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SN/T 1022-2010: Detection of Vibrio cholerae in food for import and export
Status: Valid SN/T 1022: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1022-2010 | English | 369 |
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Detection of Vibrio cholerae in food for import and export
| Valid |
SN/T 1022-2010
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| SN/T 1022-2001 | English | 479 |
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Method for the determination of vibrio cholerae in food for export
| Obsolete |
SN/T 1022-2001
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PDF similar to SN/T 1022-2010
Basic data | Standard ID | SN/T 1022-2010 (SN/T1022-2010) | | Description (Translated English) | Detection of Vibrio cholerae in food for import and export | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C53 | | Classification of International Standard | 07.100.30 | | Word Count Estimation | 14,180 | | Date of Issue | 2010-01-10 | | Date of Implementation | 2010-07-16 | | Older Standard (superseded by this standard) | SN/T 1022-2001 | | Quoted Standard | SN 0330; SN/T 1538.1; SN/T 1538.2 | | Adopted Standard | ISO/TS 21872-1-2007, NEQ | | Regulation (derived from) | National Quality Inspection (2010) No. 13 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export food inspection method of Vibrio cholerae. This standard applies to the test of Vibrio cholerae in food, animal feed and other food production and processing areas of V. cholerae in environmental samples may refer to the use of the test. | SN/T 1022-2010: Detection of Vibrio cholerae in food for import and export---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of Vibrio cholerae in food for import and export
Exit inspection and quarantine industry standard book People's Republic of China
Instead of the SN/T 1022-2001
Vibrio cholerae in food import and export inspection methods
Issued on. 2010-01-10
2010-07-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Instead of the standard SN/T 1022-2001 "Vibrio cholerae in food for export testing methods."
The standard reference ISO /T S21872-1.2007 "Microbiology of food and feed testing methods Vibrio spp level test
Methods Part I. Vibrio parahaemolyticus and Vibrio cholerae test (Microbiologyoffoodandanimalfeedingstuffs-
Food detection and counting (PathogenicVibriospecies.Detectionandenumerationinfoods) ", the original standard text
Format, wording and text revision.
This standard compared with SN/T 1022-2001, the main changes are as follows.
--- The original standard name revised to "import and export food in V. cholerae test methods";
--- The original scope of the standard has been revised;
--- The revised sample preservation and sample preparation. Increase the preparation of different types of samples in the sample preparation 3.2. 3.3
Save the sample increases the storage method of the sample;
--- A method to increase the feed;
--- Media, reagent formulations were part of the revision of reference ISO /T S21872-1 method;
--- Original standard detection steps were revised by the same method twice enrichment broth for the enrichment of the revised twice selective enrichment party
law. And for processed products, such as heating, freezing, drying or saline samples, select 37 ℃ enrichment culture for the first time, fresh
Samples 41.5 ℃ enrichment culture for the first time;
--- Increased chromogenic medium screening test.
Appendix A of this standard is a normative appendix, Appendix B is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Liaoning Entry Inspection and Quarantine Bureau, People's Republic of China Shandong Entry-Exit Inspection and Quarantine
Bureau, People's Republic of China Jiangxi Exit Inspection and Quarantine.
The main drafters of this standard. Xu Junyi, Juan Cao occasion, Wang Gang, Ray quality Wen Cheng Chiu, Yang Chunhua, Yang Xu, Zhao Xin, Qi Zhenyu, Liushu Yan.
This standard replaces the standards previously issued as follows.
--- SN/T 1022-2001.
Vibrio cholerae in food import and export inspection methods
1 Scope
This standard specifies the test methods in food import and export of Vibrio cholerae.
This standard applies to V. cholera Vibrio cholerae in food inspection, animal feed and other food production and processing area of environmental samples
Bacteria can refer to the use of test.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
SN 0330 export food microbiological examination General
SN/T 1538.1 media preparation Guide - Part 1. General laboratory quality assurance prepared medium
SN/T 1538.2 media preparation Guide Part 2. A Practical Guide to medium performance test
3 Sample preparation and preservation
3.1 Sampling
The number of samples and sampling methods carried out by the SN 0330.
3.2 Sample Preparation
3.2.1 a common sample
Representative samples taken from the composite sample, the edible parts (shelled shellfish shells removal method according to 3.2.2) and mix well. With four
Reduced separation method is not less than 500g as samples loaded into the sterile container, sealed identification.
3.2.2 shelled shellfish
Should be washed in clean running water and housing, with 70% ethanol sterilized then aseptically cut the adductor muscle, open shells. take out
Including all shellfish and shellfish was visceral. Each test sample shall include at least six individual shellfish.
3.3 Sample preservation
Immediately after sample collection at 7 ℃ ~ 10 ℃ preservation, 24h in the test. If the sample to be refrigerated, stored at -18 ℃, 24h within
test. In order to ensure maximum survival of Vibrio should avoid ice in direct contact with the sample.
4 Equipment and Materials
4.1 homogenizer. 8000r/min ~ 10000r/min.
4.2 incubator. 37 ℃ ± 1 ℃.
4.3 incubator or water bath pot. 41.5 ℃ ± 1 ℃.
4.4 temperature water bath. 37 ℃ ± 1 ℃.
4.5 microscope.
5 medium and reagents
5.1 Alkaline peptone water (APW), see Appendix A, Section A. Chapter 1.
5.2 TCBS agar, see Appendix A, Section A. 2.
5.3 CHROMIDVIBRIO vibrio chromogenic medium, see Appendix A, Section A. 3.
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