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LY/T 2745-2016 English PDF

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LY/T 2745-2016: Identification of kernel-using apricot cultivars. SSR marker method
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LY/T 2745-2016	English	329	Add to Cart	3 days [Need to translate]	Identification of kernel-using apricot cultivars. SSR marker method	Valid	LY/T 2745-2016

PDF similar to LY/T 2745-2016

Standard similar to LY/T 2745-2016

GB/T 29371.2 GB/T 29371.1 GB 4404.4

Basic data

Standard ID	LY/T 2745-2016 (LY/T2745-2016)
Description (Translated English)	Identification of kernel-using apricot cultivars. SSR marker method
Sector / Industry	Forestry Industry Standard (Recommended)
Classification of Chinese Standard	B21
Classification of International Standard	67.060
Word Count Estimation	14,111
Date of Issue	2016-10-19
Date of Implementation	2017-01-01
Regulation (derived from)	State Forestry Administration Announcement No.1916 of 2016; Industry Standard Announcement No.16 of 2016 (No.204)
Issuing agency(ies)	State Forestry Administration

LY/T 2745-2016: Identification of kernel-using apricot cultivars. SSR marker method

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(Standard Specification for Identification of Apricot Varieties - SSR Molecular Marker) ICS 67.060 B21 People's Republic of China Forestry Industry Standard Technical specification for identification of apricot varieties SSR molecular marker Released on October 18,.2016 2017-01-01 implementation State Forestry Administration released

Content

Foreword I 1 Scope 1 2 Terms and Definitions 1 3 Principle 1 4 instruments and main reagents, consumables 2 5 solution configuration 2 6 core primer 2 7 Operation 2 8 fingerprint comparison 3 9 result judgment 4 Appendix A (informative) Instruments and main reagents, consumables 5 Appendix B (informative) Solution configuration 6 Appendix C (Normative) Core Primer 8 Appendix D (informative appendix) SSR fingerprint identification report for apricot varieties Reference 11

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the Chinese Academy of Forestry. This standard is under the jurisdiction of the State Forestry Administration. This standard was drafted. Economic Forestry Research and Development Center of China Academy of Forestry, Beijing Forestry University. The main drafters of this standard. Fu Dali, Liu Mengpei, Qin Yu, Zhu Gaopu, Zhao Han. Technical specification for identification of apricot varieties SSR molecular marker

1 Scope

This standard specifies the use of simple repeat sequences (SSR) molecular markers to identify apricots (Armeniacacathayana D.L. Fuetal.) technical specifications of the variety. This standard applies to the identification of SSR molecular fingerprints of apricot varieties.

2 Terms and definitions

The following terms and definitions apply to this document. 2.1 Test species testcultivar The variety of apricots to be identified shall be provided by the examiner. 2.2 Control variety comparingcultivar For the varieties that are used for fingerprint comparison with the varieties to be inspected (the varieties to be inspected are new varieties, the control varieties are similar varieties; the varieties to be inspected For counterfeit varieties, the control variety is a true variety), which is provided by the examiner or others. 2.3 Core primer coreprimer The amplified product has high stability and consistency (no interference band) and has strong discriminating ability, which can be used to establish apricot Synthetic primers for fingerprint alignment. 2.4 Fingerprint SSRfingerprint DNA fragments of the tested and control varieties amplified by SSR core primers (or equivalent to core primers), passed through polypropylene Amide electrophoresis or capillary electrophoresis to obtain maps of DNA fragments of different sizes. 2.5 Fingerprint comparison SSRfingerprinting Comparing the SSR fingerprints of the tested varieties with the control varieties, and analyzing whether the SSR fingerprints of the tested and control varieties have There are differences and their size is determined.

3 Principle

Simple repeat sequence molecular markers (SSRs), often referred to as "microsatellite markers," are distributed at different locations throughout the genome of apricot kernels. The number of repeating units at each locus of different varieties may be different, thus forming a polymorphism of the SSR marker. Due to each simple repeat The sequences at both ends are highly conserved single-copy sequences, so specific primers can be designed based on the sequences at both ends, using PCR to The DNA repeats between the primers are amplified, and the amplified product fragments of different sizes are separated by electrophoresis and labeled with fluorescent dyes. distinguish. Therefore, according to the polymorphism of the SSR locus, the apricot variety can be identified by PCR amplification and electrophoresis.

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