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NY/T 727-2003: Determination of furazolidone in feeds. High performance liquid chromatography
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Determination of furazolidone in feeds. High performance liquid chromatography
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NY/T 727-2003
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Basic data | Standard ID | NY/T 727-2003 (NY/T727-2003) | | Description (Translated English) | Determination of furazolidone in feeds. High performance liquid chromatography | | Sector / Industry | Agriculture Industry Standard (Recommended) | | Classification of Chinese Standard | B20 | | Classification of International Standard | 65.120 | | Word Count Estimation | 10,197 | | Date of Issue | 2003-12-01 | | Date of Implementation | 2004-03-01 | | Quoted Standard | GB/T 6682; GB/T 14699.1 | | Adopted Standard | ISO 14797-1999 (E), MOD | | Summary | This standard provides high-performance liquid chromatography (HPLC) method for the determination of feed, concentrate feed and premix furazolidone approach. This standard can be used to contain 10mg/kg ~ 5000mg/kg furazolidone feed and content of 0. 5 % to 20 % of the pre-mixed feed and concentrated feed. | NY/T 727-2003: Determination of furazolidone in feeds. High performance liquid chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of furazolidone in feeds.High performance liquid chromatography
ICS 65.120
B20
People's Republic of China Agricultural Industry Standard
Determination of furazolidone in feed
High performance liquid chromatography
(ISO 14797, Animalfeedingstuffs-Determinationof
Chromatography, MOD)
Released on December 12,.2003
2004-03-01 implementation
Published by the Ministry of Agriculture
Foreword
This standard is modified to use ISO 14797.1999 (E) "High Performance Liquid Chromatography for the Determination of Furazolidone Content in Feeds" (English version).
This standard was redrafted in accordance with ISO 14797.1999(E).
In view of China's national conditions and laboratory equipment, this standard has been modified in the use of ISO 14797.1999 (E). Related skills
Surgical differences and editorial modifications have been incorporated into the text and identified by vertical single lines in the margins of the terms they refer to. In Appendix B
A list of these technical differences and editorial changes is given for reference.
Appendix A and Appendix B of this standard are informative annexes.
This standard was proposed by the Ministry of Agriculture of the People's Republic
This standard is under the jurisdiction of the National Feed Standardization Technical Committee.
This standard is drafted by. National Feed Product Quality Supervision and Inspection Center (Beijing), participated in drafting unit. Feed Industry of the Ministry of Agriculture
center.
The main drafters of this standard. Yan Huiwen, Yang Yuming, Zhao Xiaoyang, Wang Wei, Yang Wenjun.
Determination of furazolidone in feed
High performance liquid chromatography
1 Scope
This standard specifies the method for the determination of furazolidone in compound feed, premix feed and concentrated feed by high performance liquid chromatography (HPLC).
This standard can be used for compound feed containing 10mg/kg～5000mg/kg furazolidone and premixed feed with 0.5%-20% content.
Materials and concentrated feed.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study
Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 14699.1 Feed sampling method
3 Principle
The compound feed is moistened with a small amount of water, and the furazolidone is extracted with a mixture of acetonitrile and methanol, and the premixed feed and the concentrated feed are directly
The furazolidone is extracted with a mixture of acetonitrile and methanol, and the extract is purified by a short column of alumina and diluted with a diluent.
The phase was separated by HPLC and the UV detector was measured at 365 nm.
4 reagents and materials
Only reagents identified as superior or chromatographically pure were used in the analysis unless otherwise stated.
4.1 Water. Meet the requirements of GB/T 6682 first-class water.
4.2 Extractant. acetonitrile.methanol = 1.1. Mix well and put it at room temperature before use.
4.3 Diluent. Mix 350 mL of extractant (4.2) with 650 mL of water (4.1).
4.4 10% acetic acid solution. Dilute 10 mL of glacial acetic acid to 100 mL with water.
4.5 Sodium acetate buffer, hydrazine (CH3CO2Na) = 0.01 mol/L, pH = 6.0.
Dissolve 0.82 g of sodium acetate with about 700 mL of water, adjust the pH to 6.0 with acetic acid solution (4.4), and dilute to 1000 mL with water.
Mix well.
4.6 HPLC mobile phase. Take 800 mL of sodium acetate buffer (4.5) and.200 mL of acetonitrile, mix well and pass through a 0.2 μm filter.
Filtration, ultrasonic degassing for 10 min before use.
4.7 Furazolidone standard. N-(5-nitro-2-furomethylidene)-3-amino-2-oxazolidinone.
Note. Since furazolidone is very sensitive to light, all operations should be carried out in the dark, and should be avoided during inhalation and contact.
Toxic furazolidone standards and solutions, the preparation of the solution should be carried out in a fume hood, work with glasses, wear overalls protection.
4.8 furazolidone stock solution (about 250μg/mL). weigh 25mg ± 1mg furazolidone standard (4.7), accurate to 0.1mg, with
The solution (4.2) was dissolved, diluted to 100 mL, mixed, and stored in a refrigerator at 0 ° C to 8 ° C. The purity of the standard is taken into account when calculating the solution concentration.
The solution is valid for one month.
4.9 furazolidone working solution (about 5μg/mL and 12.5μg/mL). accurately absorb 2.0mL and 5.0mL stock solution (4.8) separately
In a 100 mL volumetric flask, add 65 mL of water, dilute to the mark with the extract (4.2) and mix well. Prepare fresh work for each batch of samples.
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