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SN/T 0184.3-2008: Determination of Listeria monocytogenes in food for import and export. Part 3: Immuno-magnetic beads assay
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Determination of Listeria monocytogenes in food for import and export. Part 3: Immuno-magnetic beads assay
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SN/T 0184.3-2008
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Basic data | Standard ID | SN/T 0184.3-2008 (SN/T0184.3-2008) | | Description (Translated English) | Determination of Listeria monocytogenes in food for import and export. Part 3: Immuno-magnetic beads assay | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C53 | | Classification of International Standard | 07.100.30 | | Word Count Estimation | 10,171 | | Date of Issue | 2008-09-04 | | Date of Implementation | 2009-03-16 | | Quoted Standard | GB 19489; SN/T 0184.1 | | Regulation (derived from) | Industry standard filing Notice No. 11 of 2008 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export food Listeria monocytogenes bacteria sampling, sample preparation and immunomagnetic detection methods. This standard applies to import and export food Listeria monocytogenes bacteria test. | SN/T 0184.3-2008: Determination of Listeria monocytogenes in food for import and export. Part 3: Immuno-magnetic beads assay
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Determination of Listeria monocytogenes in food for import and export Part 3. Immuno-magnetic beads assay
Exit inspection and quarantine industry standard book People's Republic of China
Importers monocytogenes in foods
Listeria detection methods
Part 3. Immunization beads
Posted 2008-09-04
2009-03-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This section of the Appendix A, Appendix B are normative appendix.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section was drafted by. People's Republic of China Liaoning Province Exit Inspection and Quarantine.
The main drafters of this section. Wu Bin, Li Zhenrong, Huzhuan Wei, Wang Yuping, JIANG flag.
The first part of the Department of Inspection and Quarantine issued by industry standards.
Importers monocytogenes in foods
Listeria detection methods
Part 3. Immunization beads
1 Scope
SN/T 0184 provisions of this part of the import and export food monocytogenes Listeria sampling, sample preparation and IMB examination
method.
This section applies to imported and exported food monocytogenes Listeria test.
2 Normative references
The following documents contain provisions which, through reference SN/T 0184 and become part of the provisions of this section. For dated references,
All subsequent amendments (not including errata content) or revisions do not apply to this section, however, encourage the agreement on this section
Whether the parties can study the latest versions of these documents. For undated reference documents, the latest versions apply to this section.
GB 19489 General requirements for laboratory biosafety
SN/T 0184.1 import and export food monocytogenes Listeria testing methods
3 Methods Summary
The diameter of 0.05μm ~ 4μm magnetic microbeads Surface Chemical, and combined with the Listeria specific antibody, prepared
MACS, it can and Listeria in food antigen binding, so the detection of Listeria in food. The sample was 24h ~ 48h by
After the bacteria were taken 1mL enrichment broth is added and 20μL MACS lidded plastic tube, mixing in magnetic plate background, if you have listeria
Bacterial antigen is present, MACS will be captured, and the use of magnetic beads gather immune, chromogenic medium and inoculated into any after cleaning
Choose one medium (OXA or PALCAM agar) for the selective separation plates typical Listeria colonies for confirmation, final
Determine whether there monocytogenes Listeria through a series of tests.
4 equipment and appliances
4.1 Incubator. 37 ℃ ± 1 ℃; 41.5 ℃ ± 1 ℃.
4.2 Water bath. 45 ℃ ± 1 ℃.
4.3 Micropipettes. 20μL ~ 200μL, 10μL, 1mL.
4.4 Graduated pipettes. 5mL, 10mL.
4.5 Sterilization of glassware. cylinders, Erlenmeyer flasks, Petri dishes (diameter 90mm), test tubes.
4.6 sterile tube (Eppendorf tube). 1.5mL.
4.7 anti-Listeria immunity Beads. The beads can be obtained from commercial sources. It should be exactly in accordance with the manufacturer's instructions
operating.
4.8 vortex mixer.
4.9 with a magnetic carrier in a magnetic separator.
5 medium and reagents
Unless otherwise specified, the reagents were of analytical grade, water is distilled water.
5.1 pancreatic casein soy broth (TSB-YE). See Section A. Chapter 1.
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