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NY/T 550-2002: Defection methods of salmonella for animal and animal products
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| NY/T 550-2002 | English | 229 |
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Defection methods of salmonella for animal and animal products
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NY/T 550-2002
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Basic data | Standard ID | NY/T 550-2002 (NY/T550-2002) | | Description (Translated English) | Defection methods of salmonella for animal and animal products | | Sector / Industry | Agriculture Industry Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 7,721 | | Date of Issue | 2002-08-27 | | Date of Implementation | 2002-12-01 | | Quoted Standard | GB 4789.4-1994; GB 4789.28-1994 | | Summary | This standard specifies the animals and animal products salmonella detection methods. This standard applies to animals and animal products, salmonella testing. | NY/T 550-2002: Defection methods of salmonella for animal and animal products---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Defection methods of salmonella for animal and animal products
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People's Republic of China Agricultural Industry Standard
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Method for detecting Salmonella in animal and animal products
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Implementation
Published by the Ministry of Agriculture
Foreword
Salmonella is an important zoonotic pathogen, which is also an important pathogen causing food poisoning. It has important public health.
Meaning of life.
The biochemical screening and signing of this standard were carried out simultaneously, and the serum agglutination test directly used the fresh lawn moss on the slant of the trisaccharide iron agar as the antigen.
This standard was proposed by the Ministry of Agriculture, Animal Husbandry and Veterinary Bureau.
This standard is under the jurisdiction of the National Animal Quarantine Standardization Technical Committee.
This standard was drafted. Xiamen Entry and Exit Inspection and Quarantine Bureau, Fujian Provincial Health and Epidemic Prevention Station.
The main drafters of this standard. Li Biping, Sun Fuquan, Xie Yijun, Lin Bingling.
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Method for detecting Salmonella in animal and animal products
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This standard specifies the detection method for Salmonella in animal and animal products.
This standard applies to the detection of Salmonella in animal and animal products.
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, all subsequent
Modifications (not including errata content) or revisions do not apply to this standard, however, research that encourages agreements based on this standard is
No, the latest version of these files can be used. For undated references, the latest edition applies to this standard.
GB 4789.4-1994 Food hygiene microbiological examination Salmonella test
GB 4789.28-1994 Food hygiene microbiological examination Staining method, medium and reagents
Detection method
. Equipment
Balances, homogenizers, mortars, incubators, jars, test tubes, straws, plates, glass rods.
. Medium and reagents
.. Buffer Protein Hydrophobic (BP). For the preparation method, see 4.12 of GB 4789.28-1994.
.. Magnesium chloride malachite green enrichment liquid (MM). For the preparation method, see 4.13 of GB 4789.28-1994.
.. Sodium tetrasulfonate brilliant green enrichment liquid (TTB). For the preparation method, see 4.14, 4.15 of GB 4789.28-1994.
.. Selenite cystine enrichment solution (SC). For the preparation method, see 4.16 of GB 4789.28-1994.
.. Deoxycholatehyadrogensulfialelactoseagar (DHL). For the preparation method, see GB 4789.28-
4.20 in.1994.
.. Hematoxylin (HE) agar (hektoenentericagar). For the preparation method, see 4.21 of GB 4789.28-1994.
.. WS agar. For the preparation method, see 4.23 of GB 4789.28-1994.
.. Trisaccharide Iron Agar (TSI). For the preparation method, see 4.26 or 4.27 of GB 4789.28-1994.
.. Amino acid decarboxylase test medium. For the preparation method, see 3.12 of GB 4789.28-1994.
.. O-nitrophenol U-爟-galactoside (ONPG) medium. For the preparation method, see 3.3 of GB 4789.28-1994.
.. Mannitol Sugar Fermentation Tube Medium (MAN). For the preparation method, see 3.2 of GB 4789.28-1994.
.. Salmonella factor serum. 26 for preliminary typing. 163 for detailed typing.
. Sampling and preparation of samples
.. Samples are collected according to the test or sampled according to relevant regulations.
.. Preparation of sample. frozen sample, solid sample need to use a homogenizer to 8000r stew min ~ 10000r stew min min break 1min, or with milk
钵 Grinding to make a sample. liquid sample, powder sample is mixed with a sterile glass rod.
. Test procedure
The Salmonella test procedure is shown in Figure 1.
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