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JJF 1654-2017 English PDF

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JJF 1654-2017: Calibration Specification for Plate Electrophoresis Apparatus
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Basic data

Standard ID JJF 1654-2017 (JJF1654-2017)
Description (Translated English) Calibration Specification for Plate Electrophoresis Apparatus
Sector / Industry Metrology & Measurement Industry Standard
Classification of Chinese Standard A50
Word Count Estimation 14,157
Date of Issue 2017-11-20
Date of Implementation 2018-02-20
Regulation (derived from) AQSIQ Notice 2017 No. 103
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine

JJF 1654-2017: Calibration Specification for Plate Electrophoresis Apparatus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Calibration Specification for Plate Electrophoresis Apparatus People's Republic of China National Metrology Technical Specifications Calibration Specification for Plate Electrophoresis Apparatus Published on.2017-11-20 2018-02-20 Implementation The State Administration of Quality Supervision, Inspection and Quarantine issued Calibration Specification for Plate Electrophoresis Apparatus Undertaking unit. National Biometrics Technical Committee Drafted by. China Academy of Metrology Nanjing Metrological Supervision and Testing Institute Participated in drafting. Henan Academy of Metrology Guangzhou Institute of Metrology and Measurement Technology This specification entrusts the National Biometrics Technical Committee to explain The main drafters of this specification. Sheng Linghui (China Institute of Metrology) Chen Hongfei (Nanjing Metrological Supervision and Inspection Institute) Participating drafters. Wang Jing (China Institute of Metrology) Gao Yunhua (China Institute of Metrology) Ding Fengyuan (Henan Provincial Institute of Metrology) Ling Lingling (Guangzhou Institute of Metrology and Measurement Technology)

table of Contents

Introduction (II) 1 Scope (1) 2 References (1) 3 Terms and units of measure (1) 3.1 Drift (1) 3.2 Electrophoresis bands (1) 3.3 Migration distance (1) 4 Overview (1) 5 Metrological characteristics (1) 5.1 Error indication (1) 5.2 Drift (1) 5.3 Migration distance repeatability (1) 6 Calibration Conditions (1) 6.1 Environmental Conditions (1) 6.2 Calibration Equipment and Reference Materials (2) 7 Calibration Items and Calibration Methods (2) 7.1 Indication Error (2) 7.2 Drift (3) 7.3 Repeatability of Migration Distance (3) 8 Expression of Calibration Results (4) 9 Re-school interval (4) Appendix A Preparation of Calibration Solution for Water Plate Electrophoresis Apparatus (5) Appendix B Preparation of Calibration Solution for Vertical Plate Electrophoresis (6) Appendix C Evaluation Example of Uncertainty for Calibration of Voltage Indication Error (7) Appendix D Calibration Original Record Recommended Format (8) Appendix E Recommended format of calibration page (9)

Introduction

This specification mainly refers to the GB/T 29248-2012 "Basic electrophoresis device", JJG124-2005 ammeter, Voltmeter, power meter, and resistance meter." This specification is the first release. Calibration Specification for Plate Electrophoresis Apparatus

1 Scope

This specification applies to the calibration of the measurement performance of the plate electrophoresis apparatus (horizontal plate electrophoresis apparatus and vertical plate electrophoresis apparatus). Other types of electrophoresis apparatus can be performed with reference to this specification.

2 references

This specification refers to the following documents. JJG124-2005 Ammeter, Voltmeter, Power Meter and Resistance Meter JJF 1071-2010 Rules for the preparation of national metrological calibration specifications GB/T 29248-2012 Basic electrophoresis device For dated references, only dated editions apply to this specification; all undated references The latest version (including all amendments) applies to this specification.

3 Terms and units of measure

3.1 Drift drift The amount of change in the output voltage or current of the electrophoresis device per unit time under specified operating conditions. 3.2 Electrophoresis bands electrophoresisspectrum The band formed by the sample on the carrier after electrophoresis. 3.3 Migration distance migrationlength The distance between the center of the electrophoresis band and the center of the sample hole in electrophoresis is specified in millimeters.

4 Overview

Electrophoresis refers to the phenomenon in which a charged particle moves toward an electrode under the action of an external electric field in a dispersed system. Electrophoresis devices use charged particles such as nucleic acids, proteins and other substances with different charging properties and move under the same electric field conditions. The principle of different directions and mobility is to implement the instrument for the separation and identification of charged particles. The electrophoresis apparatus is usually divided into There are two types of horizontal plates and vertical plates, which are generally made up of main parts such as power supply and electrophoresis tank, as well as auxiliary parts such as glue and cooling parts. composition.

5 Metrological characteristics

5.1 Indication error 5.2 Drift 5.3 Migration distance repeatability

6 Calibration conditions

6.1 Environmental conditions

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