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LST6145-2023: Inspection of grain and oils--Detection of lead in grains--Rapid quantitative method of colloidal gold technique
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| LS/T 6145-2023 | English | 179 |
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Inspection of grain and oils--Detection of lead in grains--Rapid quantitative method of colloidal gold technique
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LS/T 6145-2023
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Basic data | Standard ID | LS/T 6145-2023 (LS/T6145-2023) | | Description (Translated English) | Inspection of grain and oils--Detection of lead in grains--Rapid quantitative method of colloidal gold technique | | Sector / Industry | Food & Beverage Industry Standard (Recommended) | | Classification of Chinese Standard | B20 | | Classification of International Standard | 67.060 | | Word Count Estimation | 7,743 | | Date of Issue | 2023-03-20 | | Date of Implementation | 2023-09-20 | | Issuing agency(ies) | National Grain and Material Reserves Administration | LST6145-2023: Inspection of grain and oils--Detection of lead in grains--Rapid quantitative method of colloidal gold technique
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 67:060
CCSB20
People's Republic of China Grain Industry Standards
Determination of Lead in Grain and Oil Inspection
Colloidal gold rapid quantification method
Released on 2023-03-20
2023-09-20 Implementation
Released by the State Administration of Grain and Material Reserves
Published by China Standard Press
foreword
This document is in accordance with the provisions of GB/T 1:1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules for Standardization Documents"
drafting:
Please note that some contents of this document may refer to patents: The issuing agency of this document assumes no responsibility for identifying patents:
This document is proposed by the State Administration of Grain and Material Reserves:
This document is under the jurisdiction of the National Grain and Oil Standardization Technical Committee (SAC/TC270):
This document was drafted by: Nanjing University of Finance and Economics, National Grain and Material Reserve Bureau Scientific Research Institute, Beijing Grain, Oil and Food Inspection Institute, Beijing
Huaan Maike Biotechnology Co:, Ltd:, Shandong Meizheng Biotechnology Co:, Ltd:, Jiangsu Meizheng Biotechnology Co:, Ltd:, Heilongjiang Foodstuffs
Quality and Safety Monitoring Center, Anhui Provincial Grain and Oil Product Quality Supervision and Inspection Station, Jilin China Grain Grain and Oil Quality Supervision Center Co:, Ltd:, Liaoning Provincial Inspection
Testing and Certification Center, Guangxi Zhuang Autonomous Region Grain and Oil Quality Inspection Center, Heilongjiang China Grain Quality Supervision Center Co:, Ltd:, Liaoning China Grain Grain and Oil
Quality Supervision Center Co:, Ltd:, Sinograin Chengdu Storage Research Institute Co:, Ltd:, Shanxi Grain Quality Monitoring Center, Hebei Province Grain and Oil Quality Inspection
center:
The main drafters of this document: Yuan Jian, Xing Changrui, Guo Jian, Zhao Xiaoxu, Zhang Junchao, Mao Yongrong, Wang Songxue, Liu Meichen, Zhou Minghui, Ji Lanyang,
Xu Chunfeng, Du Dongxin, Zhu Xudong, Huang Dong, Zhao Min, Ji Libo, Xiong Shengwei, Yu Yin, Tan Junfeng, Wang Lei:
Determination of Lead in Grain and Oil Inspection
Colloidal gold rapid quantification method
1 Scope
This document describes the principle of the colloidal gold rapid quantitative method for the determination of lead content, and specifies the reagents and materials, equipment, samples, and determination procedures:
with the measurement results:
This document is applicable to the rapid determination of lead content in paddy, brown rice, rice, wheat and corn:
2 Normative references
The contents of the following documents constitute the essential provisions of this document through normative references in the text: Among them, dated references
For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
this document:
GB 5009:12 National Food Safety Standard Determination of Lead in Food
GB/T 5491 Grain and Oil Seed Inspection Sampling and Sampling Method
GB/T 6682 Analytical laboratory water specifications and test methods
3 principles
After the lead in the sample is extracted by the hydrochloric acid solution, it is chelated with a chelating agent, and combined with the specific antibody labeled with the colloidal gold solution, which inhibits the chromatographic
During the process, the immune reaction between the antibody and the lead-bovine serum albumin conjugate on the nitrocellulose membrane detection line (T line) makes the T signal value on the detection line absorb
The luminosity decreases, and the content of lead in the sample can be obtained through the change of absorbance between the T signal value of the detection line and the C signal value of the quality control line (C line):
4 Reagents and materials
4:1 Water: GB/T 6682, Class II:
4:2 Hydrochloric acid solution: Weigh 58:40g of sodium chloride, add 900mL of water to dissolve, add 33:3mL of concentrated hydrochloric acid (12mol/L), and dilute to volume with water
to 1L:
4:3 Phosphate buffer: weigh 0:34g sodium dihydrogen phosphate, 51:60g disodium hydrogen phosphate, 8:50g sodium chloride, add 900mL water to dissolve,
Make up to 1L with water:
4:4 8% EDTA disodium solution: Weigh 8:00g EDTA disodium in a 100mL volumetric flask, dissolve in water and make to volume
to scale:
4:5 Heavy metal lead rapid quantitative detection kit: contains gold-labeled microwells, test strips, and detection reagents (4:2, 4:3, 4:4), which need to be refrigerated (2°C~
8°C) for storage, the technical requirements shall comply with the provisions of Appendix A:
5 Instruments and equipment
5:1 Pulverizer: After the sample is pulverized, it can pass through a 0:85mm sieve:
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