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JJF 2056-2023 English PDF

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JJF 2056-2023: Calibration Specification for Automatic Microbial Detection Systems based on Impedance Method
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Basic data

Standard ID JJF 2056-2023 (JJF2056-2023)
Description (Translated English) Calibration Specification for Automatic Microbial Detection Systems based on Impedance Method
Sector / Industry Metrology & Measurement Industry Standard
Word Count Estimation 17,161
Date of Issue 2023-06-30
Date of Implementation 2023-12-30
Issuing agency(ies) State Administration for Market Regulation

JJF 2056-2023: Calibration Specification for Automatic Microbial Detection Systems based on Impedance Method


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
National Metrology Technical Specifications of the People's Republic of China Impedance method fully automatic microbial detection system Calibration specifications Published on 2023-06-30 Implemented on 2023-12-30 Released by the State Administration for Market Regulation Impedance method fully automatic microbial detection system Calibration specifications Focal point. National Biometrics Technical Committee Main drafting unit. China Institute of Metrology Participating units in the drafting. Hunan Provincial Institute of Metrology and Inspection Chongqing Institute of Metrology and Inspection The National Biometrics Technical Committee is entrusted with the interpretation of this specification. The main drafters of this specification. Zhang Ling (China Institute of Metrology) Sui Zhiwei (China Institute of Metrology) Liu Siyuan (China Institute of Metrology) Participating drafters. Hu Chuzheng (Hunan Provincial Institute of Metrology and Inspection) Peng Jianchun (Chongqing Institute of Metrology and Inspection)

Table of contents

Introduction(Ⅱ) 1 Scope(1) 2 Cited documents(1) 3 Overview(1) 4 Metering characteristics(1) 5 Calibration conditions(2) 5.1 Environmental conditions(2) 5.2 Reference materials and calibration equipment(2) 6 Calibration items and calibration methods (2) 6.1 Temperature indication error, temperature fluctuation and temperature uniformity (2) 6.2 Linear correlation coefficient (3) 6.3 Detection limit(3) 6.4 Repeatability(3) 7 Calibration result expression (4) 8 Recalibration time interval (4) Appendix A Culture media and reagents (5) Appendix B Calibration Certificate Inner Page Format (7) Appendix C Calibration original record format (8) Appendix D Example of uncertainty assessment of temperature indication error calibration results (10)

Introduction

JJF 1071 "Rules for Writing National Measurement Calibration Specifications", JJF 1001 "General Measurement Terms and Definitions" and JJF 1059.1 "Evaluation and Expression of Measurement Uncertainty" together constitute the basic series of regulations supporting the formulation of this specification. Fan. Calibration methods and measurement characteristics mainly refer to JJF 1101-2019 "Environmental Test Equipment Temperature and Humidity Parameters" Calibration Specification", GB 4789.38-2012 "National Food Safety Standard Food Microbiology Testing for Escherichia coli Bacteria Count", GB/T 26428-2010 "Detection of Bacillus subtilis in Feed Microbial Preparations", Foreign Food Inspection Test standard DIN10122-2018 "Determination of microbial enumeration of aerobic and mesophilic bacterial colonies by impedance method for food analysis" "Standard" (and NFV08-105-2010 "Impedance meter method for microbiological examination of food and animal feed guide"). This specification is released for the first time. Impedance method fully automatic microbial detection system Calibration specifications

1 Scope

This specification applies to fully automatic microbial detection systems based on the detection principle of electrical impedance or conductivity measurement. calibration.

2 cited documents

This specification references the following documents. JJF 1101-2019 Specification for calibration of temperature and humidity parameters of environmental test equipment For dated references, only the dated version applies to this specification; for undated references, only the dated edition applies. The latest version (including all modification orders) shall apply to this specification.

3 Overview

The impedance method fully automatic microbial detection system (hereinafter referred to as the detection system) is caused by measuring microbial metabolism An instrument used to measure the microbial content in a sample based on changes in the electrical properties of the culture medium. During the cultivation process, the metabolism of microorganisms Metabolism can metabolize electrically inert macromolecular substrates in the culture medium, such as carbohydrates, proteins, fats and other nutrients. Xie is an electrically active small molecule product that enhances the conductivity of the culture medium. The detection system consists of a temperature control system and an electronic analyzer group become. The growth medium inoculated with the sample is placed in a sample tube equipped with a pair of stainless steel electrodes. The sample tube is assembled The temperature control system and electronic analyzer are cultured in a constant-temperature culture chamber. The detection system records the impedance of each sample every certain time. The change is compared with the internally set positive detection threshold, and the time when the test is positive is recorded. Microbiology according to standard samples Draw the built-in standard curve of the detection system based on the substance concentration and detection time, and compare the calibration curve to calculate the microbial content of the measured sample. content.

4 Metering characteristics

The calibration items and technical indicators of the detection system are shown in Table 1. Table 1 Detection system calibration items and indicators Calibration project measurement characteristics indicators Temperature indication error ±1℃ Temperature fluctuation ±1℃ Temperature uniformity≤2.0℃ Detection limit≤10CFU/mL Linear correlation coefficient R2≥0.98 Repeatability ≤10% Note. The above technical indicators are not used for qualification determination and are for reference only.

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