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GBZT314-2018 English PDF

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GBZT314-2018: Determination of nickel in blood -- Graphite furnace atomic absorption spectrometry method
Status: Valid

Standard ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status	PDF
GBZ/T 314-2018	English	139	Add to Cart	3 days [Need to translate]	Determination of nickel in blood -- Graphite furnace atomic absorption spectrometry method	Valid	GBZ/T 314-2018

PDF similar to GBZT314-2018

Standard similar to GBZT314-2018

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Basic data

Standard ID	GBZ/T 314-2018 (GBZ/T314-2018)
Description (Translated English)	Determination of nickel in blood -- Graphite furnace atomic absorption spectrometry method
Sector / Industry	National Standard (Recommended)
Classification of Chinese Standard	C60
Word Count Estimation	7,798
Date of Issue	2018-08-16
Date of Implementation	2019-01-01
Older Standard (superseded by this standard)	WS/T 45-1996
Regulation (derived from)	State-Health-Communication (2018) No.14
Issuing agency(ies)	National Health and Family Planning Commission

GBZ/T 314-2018: Determination of nickel in blood -- Graphite furnace atomic absorption spectrometry method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of nickel in blood - Graphite furnace atomic absorption spectrometry method ICS 13.100 C 52 National Occupational Health Standards Replace WS/T 45-1996 Determination of nickel in blood by graphite furnace atomic absorption spectrometry Determination of nickel in blood- Graphite furnace atomic absorption spectrometry method Published on.2018 - 08 - 16 2019 - 01 -01 Implementation National Health and Wellness Committee of the People's Republic of China

Foreword

This standard is formulated in accordance with the Law of the People's Republic This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard replaces WS/T 45-1996 "Method for the determination of nickel in blood by graphite furnace atomic absorption spectrometry". The main technical changes compared to WS/T 45-1996 are as follows. -- Improved sample handling method with 0.1% Triton X-100 solution as diluent. This standard was drafted. Shenzhen Occupational Disease Prevention and Treatment Institute, Shenzhen Baoan District Center for Disease Control and Prevention, Yantian District, Shenzhen, disease prevention Control Center, Shenzhen Luohu District Center for Disease Control and Prevention. The main drafters of this standard. Cai Jinmin, He Juntao, Yan Jianpei, Yin Jiangwei, Lu Haiyan, Ye Min. Determination of nickel in blood by graphite furnace atomic absorption spectrometry

1 Scope

This standard specifies graphite furnace atomic absorption spectrometry for the determination of nickel in blood. This standard applies to the determination of nickel in the blood of occupational contacts.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this document. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods General rules for biological monitoring of GBZ /T 295 occupational population

3 Principle

Blood samples were diluted with 0.1% Triton X-100 solution and measured by graphite furnace atomic absorption spectrometry at 232.0 nm.

4 instruments

4.1 Atomic absorption spectrophotometer with graphite furnace, Zeeman or other background correction device and nickel hollow cathode lamp. 4.2 Volumetric flask, 50 mL, 100 mL. 4.3 Analyze the balance with a sensation of 0.1 mg. 4.4 Pipettes, 50 L, 100 L,.200 L, 1000 L. 4.5 covered plastic centrifuge tube, 2 mL. 4.6 vortex oscillator.

5 reagent

5.1 Experimental water. Performed in accordance with GB/T 6682 standard. 5.2 Nitric acid. ρ20=1.42 g/mL, excellent grade pure. 5.3 Heparin sodium blood collection tube. excellent grade pure. 5.4 Nitric acid solution. 5 mL of nitric acid was added to 95 mL of water. 5.5 Heparin sodium solution. Weigh 0.5 g of sodium heparin, dissolve in water, add water to 100 mL. 5.6 Triton X-100. Analytical grade. 5.7 Diluent. Take 0.1 mL of Triton X-100, dissolve in an appropriate amount of water, and dilute to 100 mL. 5.8 Nickel Standard Solution. Prepared with a nationally recognized nickel standard solution. 6 Sample collection, transportation and storage 2 mL of blood samples exposed to nickel workers were collected with heparin sodium anticoagulated blood vessels and transported at room temperature or refrigerated. The samples were stored at 4 ° C and stored for up to 7 days. If it is not refrigerated, store it at room temperature for up to 3 days.

