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Calibration Specification for Adenosine Triphosphate (ATP)Fluorescence Detectors
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JJF 1828-2020
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Basic data Standard ID | JJF 1828-2020 (JJF1828-2020) | Description (Translated English) | Calibration Specification for Adenosine Triphosphate (ATP)Fluorescence Detectors | Sector / Industry | Metrology & Measurement Industry Standard | Classification of Chinese Standard | A50 | Classification of International Standard | 17.020 | Word Count Estimation | 25,240 | Date of Issue | 2020 | Date of Implementation | 2020-04-17 | Issuing agency(ies) | State Administration for Market Regulation |
JJF 1828-2020: Calibration Specification for Adenosine Triphosphate (ATP)Fluorescence Detectors---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Calibration Specification for Adenosine Triphosphate (ATP) Fluorescence Detectors
National Measurement Technical Specification of the People's Republic of China
Calibration specification for ATP fluorescence detector
2020-01-17 release
2020-04-17 Implementation
Issued by the State Administration of Market Supervision and Administration
Calibration specification for ATP fluorescence detector
Focal point. National Biometrics Technical Committee
Main drafting unit. China Academy of Metrology
Participated in the drafting unit. Hebei Institute of Metrology
This specification entrusts the National Biometrics Technical Committee to interpret
The main drafters of this specification.
Fu Boqiang (Chinese Academy of Metrology)
Tang Zhiyu (Chinese Academy of Metrology)
Participating drafters.
Song Zengliang (Hebei Institute of Metrology)
Lu Liang (Chinese Academy of Metrology)
table of Contents
Introduction (Ⅱ)
1 Scope (1)
2 References (1)
3 Terms and units of measurement (1)
3.1 Relative light unit (1)
4 Overview (1)
5 Metrological characteristics (1)
5.1 Background noise value (1)
5.2 Limit of detection (1)
5.3 Linear range and correlation coefficient (1)
5.4 Repeatability (1)
5.5 Relative indication error (2)
6 Calibration conditions (2)
6.1 Environmental conditions (2)
6.2 Reference materials, test swabs or reagents, instruments (2)
7 Calibration items and calibration methods (2)
7.1 Background noise value (2)
7.2 Limit of detection (3)
7.3 Linear range and correlation coefficient (3)
7.4 Repeatability (4)
7.5 Linearity error (5)
8 Expression of calibration results (6)
9 Recalibration interval (6)
Appendix A. Preparation of ATP Standard Working Solutions (7)
Appendix B. Linear Error Calibration Uncertainty Evaluation Example of ATP Substance Measurement (8)
Appendix C. Examples of Uncertainty Evaluation of Linear Error Calibration for Luminance Measurement (13)
Appendix D. ATP Calibration Original Record Format (Recommended Form) (16)
Appendix E. Original Recording Format of Calibration Method for Adjustable Low Light Generator (Recommended Form) (18)
Appendix F Calibration Certificate (Inner Page) Format (Recommended Form) (20)
Introduction
This specification is written in accordance with the requirements of JJF 1071-2010 "Rules for Compiling National Measurement and Calibration Specifications".
This specification mainly refers to ASTM D7463-14a "fuel, fuel/water mixture, fuel-related microbiology
Standard Test Method for the Adenosine Triphosphate (ATP) Content "StandardTestMethodforAdenosine
Triphosphate (ATP) ContentofMicroorganismsinFuel, Fuel/WaterMixtures, and
FuelAssociatedWater] formulation.
This specification is the first release.
Calibration specification for ATP fluorescence detector
1 Scope
This specification applies to the calibration of handheld and desktop ATP fluorescence detectors.
2 Reference documents
This specification refers to the following documents.
ASTMD7463-14a fuel, fuel/water mixture, fuel-related microbial adenosine triphosphate in water
(ATP) content standard detection method [StandardTestMethodforAdenosineTriphosphate (ATP)
ContentofMicroorganismsinFuel, Fuel/WaterMixtures, andFuelAssociatedWater]
For dated reference documents, only the dated version applies to this specification; for those without date references
The latest version (including all amendments) is applicable to this specification.
3 Terms and units of measurement
3.1 relative light unit; RLU [ASTMD7463-14a, 3.1.19]
Adenosine Triphosphate (ATP) is hydrolyzed to Adenosine
Monophosphate (AMP) and pyrophosphate release chemical energy to drive the oxidative release of luciferin catalyzed by luciferase
The unit of measurement of the light quantum number that is put.
Note. Not a SI unit, but proportional to the ATP concentration. Different detectors may produce different values for the same photon number
The same RLU reading.
4 Overview
The ATP fluorescence detector (hereinafter referred to as the instrument) consists of a detection chamber, a control panel, and a photoelectric detector (avalanche diode)
Tube or photomultiplier tube detector, etc.) and data acquisition signal processing system, etc., and equipped with supporting fluorescent
Swab or reagent of a mixture of luciferin and luciferase. After collecting ATP in the sample, mix it with luciferin and luciferase
The biological reaction will produce bioluminescence around 550nm. Within a suitably low ATP concentration range, the luminous intensity is
The ATP concentration is proportional. According to the relative light unit RLU, the sample can be deduced from food residues and microorganisms
The degree of pollution is monitored for overall cleanliness.
The instrument can be divided into handheld and desktop.
5 Metrological characteristics
5.1 Background noise value
5.2 Limit of detection
5.3 Linear range and correlation coefficient
5.4 Repeatability
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