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JJF 1817-2020 English PDF

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JJF 1817-2020: Calibration Specification for Nucleic Acids Analyzers
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Standard ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status	PDF
JJF 1817-2020	English	349	Add to Cart	3 days [Need to translate]	Calibration Specification for Nucleic Acids Analyzers	Valid	JJF 1817-2020

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Basic data

Standard ID	JJF 1817-2020 (JJF1817-2020)
Description (Translated English)	Calibration Specification for Nucleic Acids Analyzers
Sector / Industry	Metrology & Measurement Industry Standard
Classification of Chinese Standard	A50
Classification of International Standard	17.020
Word Count Estimation	15,194
Date of Issue	2020
Date of Implementation	2020-04-17
Issuing agency(ies)	State Administration for Market Regulation

JJF 1817-2020: Calibration Specification for Nucleic Acids Analyzers

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Calibration Specification for Nucleic Acids Analyzers National Measurement Technical Specification of the People's Republic of China Calibration Specification for Nucleic Acid Analyzer 2020-01-17 release 2020-04-17 Implementation Issued by the State Administration of Market Supervision and Administration Calibration Specification for Nucleic Acid Analyzer Focal point. National Biometrics Technical Committee Main drafting unit. China Academy of Metrology Kaijie Enterprise Management (Shanghai) Co., Ltd. Participating in the drafting unit. Chinese Academy of Agricultural Sciences This specification entrusts the National Biometrics Technical Committee to interpret The main drafters of this specification. Dong Lianhua (Chinese Academy of Metrology) Gao Yunhua (Chinese Academy of Metrology) Cao Yan (Kajie Enterprise Management (Shanghai) Co., Ltd.) Participating drafters. Sui Zhiwei (Chinese Academy of Metrology) Li Liang (Chinese Academy of Agricultural Sciences) Wang Jing (Chinese Academy of Metrology)

Introduction (Ⅱ) 1 Scope (1) 2 References (1) 3 Terms and units of measurement (1) 4 Overview (1) 5 Metrological characteristics (1) 5.1 Indication error (1) 5.2 Repeatability (1) 5.3 Fragment concentration linearity (2) 6 Calibration conditions (2) 6.1 Environmental conditions (2) 6.2 Calibration reference materials and calibration equipment (2) 7 Calibration items and calibration methods (2) 7.1 Indication error (2) 7.2 Repeatability (3) 7.3 Concentration linearity (3) 8 Expression of calibration results (4) 9 Recalibration time interval (4) Appendix A. Preparation and use of calibration reagents and solutions (5) Appendix B. Reference Format for Original Calibration Records (6) Appendix C. Reference Format of Internal Page of Calibration Certificate (7) Appendix D Example of uncertainty assessment of concentration indication error (8)

Introduction

The preparation of this specification is based on the test of the main technical parameters of the nucleic acid analyzer, based on JJF 1071-2010 The provisions of "National Rules for the Preparation of Metrology Calibration", JJF 1059.1-2012 "Measurement Uncertainty Evaluation and Expression" Make it. This specification is the first release. Calibration Specification for Nucleic Acid Analyzer

1 Scope

This specification is applicable to nucleic acid analyzers that analyze the length and concentration of nucleic acid fragments based on the principle of gel electrophoresis Calibration, the length range of applicable nucleic acid fragments is (100 ~ 1000) bp, for nuclear The calibration of the acid analyzer can be performed with reference to this specification. This specification does not apply to plate electrophoresis and genetic analyzers calibration.

2 Reference documents

This specification refers to the following documents. JJF 1265-2010 Biometric Terms and Definitions For dated reference documents, only the dated version applies to this specification; for those without date references The latest version (including all amendments) is applicable to this specification.

3 Terms and units of measurement

3.1 Fragment length Represents the size of a nucleic acid fragment in units of nucleotides (nt) or base pairs (bp). 3.2 basepair (bp) basepair Paired bases between two strands in nucleic acids. Such as adenine-thymine (AT) pair, adenine-uracil (A- U), guanine-cytosine (GC) pair, guanine-uracil (GU) pair, etc. The number of base pairs characterizes DNA Or the length of double-stranded RNA. [JJF 1265-2010,4.41]

4 Overview

The nucleic acid analyzer (hereinafter referred to as the analyzer) is based on the principle of gel electrophoresis technology and converts double-stranded nucleic acids of different sizes. The fragments are separated.In the presence of fluorescent dyes, the fluorescent dyes are embedded in the double-stranded nucleic acid to emit fluorescence. Signal detection and analysis equipment can be qualitative and quantitative according to the electrophoresis time and fluorescence signal value of each nucleic acid fragment analysis. The analyzer is usually composed of a sampler, capillary/microchip, fluorescence detection optical components, microcircuit control components, Computer and application software, can be used for single and multiple PCR fragments, restriction enzyme digested DNA, etc. Analysis of fragment length and concentration.

5 Metrological characteristics

5.1 Indication error Fragment length indication error and fragment concentration indication error. 5.2 Repeatability

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