|
US$159.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GBZT315-2018: Determination of chromium in blood -- Graphite furnace atomic absorption spectrometric method
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GBZ/T 315-2018 | English | 159 |
Add to Cart
|
3 days [Need to translate]
|
Determination of chromium in blood -- Graphite furnace atomic absorption spectrometric method
| Valid |
GBZ/T 315-2018
|
PDF similar to GBZT315-2018
Standard similar to GBZT315-2018 GBZ 20 GBZ 57 GBZ 49 GBZ/T 325 GBZ 324 Basic data | Standard ID | GBZ/T 315-2018 (GBZ/T315-2018) | | Description (Translated English) | Determination of chromium in blood -- Graphite furnace atomic absorption spectrometric method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C60 | | Word Count Estimation | 8,848 | | Date of Issue | 2018-08-16 | | Date of Implementation | 2019-01-01 | | Older Standard (superseded by this standard) | WS/T 38-1996 | | Regulation (derived from) | State-Health-Communication (2018) No.14 | | Issuing agency(ies) | National Health and Family Planning Commission | GBZ/T 315-2018: Determination of chromium in blood -- Graphite furnace atomic absorption spectrometric method
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of chromium in blood - Graphite furnace atomic absorption spectrometric method
CS 13.100
C 52
National Occupational Health Standards
Replace WS/T 38-1996
Determination of chromium in blood by graphite furnace atomic absorption spectrometry
Determination of chromium in blood-
Graphite furnace atomic absorption spectrometric method
Published on.2018 - 08 - 16
2019 - 01 - 01 implementation
National Health and Wellness Committee of the People's Republic of China
Foreword
This standard is formulated in accordance with the Law of the People's Republic
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard replaces WS/T 38-1996 "Method for the determination of chromium in blood by graphite furnace atomic absorption spectrometry".
Compared with WS/T 38-1996, the main changes are as follows.
-- The operating conditions of the instrument are changed to the reference conditions for instrument operation;
-- Improved standard formulation and sample handling methods;
-- Increased use of matrix modifiers.
This standard was drafted. Jiangsu Provincial Center for Disease Control and Prevention, China Center for Disease Control and Prevention, Occupational Health and Poison Control Institute, Guangdong
Provincial Occupational Disease Prevention and Control Institute, Wuxi Municipal Center for Disease Control and Prevention, Nanjing Occupational Disease Prevention and Control Institute.
The main drafters of this standard. Zhong Lixin, Zhu Baoli, Zhang Hengdong, Jiang Dong, Yan Huifang, Zhang Jing, Zhang Aihua, Dong Ming, Liu Wenwei, Meng
Yuan Hua, Xing Yan, Zhou Tongzhou.
The previous versions of the standards replaced by this standard.
--WS/T 38-1996.
Determination of chromium in blood by graphite furnace atomic absorption spectrometry
1 Scope
This standard specifies graphite furnace atomic absorption spectrometry for the determination of chromium in blood.
This standard applies to the determination of chromium in the blood of occupational contacts.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this document.
For undated references, the latest edition (including all amendments) applies to this document.
GBZ /T 295 General rules for occupational health biological monitoring
WS/T 97 urinary creatinine spectrophotometric method
WS/T 98 urinary creatinine by reversed-phase high performance liquid chromatography
3 Principle
The blood sample was diluted with a matrix modifier and measured by graphite furnace atomic absorption spectrometry at a wavelength of 357.9 nm.
4 instruments
4.1 Volumetric flask, 10 mL, 100 mL, 500 mL, 1000 mL.
4.2 Pipettes, 50 μL,.200 μL, 1000 μL.
4.3 Balance, the sensitivity is 0.1mg.
4.4 Covered plastic centrifuge tube, 1.5 mL.
4.5 Blood collection supplies, blood collection needles, sterile cotton swabs, lithium heparin (sodium) vacuum blood collection tubes, etc.
4.6 Atomic absorption spectrometer with graphite furnace atomizer, Zeeman calibration device, chrome hollow cathode lamp.
5 reagent
5.1 Experimental water, deionized water or quartz glass sub-boiling distilled water.
5.2 Nitric acid, high purity.
5.3 Triton X-100, chromatographically pure.
5.4 Magnesium nitrate hexahydrate, excellent grade.
5.5 Matrix modifier. Weigh 0.865 g of magnesium nitrate hexahydrate dissolved in water, add 1.0 mL Triton X-100, 2.0 mL
Nitric acid, make up to 1000 mL.
5.6 Bovine blood, heparin anticoagulation, stored at -20 ° C, shake to room temperature when used. Low background blood can also be used.
5.7 Chromium standard stock solution. Accurately weigh the reference material potassium dichromate (110 °C, baking for 2 h) 1.4315 g (accurate to 0.0001 g),
After dissolving with a small amount of water, transfer the whole amount to a 500 mL volumetric flask, add 5.0 mL of nitric acid, dilute to volume with water, and mix. This solution per ml
Contains 1.0 mg of chromium. Or purchase a standard stock of chromium monoliths certified by the state and awarded a certificate of reference material.
5.8 Chromium standard intermediate solution [ρ(Cr)=5.0 μg/mL]. The chromium monolith standard stock solution is diluted to a concentration of 5.0 with a matrix modifier.
Standard intermediate solution of μg/mL.
