SN/T 0184.4: Evolution and historical versions
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| SN/T 0184.4-2022 | English | RFQ |
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(Methods for the detection of Listeria monocytogenes in food for export - Part 4: Peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) method)
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SN/T 0184.4-2022
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| SN/T 0184.4-2010 | English | 279 |
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Detection of Listeria in foods. Part 4: Colloidal gold immunochromatographic method
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SN/T 0184.4-2010
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PDF similar to SN/T 0184.4-2022
Basic data | Standard ID | SN/T 0184.4-2022 (SN/T0184.4-2022) | | Description (Translated English) | (Methods for the detection of Listeria monocytogenes in food for export - Part 4: Peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) method) | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C53 | | Date of Issue | 2022-03-14 | | Date of Implementation | 2022-10-01 | | Issuing agency(ies) | General Administration of Customs | SN/T 0184.4-2010: Detection of Listeria in foods. Part 4: Colloidal gold immunochromatographic method
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Detection of Listeria in foods Part 4. Colloidal gold immunochromatographic method
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Listeria detected in foods
Part 4. colloidal gold
Part 4. Coloidalgoldimmunochromatographicmethod
Issued on. 2010-05-27
2010-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 0184 "Foods Listeria Detection" is divided into four parts.
--- Part 1. Food Importers monocytogenes Listeria testing methods;
--- Part 2. Multiplex PCR;
--- Part 3. Immunization beads;
--- Part 4. colloidal gold.
This section SN/T Section 40184 of.
This section drafted in accordance with GB/T 1.1-2009 given rules.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This part mainly drafted by. People's Republic of China Liaoning Province Exit Inspection and Quarantine.
The main drafters of this section. Wangqiu Yan, Cao Juan occasion, Zhao Xin, Cheng Chiu, Wang Gang, Xu Yang, Xu Junyi.
Listeria detected in foods
Part 4. colloidal gold
1 Scope
SN/T 0184 This section provides the food colloidal gold detection of Listeria.
This section applies to imported and exported food rapid screening tests Listeria.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member, undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB 19489 General requirements for laboratory biosafety
SN/T 0184.1 import and export food monocytogenes Listeria detection methods
Principle 3
This standard uses colloidal gold immunoassay technology, chloroauric acid reduction method with a certain diameter gold sol particles labeled Listeria
antibody. In nitrocellulose membrane as a carrier film is fixed in advance on the membrane containing the detection zone (T) of the anti-Listeria antibodies and controls
Anti-band (C) antibodies. When detected, the prepared sample is applied to the test strip sample well, the sample to another test strip by capillary action
Mobile terminal, the process of Listeria in the sample to form a complex with the first colloidal gold, and then specifically bind to the immobilized antibody on the membrane, and
Its interception and appear red.
4 Reagents and materials
Unless otherwise specified, all chemical reagents were of analytical grade, water should meet GB/T 6682 requirements.
4.1 Fraser broth enrichment broth (FB1, FB2). See Appendix A.
4.2 Reveal Listeria GICA rapid detection test strip 1).
1) This information is given for the convenience of users of this standard does not imply endorsement of the product. If other equivalent product has the same effect, then
You can use these equivalent products.
5. Apparatus
5.1 incubator. 30 ℃ ± 1 ℃, 24 ℃ ± 1 ℃, 35 ℃ ± 1 ℃.
5.2 homogenizer.
5.3 Balance. a sense of the amount of 0.1g.
5.4 Autoclave.
Sterile pipette 5.5, sterilized dishes sterile tubes.
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