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LST6122-2017: Inspection of grain and oils - Determination aflatoxin content in grains and oils - Cleanup by immunoaffinity chromatography and determination by high-performance liquid chromatography and post-column photochemical derivatization
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| LS/T 6122-2017 | English | 229 |
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Inspection of grain and oils - Determination aflatoxin content in grains and oils - Cleanup by immunoaffinity chromatography and determination by high-performance liquid chromatography and post-column photochemical derivatization
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LS/T 6122-2017
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Basic data | Standard ID | LS/T 6122-2017 (LS/T6122-2017) | | Description (Translated English) | Inspection of grain and oils - Determination aflatoxin content in grains and oils - Cleanup by immunoaffinity chromatography and determination by high-performance liquid chromatography and post-column photochemical derivatization | | Sector / Industry | Food & Beverage Industry Standard (Recommended) | | Classification of Chinese Standard | X11 | | Classification of International Standard | 67.060 | | Word Count Estimation | 10,162 | | Date of Issue | 2017-10-27 | | Date of Implementation | 2017-12-20 | | Issuing agency(ies) | National Grain Administration | LST6122-2017: Inspection of grain and oils - Determination aflatoxin content in grains and oils - Cleanup by immunoaffinity chromatography and determination by high-performance liquid chromatography and post-column photochemical derivatization
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Inspection of grain and oils-Determination aflatoxin content in grains and oils-Cleanup by immunoaffinity chromatography and determination by high-performance liquid chromatography and post-column photochemical derivatization
ICS 67.060
X11
People's Republic of China food industry standard
Grain and oil testing aflatoxin content in grain, oil and products
Post-column photochemical derivatization high performance liquid chromatography
Released on.2017-10-27
2017-12-20 implementation
National Food Bureau released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed by the National Grain Administration.
This standard is under the jurisdiction of the National Grain and Oil Standardization Technical Committee (SAT/TC270).
This standard was drafted. National Academy of Food Science Research Institute, Beijing Cereals, Oils and Foodstuffs Inspection Institute, Hubei Province Cereals, Oils and Foodstuffs Quality Monitoring Station, North
Beijing Zhongwei Weikang Technology Co., Ltd., Beijing Agricultural Quality Standards and Testing Technology Research Center.
The main drafters of this standard. Wang Songxue, Wang Xiong, Qi Qin, Xiong Ning, Xie Gang, Li Rui, Zhang Yan, Zhang Rui, Zhou Minghui, Lu Anxiang.
Grain and oil testing aflatoxin content in grain, oil and products
Post-column photochemical derivatization high performance liquid chromatography
1 Scope
This standard specifies the principle of post-column photochemical derivatization high performance liquid chromatography for the determination of aflatoxin content in food and edible vegetable oils.
Reagents and materials, instruments and equipment, sample preparation, sample preparation, procedure, calculation of results and precision.
This standard applies to the determination of aflatoxin content in grain, oil and products (except oil).
The quantitative detection limits of aflatoxins B1, B2, G1 and G2 in this standard are 0.5μg/kg, 0.25μg/kg, 1.0μg/kg and
0.5 μg/kg.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 5491 Inspection of food and oil materials, sample method
GB/T 5524 animal and vegetable oils
GB/T 6682 Analytical laboratory water specifications and test methods
Preparation of GB/T 15687 animal and vegetable oil and fat samples
3 Principle
The sample is extracted by methanol-water, and the extract is purified by filtration, dilution and immunoaffinity chromatography, and separated by high performance liquid chromatography.
The photochemical derivatizer after the column is used to derivatize the isolate to generate a fluorescent signal, and the fluorescence intensity is measured by a fluorescence detector.
The content of aflatoxin in the sample was calculated by the standard curve method.
4 reagents and materials
Unless otherwise stated, all reagents are of analytical grade and water should meet the requirements of Class I water in GB/T 6682.
4.1 Acetonitrile. chromatographically pure.
4.2 Methanol. chromatographically pure.
4.3 Sodium chloride (NaCl).
4.4 Methanol water (7 3). Take 70 mL of methanol (4.2) plus 30 mL of water.
4.5 Methanol acetonitrile water (35 10 55). Take 35 mL of methanol (4.2) and 10 mL of acetonitrile (4.1) plus 55 mL of water.
4.6 Aflatoxin standards (aflatoxins B1, B2, G1, G2). purity ≥ 99%.
4.7 Aflatoxin standard stock solution. accurately weigh the appropriate amount of aflatoxin standard (4.6), using acetonitrile (4.1) or methanol (4.2) respectively
2μg/mL standard stock solution of aflatoxin B1, B2, G1, G2 was prepared and stored at 4 °C for 3 months.
Note. Considering that aflatoxin is carcinogenic, there are many uncertainties in the safety and quantity protection of the standard stock solution prepared with powder. It is recommended to directly
Purchase a commercial aflatoxin standard solution.
4.8 Aflatoxin mixed standard working solution. accurately transfer appropriate amount of aflatoxin B1, B2, G1, G2 standard stock solution (4.7),
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