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LST6109-2014: Inspection of grain and oils. Detection of zearalenone in cereal. Rapid method of colloidal gold technique
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| LS/T 6109-2014 | English | 189 |
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Inspection of grain and oils. Detection of zearalenone in cereal. Rapid method of colloidal gold technique
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LS/T 6109-2014
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Basic data | Standard ID | LS/T 6109-2014 (LS/T6109-2014) | | Description (Translated English) | Inspection of grain and oils. Detection of zearalenone in cereal. Rapid method of colloidal gold technique | | Sector / Industry | Food & Beverage Industry Standard (Recommended) | | Classification of Chinese Standard | B20 | | Classification of International Standard | 67.060 | | Word Count Estimation | 7,749 | | Date of Issue | 5/5/2014 | | Date of Implementation | 6/1/2014 | | Quoted Standard | GB 2761; GB 5491; GB/T 6682-2008; GB/T 23504 | | Regulation (derived from) | National Food Authority Circular (2014 No. 3) | | Issuing agency(ies) | National Grain Administration | | Summary | This Standard specifies the zearalenone colloidal gold rapid test card detection method principle, reagents and materials, instruments and equipment, sample preparation, sample side fixed, and determines that the positive samples were confirmed. This Stan | LST6109-2014: Inspection of grain and oils. Detection of zearalenone in cereal. Rapid method of colloidal gold technique
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Inspection of grain and oils.Detection of zearalenone in cereal.Rapid method of colloidal gold technique
ICS 67.060
B20
Record number. 44930-2014
People's Republic of China food industry standard
Determination of zearalenone in grains tested by grain and oil
Colloidal gold quick test card method
Published on.2014-04-28
2014-06-01 implementation
National Food Bureau released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is under the jurisdiction of the National Grain and Oil Standardization Technical Committee (SAC/TC270).
This standard was drafted. Beijing Grain and Oil Food Inspection Institute, Hubei Grain and Oil Food Quality Monitoring Station, Sichuan Grain and Oil Center Monitoring Station, Liao
Ning Province Cereals and Oils Inspection and Monitoring Institute, Chongqing Grain and Oil Quality Supervision and Inspection Station, Guangxi Zhuang Autonomous Region Grain and Oil Quality Supervision and Inspection Station, National Grain Bureau
Standard Quality Center, Shangqiu City Grain and Oil Technology Inspection Institute, Henan Province.
The main drafters of this standard. Guo Jian, Wang Zhengping, Liu Jian, Yang Jun, Zhu Xudong, Peng Chao, Liu Yongying, Liu Zheng, Zhang Yan, Yang Weimin, Shao Hui.
Determination of zearalenone in grains tested by grain and oil
Colloidal gold quick test card method
1 Scope
This standard specifies the principle, reagents and materials, instruments and equipment, and samples of the colloidal gold rapid test card detection method for zearalenone.
Preparation, sample determination, result determination, positive sample confirmation.
This standard applies to the rapid screening of zearalenone in cereals such as wheat and corn.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB 2761 National Standard for Food Safety, Limits of Mycotoxins in Foods
GB 5491 Inspection of food and oil materials, sample method
GB/T 6682-2008 Analytical laboratory water specifications and test methods
GB/T 23504 Determination of Zearalenone in Foods by Immunoaffinity Chromatography Purification High Performance Liquid Chromatography
3 Principle
The method is based on antigen-antibody reaction, immunogold labeling technology and capillary chromatography technology, and the microporous membrane is used as a solid phase carrier, which can be specific
The antibody identifying zearalenone is labeled as red or purple-red colloidal gold particles, and the two bands on the membrane are fixed to fix the corn red
The mycophenolate antigen and the secondary antibody against the murine antibody are determined based on the color change of the ribbon.
4 reagents and materials
Unless otherwise stated, the reagents used are of analytical grade, and the laboratory water should meet the third grade water in GB/T 6682-2008.
4.1 Zearalenone Colloidal Gold Test Card 1). The performance of the test card should meet the requirements of Appendix A. Testing in different batches of different brands
Before using the card, test card performance shall be determined in accordance with Appendix A.
1) Deoxygenated snow rot produced by Beijing Huaan Maike Biotechnology Co., Ltd., Shanghai Fasting Biotechnology Co., Ltd. and Nanchang Boheng Biotechnology Co., Ltd.
The Fusarium Enol Colloidal Gold Test Card is a fast-testing card that is widely used today. This information is provided solely for the convenience of users of this standard and does not represent
This standard recognizes the product. Any test card that meets the requirements of Appendix A of this standard can be used.
4.2 Extract. Special extract of 70% methanol solution or colloidal gold test card.
5 instruments and equipment
In addition to the laboratory's conventional equipment, the following equipment should be noted.
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