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(Zanthoxylum bungeanum and its processed products-Determination of total prickly amide content-Ultraviolet spectrophotometry)
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Basic data | Standard ID | GH/T 1290-2020 (GH/T1290-2020) | | Description (Translated English) | (Zanthoxylum bungeanum and its processed products-Determination of total prickly amide content-Ultraviolet spectrophotometry) | | Sector / Industry | Supply and Marketing Cooperatives Industry Standard (Recommended) | | Classification of Chinese Standard | B36 | | Classification of International Standard | 67.220.10 | | Date of Issue | 2020-06-04 | | Date of Implementation | 2020-09-01 | | Regulation (derived from) | Industry Standard Information Service Platform (2020.07.22) | | Issuing agency(ies) | All-China Federation of Supply and Marketing Cooperatives | GHT1290-2020: (Zanthoxylum bungeanum and its processed products-Determination of total prickly amide content-Ultraviolet spectrophotometry)
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Prickly ash and its processed products-Determination of total fagaramide- Ultraviolet spetrophotometry
ICS 67.220.10
B 36
GH
Industry Standards for Supply and Marketing Cooperation of the People's Republic of China
Determination of the total content of prickly amide in Zanthoxylum bungeanum and its processed products
UV Spectrophotometry
2020-06-04 release
2020-09-01 Implementation
Released by China National Supply and Marketing Cooperatives
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Appendix A of this standard is an informative appendix.
This standard was proposed by the All-China Federation of Supply and Marketing Cooperatives.
This standard is under the jurisdiction of the National Spice Standardization Technical Committee (SAC/TC 408).
Drafting organizations of this standard. All China Supply and Marketing Cooperatives Nanjing Institute of Comprehensive Utilization of Wild Plants, Shaanxi Weikang Food Technology Co., Ltd.
Co., Ltd., Chenguang Biological Technology Group Co., Ltd., Zhumadian Wangshouyi Shisanxiang Condiment Group Co., Ltd., Shandong Baijia Food
Co., Ltd., Qingdao Lihe Extraction Co., Ltd., Seasoning Branch of China Chamber of Commerce for Food, Native Produce and Animal Import and Export.
The main drafters of this standard. Liu Yingjie, Liu Qiang, Liu Dengshuai, Zhang Hui, Zhao Botao, Liu Jihua, Jiao Liwei, Wu Yaojun, Huo Shuxiang,
Wang Yinliang, Wang Yangai, Huang Xiaode, Su Hai, Li Yi, Zhang Yongchang, Sun Xili.
Zanthoxylum bungeanum and Zanthoxylum bungeanum processed products. Determination of total content of prickly amide. Ultraviolet spectrophotometric method
1 Scope
This standard specifies the ultraviolet spectrophotometric method for the determination of the total content of prickly amide in Zanthoxylum bungeanum and its processed products.
This standard applies to the analysis and inspection of Zanthoxylum bungeanum and its processed products.
2 Normative references
The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this document.
For undated references, the latest version (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 12729.2 Sampling method for spices and condiments
GB/T 12729.3 Preparation of powder samples for analysis of spices and condiments
3 Terms and definitions
3.1
Prickly ash products
Zanthoxylum bungeanum is a product produced by processes such as crushing and extraction. Mainly including prickly ash powder, prickly ash oleoresin, prickly ash oil and other processing with prickly ash as raw materials
The product.
3.2
Fagaramide
Zanthoxylum bungeanum is the main ingredient of numb flavor, containing hydroxy-α-sanshool, hydroxy-β-sanshool, hydroxy-γ-sanshool and hydroxy-ε-sanshool, etc.
Kind of amide ingredients.
4 Principle
Xanthoxyamide mainly contains amide components such as hydroxy-α-sanshool, hydroxy-β-sanshool, hydroxy-γ-sanshool and hydroxy-ε-sanshool.
The prickly amide component in the sample is extracted with methanol, and has a maximum absorption at 270 nm wavelength. The absorbance is related to the content of prickly amide in the extract
There is a positive correlation. The absorption coefficients of the extracts of different types of samples are different at 270 nm. The absorption coefficient of the sample
The total amount of prickly amide is determined.