7 Analysis steps

7.1 Instrument Operation Reference Conditions Refer to Table 1 for instrument operation reference conditions. Table 1 Instrument operation reference conditions Instrument condition Graphite furnace condition Step temperature Heating time Residence time Wavelength 232.0 nm 1. Drying room temperature ~ 110 5 30 Slit 0.2 nm -- 110 to 130 10 30 Lamp current 25 mA 2. Ashing 130~450 10 10 Injection volume 20 L -- 450～1500 10 20 Carrier gas (Argon) 250 mL/min 3. Atomization 2300 0 5 (discontinued) 4. Cleaning 2500 0 5 Background correction Zeeman or other --- -- -- 7.2 Preparation and determination of the working curve The nickel standard solution was diluted with a dilution solution into 0.5 g/mL nickel standard application solution, and 7 plastic centrifuge tubes with lids were prepared and prepared according to Table 2. A standard series of solutions with a concentration of 0 g/L to 160 g/L, in which each tube is mixed with 100 L normal human mixed blood, supplemented with diluent to the whole Accumulate 1.00 mL, mix and measure with reference to the operating conditions of the instrument. See Table 1 for specific operations. Subtract the standard series 2 to 7 tube absorbance values to the standard series After 1 tube absorbance value, the regression equation was calculated with nickel concentration (g/L). The preparation of the working curve is shown in Table 2. Table 2 Preparation of working curve Pipe number 1 2 3 4 5 6 7 0.5 g/mL nickel standard Application fluid, mL 0 0.01 0.02 0.04 0.08 0.16 0.32 Normal human blood, mL 0.10 0.10 0.10 0.10 0.10 0.10 0.10 Diluent, mL 0.90 0.89 0.88 0.86 0.82 0.74 0.58 Nickel content, g/L blood background value Blood background Blood background Blood background Blood background Blood background Blood background 7.3 Sample processing and determination 7.3.1 Sample Processing The blood sample was taken out of the refrigerator and returned to room temperature. After thoroughly shaking the blood sample, dilute it 10 times with the diluent and mix for measurement. 7.3.2 Sample blank Take heparin sodium blood collection tube, add 1 mL water, dissolve heparin sodium, mix well, take 100 L of this heparin sodium solution, dilute 10 times with dilution solution, Mix well for measurement. 7.3.3 Sample determination Determine the treated sample and sample blanks by measuring the operating conditions of the calibration series solution, and obtain blood samples and samples from the working curve or regression equation. The corresponding concentration values of nickel in the blanks are subtracted from each other to obtain the concentration of nickel in the blood sample after treatment. Or subtract the sample blank from the absorbance of the sample. After the absorbance, divided by the slope of the regression equation, the concentration of nickel in the blood sample after treatment is obtained. Before and after the measurement and after every 10 to 30 samples are measured, The quality control sample was measured once.

8 calculation

Calculate the concentration of nickel in the blood according to formula (1). X = 10c ... (1) In the formula. X - the concentration of nickel in the blood, in micrograms per liter (g/L); 10 -- blood sample dilution factor; c -- The concentration of nickel in the blood sample after treatment (minus the sample blank) in micrograms per liter (g/L).

9 Description

9.1 The detection limit of this method is 1.13 g/L; the minimum detection concentration is 11.3 g/L (10 times dilution); the lower limit of quantification is 3.76 g/L; The minimum quantitative concentration is 37.6 g/L (10 times dilution); the linear range of the working curve is 1.13 g/L～160 g/L; The quasi-deviation. RSD=1.2%~4.3%; the recovery rate of blood samples was 98.5 %~108.0%. 9.2 Equipment cleaning. Glass and plastic utensils are soaked in 10% nitric acid solution for about 12 h, rinse off. Dust away after drying. 9.3 Sampling requirements. For workers exposed to soluble nickel salts, post-class blood should be collected, which represents the contact situation for one working day. Before sampling, Workers should take off the contact site, take off their overalls, wash hands, face and sampling area, and then sample in a clean, non-polluting place. In turn Thoroughly scrub the sampling site with 0.5% nitric acid and deionized water, then sterilize and take blood to prevent external contamination. After blood collection, shake gently to make blood Mix the liquid thoroughly with the anticoagulant, but avoid strong shaking. Sample collectors must be proficient in the knowledge of sample collection, transport and preservation. technology. 9.4 When the blood nickel concentration is 457 g/L, 1000 g/mL of sodium, potassium, calcium, magnesium, copper, manganese, chromium, lead, zinc, arsenic, cadmium, zirconium, 500 g/mL of iron, cobalt, 100 g/mL of lithium, lanthanum, cerium, lanthanum, cerium, lanthanum, vanadium, molybdenum and tungsten did not interfere with the measurement. 9.5 If the blood nickel concentration is outside the measurement range, the dilution factor may be increased, but the normal human blood in the working curve should also be the same dilution factor. 9.6 The quality assurance of the testing process shall be carried out in accordance with the requirements of GBZ /T 295.

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