NY/T 565: Evolution and historical versions
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| NY/T 565-2025 | English | RFQ |
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(Medi - Diagnostic technology for Vesna disease)
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NY/T 565-2025
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| NY/T 565-2002 | English | 199 |
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Agar gel immunodiffusion test for maedi-visna disease
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NY/T 565-2002
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Basic data | Standard ID | NY/T 565-2025 (NY/T565-2025) | | Description (Translated English) | (Medi - Diagnostic technology for Vesna disease) | | Sector / Industry | Agriculture Industry Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Date of Issue | 2025-01-09 | | Date of Implementation | 2025-05-01 | | Issuing agency(ies) | Ministry of Agriculture and Rural Affairs | NY/T 565-2002: Agar gel immunodiffusion test for maedi-visna disease---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Agar gel immunodiffusion test for maedi-visna disease
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People's Republic of China Agricultural Industry Standard
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Medicinal Wisner disease agar gel immunodiffusion
experiment method
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Implementation
Published by the Ministry of Agriculture
Foreword
Maedi-Visna disease is a chronic fatal infection of sheep, World Organization for Animal Health [WorldOrganization]
forAnimalHealth (English), OfficeIntentionaldesEpizootic (French), OIE] listed as Class B, China listed it as Class II
Animal disease.
Visna-Maedi, also known as sheep progressive pneumonia, progressive interstitial pneumonia, etc., is clinically and pathologically
Two different chronic progressive infectious diseases. The incubation period of these two diseases is several months to several years, the course of disease is long, and the development is slow.
And ended up. Medi disease is characterized by chronic progressive interstitial pneumonia. The diseased sheep is thin, breathing difficulties, dry cough, and finally death, the flock is dead.
The rate is 20% to 30%. Wisner's disease is characterized by chronic demyelinating subacute encephalitis. The diseased sheep trembles, the head posture is abnormal, and the hind limbs are numb.
痹, then developed into paraplegia, generalized paralysis, flock mortality rate of 6% to 10%. Despite the clinical symptoms and diseases of Medy's disease and Wisner's disease
There are big differences in the changes, but virology, serology, molecular biology and animal experiments have proved that the pathogens of the two diseases are the same disease.
Poison, the Medi-Wisner virus. In 1923, the disease was first discovered in the Canadian Montana sheep, and in Iceland in 1933.
OK, after which Wisner's disease was discovered. At present, the disease mainly occurs or has occurred in Iceland, Germany, France, the Netherlands, the United States, South Africa, Kenya, India.
Degree, Israel, Peru and other countries and regions.
Suspicious cases have also been found in China in the 1960s, and their clinical symptoms and necropsy changes are similar to those of Medica. From Australia and New in 1984
The Leyster sheep and their offspring in the border area introduced by Xilan detected antibodies and isolated the virus in 1985. The disease has no vaccine for prevention.
There is no effective treatment method. Once the flock is infected with the disease, the production performance is degraded and eventually dying, causing huge losses to the sheep industry.
This standard is based on the OIE Diagnostic Test and Biological Reagent Standards Manual (2000 Edition) (ManualofStandardsforDiag-
nosticTestsandVaccines,.2000) "Agar immunodiffusion test (International Trade Designation Test)" and this section as defined in Chapter 2.4.4/5
Years of experimental research and practice in the country, but the following modifications were made. the pore size and pore spacing of the test were slightly different, and did not affect the test results.
Easier to operate and observe.
Appendix A of this standard is a normative appendix.
This standard was proposed by the Ministry of Agriculture, Animal Husbandry and Veterinary Bureau.
This standard is under the jurisdiction of the National Animal Quarantine Standardization Technical Committee.
This standard was drafted. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences.
The main drafters of this standard. Rong Jun Gong, Wei Renshan.
Medi-Visner disease agar gel immunodiffusion
experiment method
1 Scope
This standard specifies the technical requirements for the Medi-Visner disease agar gel immunodiffusion test.
This standard applies to the detection, diagnosis and epidemiological investigation of the infection of Medi-Visner's disease.
2 agar immunogel diffusion test
2.1 Material preparation
2.1.1 Agar plates, the preparation method is shown in Appendix A (normative appendix).
2.1.2 antigen, standard positive serum, negative serum.
2.2 Method of operation
2.2.1 Agar plates, preparation and punching are shown in Appendix A.
2.2.2 Pipette the antigen and add the center hole until it is full.
2.2.3 Draw the standard positive serum and add 2, 4, and 6 holes in the periphery, and fill up. Each test should include a weak positive control.
2.2.4 Drain the test serum and add the outer 1, 3, and 5 holes, and fill up.
2.3 Determination
2.3.1 After the antigen, the standard positive serum and the test serum are added, the plate is inverted, and the humidification box is incubated at 37 ° C for 24 h.
48h to observe whether there is a sedimentation line.
2.3.2 There is a clear sedimentation line between the standard positive serum and the antigenic well, and there is also a distinct sedimentation line between the tested serum and the antigenic well, and the standard
The precipitation line of the positive serum is fused, or the end of the precipitation line of the standard positive serum and the antigen hole is clearly at the center of the adjacent test serum hole.
When the hole direction is bent, the test serum is positive (+).
When there is a clear sedimentation line between the standard positive serum and the antigen hole, there is no precipitation line between the test serum and the antigen hole, or the standard positive serum and anti-antibody
When the end of the original inter-well sedimentation line extends straight to or toward the adjacent test serum hole, the test serum is negative (-).
It is suspicious (±) between yin and positive.
Suspicious should be re-examined and still suspected to be positive (+).
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