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LST6143-2023: Inspection of grain and oils--Determination of aflatoxin B1 in cereals--Time-resolved fluorescent immunochromatographic method
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| LS/T 6143-2023 | English | 199 |
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Inspection of grain and oils--Determination of aflatoxin B1 in cereals--Time-resolved fluorescent immunochromatographic method
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LS/T 6143-2023
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Basic data | Standard ID | LS/T 6143-2023 (LS/T6143-2023) | | Description (Translated English) | Inspection of grain and oils--Determination of aflatoxin B1 in cereals--Time-resolved fluorescent immunochromatographic method | | Sector / Industry | Food & Beverage Industry Standard (Recommended) | | Classification of Chinese Standard | X11 | | Classification of International Standard | 67.040 | | Word Count Estimation | 8,855 | | Date of Issue | 2023-03-20 | | Date of Implementation | 2023-09-20 | | Issuing agency(ies) | National Grain and Material Reserves Administration | LST6143-2023: Inspection of grain and oils--Determination of aflatoxin B1 in cereals--Time-resolved fluorescent immunochromatographic method
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 67:040
CCSX11
People's Republic of China Grain Industry Standards
Determination of Aflatoxin B1 in Cereals and Oils
time-resolved fluorescence immunochromatographic quantification
Released on 2023-03-20
2023-09-20 Implementation
Released by the State Administration of Grain and Material Reserves
Published by China Standard Press
foreword
This document is in accordance with the provisions of GB/T 1:1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules for Standardization Documents"
drafting:
Please note that some contents of this document may refer to patents: The issuing agency of this document assumes no responsibility for identifying patents:
This document is proposed by the State Administration of Grain and Material Reserves:
This document is under the jurisdiction of the National Grain and Oil Standardization Technical Committee (SAC/TC270):
This document was drafted by: National Institute of Grain and Material Reserves, Institute of Oil Crops, Chinese Academy of Agricultural Sciences, Beijing Zhiyun
Da Technology Co:, Ltd:, Jiangnan University, Sinograin Chengdu Storage Research Institute Co:, Ltd:, Henan Food and Salt Industry Inspection Technology Research Institute,
Suining Grain Quality Supervision and Inspection Station, Guangxi Zhuang Autonomous Region Grain and Oil Quality Inspection Center, Beijing Grain, Oil and Food Inspection Institute, Fujian Grain and Oil
Quality Monitoring Institute, Hebei Provincial Grain and Oil Quality Inspection Center, Inner Mongolia Autonomous Region Grain and Oil Quality Inspection Center, Beijing Academy of Agriculture and Forestry Sciences:
The main drafters of this document: Ye Jin, Wang Songxue, Zhang Bing, Yu Li, Sun Xiulan, Lan Shengbin, Chen Jinying, Wang Wenjun, Ji Jiansheng, Li Xiaoming,
Wu Xianshao, Yang Hongwei, Huang Jianli, Yi Weimin, Qiu Qingfeng, Wang Meng, Sang Huachun:
Determination of Aflatoxin B1 in Cereals and Oils
time-resolved fluorescence immunochromatographic quantification
1 Scope
This document specifies the principle, reagents, materials and instruments for the quantitative determination of aflatoxin B1 in grains by time-resolved fluorescent immunochromatographic method
and equipment, sample preparation, sample determination, presentation of results, and precision:
This document applies to the rapid detection of aflatoxin B1 in cereals:
The detection limit of the method in this document is 0:7μg/kg, and the quantification limit is 2:0μg/kg:
2 Normative references
The contents of the following documents constitute the essential provisions of this document through normative references in the text: Among them, dated references
For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
this document:
GB 2761 National Food Safety Standard Limits of Mycotoxins in Food
GB 5009:22-2016 National Food Safety Standard Determination of Aflatoxin Group B and Group G in Food
GB/T 5491 Grain and Oil Seed Inspection Sampling and Sampling Method
GB/T 6682 Analytical laboratory water specifications and test methods
3 principles
After the aflatoxin B1 in the sample binds to the specific antibody labeled with polystyrene microspheres containing europium-chelates, it inhibits the chromatographic
During the process, the immune reaction between the antibody and the aflatoxin B1-BSA conjugate on the nitrocellulose membrane detection line reduces the fluorescence intensity on the detection line,
The content of aflatoxin B1 in the sample is automatically calculated by the change of fluorescence intensity and the built-in curve of the fluorescence analyzer:
4 Reagents and materials
Unless otherwise specified, the reagents used are of analytical grade, and the water used in the laboratory shall meet the requirements for third-grade water in GB/T 6682:
4:1 Methanol
4:2 Methanol solution: Take 70mL of methanol and add 30mL of water, mix well:
4:3 Sample diluent: provided with the product, or prepared according to the product manual:
4:4 Aflatoxin B1 time-resolved fluorescence immunochromatography card (hereinafter referred to as "immunochromatography card"): the performance of the immunochromatography card has been determined to be
Comply with the provisions of Appendix A, and refer to the instructions for use for specific storage conditions:
5 Instruments and equipment
5:1 Balance: Sensitivity 0:01g:
5:2 Pulverizer: The motor speed is ≥1000r/min, so that all the samples can pass through the 0:85mm sieve after pulverization:
5:3 Centrifuge: speed ≥ 3000r/min:
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