (Translated English) | Protocol of virus isolation for bovine viral diarrhea/mucosal disease | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Word Count Estimation | 8,855 | | Date of Issue | 8/2/2002 | | Date of Implementation | 1/1/2003 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China | SN/T 1129.2-2002: Protocol of virus isolation for bovine viral diarrhea/mucosal disease---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of virus isolation for bovine viral diarrhea/mucosal disease
People's Republic of China entry and exit inspection and quarantine industry standards
Operating procedure
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
The method for virus isolation and identification in this standard refers to the testing procedures of the US and Australian laboratories and summarizes the country in this
Developed on the basis of years of research and practical experience in the field.
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard is drafted by. Yunnan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Zhou Xiaoli, Hua Qunyi, Xu Zizhong, Xu Weijia.
This standard is the first industry standard for entry-exit inspection and quarantine.
range
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, all subsequent
Modifications (not including errata content) or revisions do not apply to this standard, however, research that encourages agreements based on this standard is
No, the latest version of these files can be used. For undated references, the latest edition applies to this standard.
Principle
After the animal is infected with the BVD virus and produces antibodies, there are viruses in many tissues of the diseased cow for a long period of time, acute cases.
It has sustained viremia, so blood is the best virus separation material, taking a coagulated blood clot and using it as an inoculum after several freeze-thaw cycles. corpse
Mesenteric lymph nodes or spleen are taken during body necropsy, and bone marrow can be used as an inoculum after routine treatment. Long-term or suspected antibodies
When present, the virus may be isolated by using the above-mentioned disease materials, and may be interfered with. At this time, anticoagulation may be taken, and it is more desirable to isolate white blood cells as an inoculum.
When examining local infections, a cotton swab can be used to take local secretions or excretions.
Various bovine source cells can be used for virus isolation, including bovine kidney and fetal bovine primary or secondary cells and yak testicular cells. Yak
Primary testicular cells often show better cytopathic effects.
If the isolate causes cytopathic effects, it should be further identified by neutralization test with anti-BVDV immune serum. If there is no cytopathic effect, then
Identification was carried out by immunofluorescence antibody test (FA), immunoperoxidase test, neutralization test and the like.
The disease material should be passed on sensitive cells for at least two generations before it can be identified.
. Virus. BVD virus OregonC24V.
. Serum. BVD standard positive serum, negative serum.
. Samples to be tested. coagulated blood clots, mesenteric lymph nodes or spleen, bone marrow, white blood cells, secretions or excretions, after several freezes and freezes
As an inoculum. If long-distance transportation is required, the material should be placed in a transport solution containing high concentrations of antibiotics (5000 IU of penicillin per ml,
5000 μg streptomycin and 10 μg amphotericin B).
. Cell. The cells used in the experiment are primary or secondary yak kidney cells or testicular cells, generally no more than four generations, according to conventional trypsin digestion
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