|
US$289.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
SN/T 0762-2011: Protocol of real-time RT-PCR for detection of classical swine fever virus
Status: Valid SN/T 0762: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 0762-2011 | English | 289 |
Add to Cart
|
3 days [Need to translate]
|
Protocol of real-time RT-PCR for detection of classical swine fever virus
| Valid |
SN/T 0762-2011
|
| SN/T 0762-1999 | English | 479 |
Add to Cart
|
4 days [Need to translate]
|
Rule of inspection for export weaving wool type muffler
| Obsolete |
SN/T 0762-1999
|
PDF similar to SN/T 0762-2011
Basic data | Standard ID | SN/T 0762-2011 (SN/T0762-2011) | | Description (Translated English) | Protocol of real-time RT-PCR for detection of classical swine fever virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Word Count Estimation | 11,138 | | Date of Issue | 2011-05-20 | | Date of Implementation | 2011-12-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | State-Quality-Inspection-Accrediadation (2011) 291; Accredidation-Technology-Letter [2015] No. 247 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the method of operation of classical swine fever virus fluorescent RT-PCR detection. This standard applies to pigs and swine fever virus detection products. | SN/T 0762-2011: Protocol of real-time RT-PCR for detection of classical swine fever virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of real-time RT-PCR for detection of classical swine fever virus
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Classical swine fever virus fluorescent RT-PCR detection method
Issued on. 2011-05-20
2011-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai Fosun Medical Technology Development Co., Ltd.
The main drafters of this standard. Li Chunyang, Liu Junping, Xiong Wei, a single pine Hua, Huang Zhongrong, Xia Yi, Hu Yongqiang, Li Shuqing, Wang Qiao whole.
Classical swine fever virus fluorescent RT-PCR detection method
1 Scope
This standard specifies the method of operation of classical swine fever virus fluorescent RT-PCR detection.
This standard applies to pigs and swine fever virus detection products.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Abbreviations
The following abbreviations apply to this document.
Fluorescent RT-PCR. fluorescent RT - polymerase chain reaction
The number of cycles the amount of fluorescence signal of each reaction tube reaches a set threshold when experienced. Ct values
RNA. ribonucleic acid
DEPC. Coke ethylene carbonate
Taq enzyme. TaqDNA polymerase
Principle 4
Classical swine fever virus is enveloped positive-strand RNA viruses. According to the conserved sequences of different isolates of classical swine fever virus gene, a pair of specific
Primers and a dual-labeled probes specific fluorescence. Probe and conserved sequences of viral genes combine, the binding site is located primers
Area. Probe 5 'end labeled FAM luciferin, it emits can be received detection equipment, the report called fluorophores (with
R represents), 3 'TAMRA labeled fluorescein, which can absorb at close range within the 5' fluorophore reporter fluorescent signal emitted, called quenching
Off fluorophore (denoted by Q). When the PCR reaction into the annealing stage, primers and probes simultaneously with the target gene binding, then probe
R groups on the fluorescence signal emitted is absorbed by the Q group, the instrument can not detect the fluorescence signal; PCR reaction is carried out to the extended stage, Taq
Enzymes play its 5 '→ 3' exonuclease enzyme hydrolysis probe function, at the same time marked on the probe freed R groups, the R
Q fluorescence emitted no longer be absorbed and is received by the detector. With the growth of performing PCR reactions, the PCR product with fluorescence signal
Was correspondence.
5 equipment, materials and reagents
5.1 equipment, materials
5.1.1 PCR fluorescence detector.
5.1.2 high-speed desktop refrigerated centrifuge (centrifugal velocity 12000g above).
5.1.3 ordinary desktop centrifuge (centrifugal speed 3000g).
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 0762-2011_English be delivered?Answer: Upon your order, we will start to translate SN/T 0762-2011_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of SN/T 0762-2011_English with my colleagues?Answer: Yes. The purchased PDF of SN/T 0762-2011_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay. Question 5: Should I purchase the latest version SN/T 0762-2011?Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version SN/T 0762-2011 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically.
|