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SN/T 1110-2002: Procedure of virus isolation and identification for Newcastle disease
Status: Obsolete
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1110-2002 | English | 319 |
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Procedure of virus isolation and identification for Newcastle disease
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SN/T 1110-2002
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PDF similar to SN/T 1110-2002
Basic data | Standard ID | SN/T 1110-2002 (SN/T1110-2002) | | Description (Translated English) | Procedure of virus isolation and identification for Newcastle disease | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Word Count Estimation | 8,834 | | Date of Issue | 2002-05-20 | | Date of Implementation | 2002-11-01 | | Regulation (derived from) | State inspection recognized [2011] 291 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China | SN/T 1110-2002: Procedure of virus isolation and identification for Newcastle disease---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Procedure of virus isolation and identification for Newcastle disease
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Newcastle disease virus isolation and identification method
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Implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
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Foreword
This standard is equivalent to the International Veterinary Bureau's standard "Diagnostic Methods and Vaccine Standards Manual" (2000, fourth edition), and reference to the Ministry of Agriculture
The Newcastle Disease Quarantine Technical Specification is compiled and formulated in conjunction with our operational experience.
Appendix A and Appendix B of this standard are appendices to the standard.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
Drafters of this standard. Shan Songhua, Hu Yongqiang.
This standard is the first industry standard for entry-exit inspection and quarantine.
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Newcastle disease virus isolation and identification method
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The State Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
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This standard specifies the isolation and identification methods for avian Newcastle disease virus (NDV).
This standard applies to the isolation and identification of avian Newcastle disease virus.
The provisions contained in the following standards constitute provisions of this standard by reference in this standard. At the time of publication of this standard, the versions shown are
To be effective. All standards will be modified and parties using this standard should explore the possibility of using the latest version of the following standards.
OIE "Handbook of Diagnostic Methods and Vaccine Standards" (2000, fourth edition)
Material preparation
The reagents used were of analytical grade.
. Ordinary balance, analytical balance, ordinary centrifuge, V-shaped blood coagulation plate, micro-oscillator, 50μL adjustable pipette, incubator, ultra-clean work
Taiwan, plastic blood collection tubes, etc.
. Antibiotics. penicillin, streptomycin, gentamicin and bacteriocin.
. No specific pathogen (SPF) chicken embryo, 1 day old SPF chicken and 6 week old SPF chicken at 9 to 11 days old.
. pH 7.0 or to pH 7.4 0.01 mol of stewed L-phosphate buffer (PBS) (see Appendix A of the Appendix).
. Preparation of A's solution (red blood cell preservation solution) (see Appendix B of the Appendix B standard).
. 1% chicken red blood cells. adult chicken blood collected from SPF chicken or no Newcastle disease hemagglutination inhibitory antibody, placed in the same amount of albino solution, 20 times
The volume of PBS was washed 3 to 4 times, and each time was centrifuged at.2000 rpm for 3 to 4 minutes, and the last time was 5 minutes, and 1% suspension was prepared with PBS.
The suspension was stored at 4 ° C and used within 72 h.
. Swab preparation. Wrap the absorbent cotton around the blunt end of the toothpick to form a 0.4cm diameter cotton wool ball, then wrap it in kraft paper, each bundle
10, autoclaved at 121 ° C for 15 min, ready for use. Or buy a swab with the product directly.
. Standard Newcastle disease virus, standard positive serum. supplied by the designated unit.
. Sample collection
.. Live poultry collect tracheal swabs and cloaca swabs (with feces); chicks collect fresh feces.
.. Dead birds collect snouts, lungs, kidneys, intestines (including contents), spleen, brain, liver, heart and other tissues. Samples can be collected or mixed separately, intestines.
The contents often need to be handled separately.
.. Bone marrow can be collected from a long-term ND diseased chicken or atypical ND case.
.. Put the aseptically collected sample into a sterile vial and mark it clearly (number, sampling time), and the sample is immediately sent to the laboratory for treatment or
Store frozen at -30 °C.
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