5 Reagent materials and equipment
5.1 Reagent materials
5.1.1 Methanol. chromatographically pure.
5.1.2 Microporous filter membrane. organic phase filter membrane with a pore size of 0.22 μm.
5.2 Equipment
5.2.1 Ultraviolet-visible spectrophotometer with 1 cm quartz cuvette.
5.2.2 Analytical balance, with a sensitivity of 0.0001 g.
5.2.3 Universal grinder, rotating speed ≥10000 rpm.
5.2.4 Tissue crusher, rotating speed ≥300 rpm.
5.2.5 Constant temperature water bath.
5.2.6 Ultrasonic cleaner, ultrasonic power 240 W.
6 Sample processing
6.1 Dried pepper
Take 100 g of the well-mixed peppercorns, pulverize with a universal grinder, pass through a 20-mesh sieve, and mix. Weigh 1g (accurate to 0.0001 g) and crush
Place the mixed sample in a flask, add 50 mL of methanol, and ultrasonically extract for 0.5 h, cool to room temperature, and transfer to a 100 mL volumetric flask.
Constant volume, transfer 10 mL of the supernatant to a 50 mL volumetric flask to constant volume, filter through 0.22 μm filter membrane, to be tested.
6.2 Fresh pepper
Take.200 g of freshly mixed fresh pepper seeds. After being crushed by the tissue crusher, weigh 5 g (accurate to 0.0001 g) of the sample after crushing and mixing
Place it in a flask with a stopper, add 50 mL of methanol, extract with ultrasonic for 0.5 h, cool to room temperature, transfer to a 100 mL volumetric flask, dilute the volume of methanol, transfer
Take 10 mL of the supernatant in a 50 mL volumetric flask and filter through a 0.22 μm filter membrane for testing.
6.3 Pepper powder
Weigh 1 g (accurate to 0.0001 g) of the mixed sample and place it in a flask with a stopper, add 50 mL of methanol, extract with ultrasound for 0.5 h, and cool to the chamber
Temperature, transfer to a 100 mL volumetric flask, make constant volume with methanol, transfer 10 mL of the supernatant to a 50 mL volumetric flask, and filter through a 0.22 μm filter membrane.
To be tested.
6.4 Zanthoxylum oleoresin
Take the paste or solid oleoresin sample to dissolve in a 50 ℃ water bath and shake well. Precisely weigh 0.2 g (accurate to 0.0001 g) sample set
In a beaker with a stopper, add 50 mL of methanol, ultrasonically extract for 0.5 h, cool to room temperature, and transfer to a 100 mL volumetric flask.
Filtration with μm filter membrane, to be tested.
6.5 Pepper Oil
Precisely weigh 0.2 g of pepper oil (accurate to 0.0001 g) into a flask with a stopper, add 50 mL of methanol, and ultrasonically extract for 0.5 h.
Cool to room temperature, transfer to a 100 mL volumetric flask, make methanol to a constant volume, filter through a 0.22 μm filter membrane, and wait for measurement.
7 Determination
Place the sample solution in a 1 cm cuvette, use methanol as a reference, and measure the absorbance at 270 nm. The absorbance exceeds the measurement range of the instrument.
The sample solution should be diluted with methanol and measured.
8 Result calculation
The total content of Xanthoxyamide in the sample is calculated as hydroxy-α-sanshool, and the unit is expressed in milligrams per gram (mg/g), calculated according to formula (1).
X--The content of prickly amides in the sample, in milligrams per gram (mg/g);
A--The absorbance of the sample solution at 270 nm;
V-constant volume volume, the unit is milliliters (mL);
K--The dilution factor of the sample solution;
m--the mass of the sample, in grams (g);
E--Absorption coefficient, the absorbance value of 1 mg/mL prickly amide solution at 270nm, where the coefficient of prickly ash and prickly ash powder is 410,
The coefficient of pepper oleoresin and pepper oil products is 380.
Take the arithmetic mean of two independent determination results with absolute difference not greater than 5% of the arithmetic mean under repeatability conditions as the measured value. Calculation
The result is rounded to 2 decimal places.
AA
Appendix A
(Informative appendix)
Typical UV absorption spectrum
A.1 The ultraviolet absorption spectrum of hydroxy-α-sanshool is shown in Figure A.1.
Figure A.1 Typical UV absorption spectrum of hydroxy-α-sanshool
A.2 The typical UV absorption spectrum of the Zanthoxylum bungeanum sample is shown in Figure A.2.
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