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GHT1312-2020: The determination of chlorogenic acid, caffeic acid, p-coumaric acid, 3, 5-dicaffeoylquinic acid, 4, 5-dicaffeoylquinic acid and artepillin C in propolis - High performance liquid chromatography
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GH/T 1312-2020 | English | 229 |
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The determination of chlorogenic acid, caffeic acid, p-coumaric acid, 3, 5-dicaffeoylquinic acid, 4, 5-dicaffeoylquinic acid and artepillin C in propolis - High performance liquid chromatography
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GH/T 1312-2020
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PDF similar to GHT1312-2020
Basic data | Standard ID | GH/T 1312-2020 (GH/T1312-2020) | | Description (Translated English) | The determination of chlorogenic acid, caffeic acid, p-coumaric acid, 3, 5-dicaffeoylquinic acid, 4, 5-dicaffeoylquinic acid and artepillin C in propolis - High performance liquid chromatography | | Sector / Industry | Supply and Marketing Cooperatives Industry Standard (Recommended) | | Classification of Chinese Standard | B47 | | Classification of International Standard | 65.140 | | Word Count Estimation | 10,122 | | Date of Issue | 2020-12-07 | | Date of Implementation | 2021-03-01 | | Regulation (derived from) | China National Supply and Marketing Cooperatives Announcement No. 2 of 2020 | | Issuing agency(ies) | All-China Federation of Supply and Marketing Cooperatives | GHT1312-2020: The determination of chlorogenic acid, caffeic acid, p-coumaric acid, 3, 5-dicaffeoylquinic acid, 4, 5-dicaffeoylquinic acid and artepillin C in propolis - High performance liquid chromatography
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
The determination of chlorogenic acid, caffeic acid, p-coumaric acid, 3, 5-dicaffeoylquinic acid, 4, 5-dicaffeoylquinic acid and artepillin C in propolis--High performance liquid chromatography
ICS 65.140
CCSB47
Industry Standards for Supply and Marketing Cooperation of the People's Republic of China
Chlorogenic acid, caffeic acid, p-coumaric acid,
3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid
And atipillin C. Determination of high performance liquid chromatography
2020-12-07 released
2021-03-01 implementation
Released by All China Supply and Marketing Cooperatives
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
This document was proposed by the All-China Federation of Supply and Marketing Cooperatives.
This document is under the jurisdiction of the National Bee Product Standardization Working Group (SAC/SWG2).
Drafting organizations of this document. Zhejiang University, Hangzhou Fengqing Technology Co., Ltd., Feng Naibao Honpo (Nanjing) Health Food Co., Ltd., Jiangshan Fu
Cited Bee Industry Technology Development Co., Ltd., Xiamen Sijian Biological Technology Co., Ltd., Changxing Yifeng Bee Industry Technology Co., Ltd.
The main drafters of this document. Hu Fuliang, Zhang Cuiping, Liu Gang, Liu Jia, Xu Shuirong, Yan Jianxiang, Qiu Rumin.
Introduction
The issuing agency of this document draws attention to the fact that when the declaration conforms to this document, the patent (ZL201610279885.2) related to this document may be involved.
use.
The issuing agency of this document has no position on the authenticity, validity and scope of the patent.
The patent holder has assured the issuing organization of this document that he is willing to work with any applicant under reasonable and non-discriminatory terms and conditions.
Negotiations on the licensing of patents. The statement of the patent holder has been filed with the issuing agency of this document. Relevant information can be through the following
Contact information.
Patent holder. Zhejiang University, Feng Naibao Honpo (Nanjing) Health Food Co., Ltd.
Address. No. 866, Yuhangtang Road, Xihu District, Hangzhou City, Zhejiang Province.
Please note that in addition to the above-mentioned patents, certain contents of this document may still involve patents. The issuing agency of this document is not
Li’s responsibility.
Chlorogenic acid, caffeic acid, p-coumaric acid,
3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid
And atipillin C. Determination of high performance liquid chromatography
1 Scope
This document specifies the high performance liquid chromatography method for the determination of chlorogenic acid, caffeic acid, p-coumaric acid, 3,5-dicaffeoylquinic acid, 4,5-bis
Caffeoylquinic acid and atipillin C method.
This document is applicable to chlorogenic acid, caffeic acid, p-coumaric acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid and ati in propolis.
Quantitative determination of Pilin C.
2 Normative references
The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations
Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
This document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Propolis
Worker bees collect the gluey substance formed by mixing plant secretions such as resin with secretions from the mandibular glands and wax glands.
4 Principle
The sample was ultrasonically extracted with 85% ethanol, centrifuged, and filtered, and chlorogenic acid, chlorogenic acid,
Caffeic acid, p-coumaric acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid and atipillin C were quantified by external standard method.
5 Reagents and materials
Unless otherwise specified, all reagents are analytically pure, and the experimental water should meet the requirements of the first grade water in GB/T 6682.
5.1 Methanol. chromatographically pure.
5.2 Absolute ethanol.
5.3 Acetic acid. chromatographically pure.
5.4 0.1% acetic acid solution. Measure 1mL of acetic acid (5.3) into 1000mL of water and mix well, and set aside.
5.5 Chlorogenic acid. CAS number 327-97-9, purity ≥95%; Caffeic acid. CAS number 331-39-5, purity ≥98%; p-coumaric acid. CAS
No. 501-98-4, purity ≥98%; 3,5-dicaffeoylquinic acid. CAS number 2450-53-5, purity ≥98%; 4,5-dicaffeoylquinine
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