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Calibration Specification for Gel Documentation Systems
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JJF 1530-2015
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Basic data Standard ID | JJF 1530-2015 (JJF1530-2015) | Description (Translated English) | Calibration Specification for Gel Documentation Systems | Sector / Industry | Metrology & Measurement Industry Standard | Classification of Chinese Standard | A61 | Classification of International Standard | 17.020 | Word Count Estimation | 13,177 | Date of Issue | 2015-06-15 | Date of Implementation | 2015-09-15 | Quoted Standard | GB/T 9403-1988; GB 19258-2003; GB/T 20225-2006; GB/T 20733-2006; JB/T 10362-2010; ISO 12233-2000 | Regulation (derived from) | AQSIQ Announcement 2015 No.72 | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine | Summary | This standard applies to calibrated imaging gel imaging system for nucleic acid gel electrophoresis, protein gel electrophoresis, other types of gel imaging system, with reference to these standards. |
JJF 1530-2015: Calibration Specification for Gel Documentation Systems---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Gel imaging system calibration specification)
People's Republic of China National Metrology Technical Specifications
Gel imaging system calibration specifications
Issued on. 2015-06-15
2015-09-15 implementation
The State Administration of Quality Supervision, Inspection and Quarantine released
Gel imaging system calibration specifications
stems
NFP. National Technical Committee biometric
The main drafting units. China Testing Technology Research Institute
Participated in the drafting unit. China Institute of Metrology
This specification entrusted to the National Technical Committee is responsible for interpreting biometric
The main drafters of this specification.
Tan Peace (China Testing Technology Research Institute)
Shen Xing (China Testing Technology Research Institute)
Drafters participate.
Little Jin Qi (China Institute of Metrology)
Su Hongyu (China Testing Technology Research Institute)
Fubo Jiang (China Institute of Metrology)
table of Contents
Introduction (Ⅱ)
1 Scope (1)
2. References (1)
3 terminology and units of measurement (1)
3.1 gray level (1)
UV excitation spectrum 3.2 (1)
4 Overview (1)
4.1 Principle (1)
4.2 Composition (2)
5 Metrological characteristics (2)
5.1 Resolution (2)
5.2 gray level (2)
5.3 Signal to Noise Ratio (2)
5.4 lx (2)
5.5 uneven illumination rate (2)
5.6 UV excitation source spectral radiance relative distribution rate (2)
Calibration Condition 6 (2)
6.1 environmental conditions (2)
6.2 Calibration with a standard device and other equipment (2)
7 calibration items and calibration methods (2)
7.1 Resolution (2)
7.2 gray level (3)
SNR 7.3 (3)
7.4 illumination (3)
7.5 UV excitation source spectral radiance relative distribution rate (4)
8 calibration results expression (4)
9 Recalibration interval (4)
Appendix A calibration certificate within the recommended page format (5)
Appendix B white light illumination calibration results Evaluation of Uncertainty Example (6)
Appendix C UV excitation source calibration device (8)
Introduction
This specification based on the national measurement specifications JJF 1071-2010 "National Calibration Specifications writing rules"
JJF 1001-2011 "common measurement terms and definitions" JJF 1059.1-2012 "Measurement Uncertainty Table
Show "establishment.
Development of this specification primarily with reference to GB/T 9403-1988 "reflective grayscale test chart", GB 19258-
2003 "UV germicidal lamp", GB/T 20225-2006 "Electronic Imaging vocabulary", GB/T 20733-2006 "Digital
Cameras term ", JB/T 10362-2010" digital camera ", ISO 12233-2000" electronic still photography
The camera was measured with a resolution "(Photography-Electronicstil-picturecamerasResolution
measurements) and other standards.
This specification is the first release.
Gel imaging system calibration specifications
1 Scope
This standard applies to imaging calibration gel imaging system for nucleic acid gel electrophoresis, protein gel electrophoresis, which
His type of gel imaging system, can be performed with reference to this specification.
2. References
This specification refers to the following documents.
GB/T 9403-1988 reflective grayscale test chart
GB 19258-2003 UV germicidal lamp
GB/T 20225-2006 Electronic imaging - Vocabulary
GB/T 20733-2006 digital camera terms
JB/T 10362-2010 Digital Camera
ISO 12233-2000 photography electronic still camera resolution measurement (Photography-
Electronicstil-picturecamerasResolutionmeasurements)
For dated references, only the dated edition applies to this specification; undated reference documents
Member, the latest edition (including any amendments) applies to this specification.
3 terminology and units of measurement
GB 19258-2003 UV germicidal lamp, GB/T 20225-2006 Electronic Imaging vocabulary,
GB/T 20733-2006 digital camera terminology, JB/T 10362-2010 and digital cameras, as defined in the following
The terms and definitions apply to this specification.
3.1 gray level grayscale
Gel imaging system for quantitative image lightness or darkness of the description.
3.2 UV excitation spectrum ultravioletexcitationspectrum
After the spectrum called the spectrum is the polychromatic light through the dispersion system (such as a grating, prism) spectral components, in accordance with
Optical wavelength (or frequency) of the size of the sequential arrangement of patterning. Gel imaging system ultraviolet excitation source of UV excitation
It is specific wavelength spectrum 200nm ~ 400nm near-ultraviolet spectrum.
4 Overview
Gel imaging system is a black box to be placed within the nucleic acid gel electrophoresis, protein gel electrophoresis using an imaging device into
Line imaging, image acquisition and entered into the computer, through appropriate analysis software, the gel strip for the qualitative and semiquantitative
Analysis of the amount of the instrument.
4.1 Principle
Since the sample on an electrophoretic gel mobility is not the same, or other alternative to the standard reference for reference, the root
According to an unknown location in the sample spectrum can be estimated in the relative molecular mass size, which is qualitative analysis. Concentrated in certain
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