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SN/T 0330-2012: General rules for microbiological examinations of export food
Status: Valid SN/T 0330: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 0330-2012 | English | 859 |
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6 days [Need to translate]
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General rules for microbiological examinations of export food
| Valid |
SN/T 0330-2012
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| SN 0330-1994 | English | 479 |
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4 days [Need to translate]
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General guidance for microbiologicalexamination of export foods
| Obsolete |
SN 0330-1994
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PDF similar to SN/T 0330-2012
Basic data | Standard ID | SN/T 0330-2012 (SN/T0330-2012) | | Description (Translated English) | General rules for microbiological examinations of export food | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C53 | | Classification of International Standard | 07.100.30 | | Word Count Estimation | 35,352 | | Older Standard (superseded by this standard) | SN 0330-1994 | | Quoted Standard | GB 19489; GB/T 27025; GB/T 27405; SN/T 1538.1; SN/T 1538.2; SN/T 2102.1; ISO/IEC GUIDE 43-1; ISO 6887; ISO 8199; ISO 8261; ISO 14461-2; ISO 14661-2; ISO 16140; ISO/TS 19036 | | Regulation (derived from) | National Quality Inspection (2012) 237 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the particular standard in accordance with the implementation of the general requirements of food microbiological testing. This standard applies to food microbiology laboratory samples of food, food and food ingredients produced en | SN/T 0330-2012: General rules for microbiological examinations of export food---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
General rules for microbiological examinations of export food
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Replacing SN 0330-1994
Export Food Inspection General Microbiology
Issued on. 2012-05-07
2012-11-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Table of Contents
Introduction Ⅲ
1 Scope 1
2 Normative references 1
3 1 Laboratory workplace
3.1 Overview 1
3.2 Safety Requirements 1
3.3 Design Lab 2
3.4 laboratory areas 2
3.5 laboratory workplace layout and facilities 2
3.6 Cleaning and disinfection 3
4 4 operator
4.1 Overview 4
4.2 Ability 4
4.3 verify the ability of the laboratory personnel 4
4.4 Health 4
5 Equipment 4
6 laboratory equipment ready 5
6.1 Preparation 5
6.2 sterilization/decontamination 5
Disposable Supplies and materials 6.3 5
Clean glassware and materials to save 6.4 of 5
6.5 Sterilization of glassware and materials management 5
6.6 Decontamination and disinfection 6
6.7 Waste treatment 6
6.8 Cleaning 6
7 Preparation and sterilization of the medium 6
8 laboratory sample 6
8.1 Sampling 6
8.2 sample transport 7
8.3 sample receiving 7
8.4 Sample Storage 8
8.5 detection section 8
9 9 test
9.1 Inspection health preparedness 9
9.2 Preparation of initial suspension and dilutions 9
10 Count 10
10.1 Overview 10
10.2 10 solid medium count
10.3 solid medium count calculation and expression of results 12
10.4 yeast and mold counts 15
10.5 broth MPN assay 15
Qualitative detection method 19 11
11.1 Overview 19
Principle 19 11.2
11.3 Measurement Uncertainty 20
The method of Claim 12 20
12.1 Overview 20
12.2 Prepare pure cultures of 20
12.3 Gram staining 20
12.4 biochemically confirmed 20
12.5 Identification of nucleic acid probes 20
12.6 serological methods 20
13 21 Test Report
Confirm microbial detection method 21 14
15 The results of quality assurance/quality control operations 21
15.1 Internal quality control 21
15.2 22 reference strains
15.3 External quality control (proficiency testing) 22
Appendix A (informative) Some disinfectant properties 23
Appendix B (normative) most probable number (MPN) determination 24
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard replaces SN 0330-1994 "export of food microbiological examination General."
This standard compared with SN 0330-1994, in addition to editorial changes outside the main technical changes are as follows.
--- Original standard name changed to "export Food Microbiology Inspection Law";
--- The scope of the original standard has been revised;
--- Added "laboratory spaces";
--- Added "operator";
--- Added "equipment";
--- Added "laboratory equipment preparation";
--- Increased "media preparation and sterilization";
--- Original standard "sample", "acceptance of the sample, sample preparation and storage of test" into the "laboratory samples" and press
ISO 7218 for the chapters have been revised;
--- Original standard "test" into "test", "Count", "qualitative detection method", "method validation" and other sections of the detailed description
He said, according to ISO 7218 has been revised chapters on content;
--- Original standard "inspection records and the results of the report" is replaced by "test report" and press the ISO 7218 chapters on the content
Amendment;
--- Added "Confirm microbiological testing methods";
--- Added "The results of quality assurance/quality control operations";
--- Deleting the original standard in Appendix A, increase the "most probable number (MPN) determination."
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard by the People's Republic of China Fujian Entry-Exit Inspection and Quarantine People's Republic of China, Inner Mongolia, China
People's Republic of Shanxi Entry-Exit Inspection and Quarantine of Jiangsu People's Republic of China, People's Republic of China Guangdong access
Habitat Inspection and Quarantine Bureau responsible for drafting.
The main drafters of this standard. Huang Xiaorong, Zheng Jing, Zheng Linyi, Liu middle school, Li Weihua, Jiang Yuan, Li Zhiyong.
This standard replaces the standards previously issued as follows.
--- SN 0330-1994.
Export Food Inspection General Microbiology
1 Scope
This standard specifies the general requirements for the implementation of food microbiology testing according to specific criteria.
This standard applies to food microbiology laboratory for food samples, food and food raw materials to produce environmental microbiology testing.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB 19489 General requirements for laboratory biosafety
GB/T 27025 testing and calibration laboratories General requirements
GB/T 27405 laboratory quality control standard microbiological testing of food
SN/T 1538.1 media preparation Guide - Part 1. General laboratory quality assurance prepared medium
SN/T 1538.2 media preparation Guide Part 2. A Practical Guide to medium performance test
SN/T 2102.1 foodborne pathogens PCR Detection Technology Specification - Part 1. General requirements and definitions
Between ISO /IEC Guide 43-1 than the use of the laboratory to verify the ability to test - Part 1. capacity-building and test plans
Implement
ISO 6887 for microbiological examination of food and animal feed microbiology and test samples initial suspension and decimal dilutions Preparation
ISO 8199 Quality microbial culture counts Guide
ISO 8261 Milk and milk products microbiological testing of samples and initial suspension and decimal dilutions Preparation General Guidelines
Within ISO 14461-1 Milk and milk quality control microbiological laboratories - Part 1. Analyst performance assessment for colony counts
Within ISO 14461-2 Milk and milk quality control microbiological laboratories - Part 2. Colony parallel plates and subsequent dilution steps
Count reliability determination
ISO 16140 Microbiology of food and animal feed alternative method validation procedures
ISO /T S19036 Microbiology of food and animal feed quantitative detection Measurement Uncertainty Evaluation Guide
3 laboratory spaces
3.1 Overview
The provisions are general requirements for laboratory design, pay attention to the reception and preparation of samples and other test samples shall be effectively isolated to prevent cross
Pollution.
3.2 Safety Requirements
Laboratories should be designed to comply with safety requirements for different types of microbes. Microorganisms into four hazard classes.
--- Hazard Class Ⅰ (low self-harm, low hazard groups). does not cause microbial human and animal diseases.
--- Hazard Class Ⅱ (moderate self-harm, harm low group). can cause human or animal disease, but generally for lab work
Person, group or environment will not cause serious harm to the pathogen. Laboratory exposure can cause serious infection, but effective treatment and
Preventive measures, and the risk of spread is limited.
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