5.9 Chromium standard series solution. respectively absorb chromium standard intermediate solution (5.0 μg/mL) 0.0 μL, 30.0 μL, 50.0 μL, 100.0 μL,
200.0 μL, 400.0 μL, and 800.0 μL in a 10 mL volumetric flask, dilute to the mark with a matrix modifier, and mix. In each volumetric flask
Each liter contains 0.0 μg, 15.0 μg, 25.0 μg, 50.0 μg, 100.0 μg,.200.0 μg, and 400.0 μg of chromium. Temporary
Now equipped.
6 Sample collection, transportation and storage
According to the requirements of GBZ /T 295, the venous blood of workers exposed to chromium should be collected more than 2.0 mL, shake immediately and refrigerate. Sample can be guaranteed at 4 °C
It can be stored for two weeks at -20 °C for two months.
7 Analysis steps
7.1 Instrument Operation Reference Conditions
The atomic absorption spectrometer with Zeeman correction device was commissioned to the optimum condition with reference to the following instrument conditions.
a) wavelength. 357.9 nm;
b) slit. 0.7 nm;
c) Injection volume. 10 μL;
d) Graphite furnace conditions.
-- Drying. room temperature ~ 110 ° C, 20 s; 110 ° C ~ 130 ° C, 30 s;
-- Ashing. 1300 ° C, 20 s;
-- Atomization. 2300 ° C, 5 s (gas cut);
-- Clear. 2500 °C, 5 s.
7.2 Sample Pretreatment
Place the blood sample at room temperature, shake well, and aspirate 0.10 mL of the blood sample into a 1.5 mL plastic centrifuge tube. Add 0.90 mL matrix to improve.
The agent is thoroughly mixed and tested; at the same time, 0.10 mL of water is used instead of the blood sample, and the sample is treated as the reagent blank.
7.3 Preparation and determination of the working curve
Take 7 1.5 mL plastic centrifuge tubes with caps and draw 0.1 mL of 0.0μg/L～400.0 μg/L standard series solution, 0.1 mL of bovine blood, 0.8
The mL matrix modifier is mixed in a centrifuge tube and sampled according to the instrument operating reference conditions. See Table 1 for specific operations. According to the absorbance of each tube
The regression equation was calculated from the difference between the standard series 1 tube absorbance and the chromium concentration (μg/L).
Table 1 blood chromium working curve standard series solution preparation
Test tube number 1 2 3 4 5 6 7
Chromium standard series solution/mL 0.10 0.10 0.10 0.10 0.10 0.10 0.10
Bovine blood/mL 0.10 0.10 0.10 0.10 0.10 0.10 0.10
Matrix improver/mL 0.80 0.80 0.80 0.80 0.80 0.80 0.80
Chromium concentration/(μg/L)
Bovine blood
Bottom value
Bovine blood
Bottom value 15
Bovine blood
Bottom value 25
Bovine blood
Bottom value 50
Bovine blood background
Value 100
Bovine blood background
Value.200
Bovine blood background
Value 400
7.4 Determination
Determination of reagent blanks, sample blanks, and sample solutions using the operating conditions of the standard series of solutions, and blank and absorbance of the measured samples and samples
After subtracting the reagent blank absorbance value from the degree value, the concentration of chromium (μg/L) in the sample was calculated from the regression equation.
8 calculation
The concentration of chromium in the blood sample is calculated according to formula (1).
1cc ...(1)
In the formula.
c -- the concentration of chromium in the blood sample, in micrograms per liter (μg/L);
C1 -- The concentration of chromium in the blood sample calculated by the regression equation in micrograms per liter (μg/L).
9 Description
9.1 The detection limit of the method is 0.47 μg/L, and the lower limit of quantification is 1.56 μg/L; the method range is 1.56 μg/L to 400.0 μg/L.
Within this range, the correlation coefficient is >0.9990; the precision within the method range is 0.6% to 6.6% (the blood chromium concentration is 4.1 μg/L to 306.1).
Gg/L, n=6); the inter-assay precision range is 1.0% to 6.7% (blood chromium concentration is 4.1 μg/L to 302.4 μg/L, n=6);
The recovery of spiked blood samples was 94.5 %~104.1% (the concentration of blood chromium was 3.2 μg/L～101.2 μg/L, and the spiked concentration was 25.0.
Gg/L ~ 300.0 μg/L, n = 6).
9.2 This method is used for blood chromium determination. The minimum sample volume of blood sample is 50.0 μL.
9.3 All the utensils used in the experiment should be soaked in nitric acid solution (1 4) overnight, rinsed repeatedly with water, and finally rinsed with deionized water.
9.4 When this method is used for the determination of blood chromium, it should not be analyzed by graphite furnace atomic absorption spectrometer with xenon lamp calibration device, otherwise it will lead to
The detection limit and the lower limit of quantitation are both high.
9.5 1000.0 μg/mL of Na, K, Ca2, Mg2, Cu2, Zn2, 500.0 μg/mL of Fe3, 100.0 μg/mL of Li,
Si
The Ba2, Be2, Al3, Ni2, Mn2, and Pb2 ions do not interfere with the measurement.
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GBZT315-2018_English be delivered?Answer: Upon your order, we will start to translate GBZT315-2018_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of GBZT315-2018_English with my colleagues?Answer: Yes. The purchased PDF of GBZT315-2018_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.
|