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GBZ42840-2023: Nanotechnologies - Determination of silver nanoparticles potency by release of muramic acid from Staphylococcus aureus
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| GB/Z 42840-2023 | English | 334 |
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Nanotechnologies - Determination of silver nanoparticles potency by release of muramic acid from Staphylococcus aureus
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GB/Z 42840-2023
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Basic data | Standard ID | GB/Z 42840-2023 (GB/Z42840-2023) | | Description (Translated English) | Nanotechnologies - Determination of silver nanoparticles potency by release of muramic acid from Staphylococcus aureus | | Sector / Industry | National Standard | | Classification of Chinese Standard | A43 | | Classification of International Standard | 07.030 | | Word Count Estimation | 18,139 | | Date of Issue | 2023-08-06 | | Date of Implementation | 2023-08-06 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GBZ42840-2023: Nanotechnologies - Determination of silver nanoparticles potency by release of muramic acid from Staphylococcus aureus
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GB /Z 42840-2023.Nanotechnology uses muramic acid released by Staphylococcus aureus to detect the efficacy of silver nanoparticles
ICS 07.030
CCSA43
National Standardization Guiding Technical Documents of the People's Republic of China
Nanotechnology harnesses Staphylococcus aureus to release
Muramic acid detects the efficacy of silver nanoparticles
(ISO /T S16550.2014,IDT)
Published on 2023-08-06
2023-08-06 Implementation
State Administration for Market Regulation
Released by the National Standardization Administration Committee
Table of contents
PrefaceⅠ
Introduction II
1 Scope 1
2 Normative reference documents 1
3 Terms and Definitions 1
4 Symbols and abbreviations3
5 Principle 3
6 Sample preparation for MA detection 4
6.1 Bacterial growth and AgNP treatment 4
6.2 Cell wall hydrolysis 5
6.3 Cell wall derivatization5
6.4 Gas Chromatography-Mass Spectrometry 6
7 Data analysis and interpretation of results 6
8 Detection methods for silver nanoparticle samples 7
Appendix A (Informative) Gas Chromatography-Mass Spectrometry 8
Appendix B (Informative) Internal Standard 9
Appendix C (informative) Laboratory preparation method of silver nanoparticle reference material 10
Appendix D (informative) Preparation of silver nanoparticle suspension 11
Appendix E (informative) Processing of control conditions; positive control, negative control, bacterial adaptability control 12
Reference 13
Foreword
This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
This document is equivalent to ISO /T S16550.2014 "Nanotechnology uses muramic acid released by Staphylococcus aureus to detect silver nanoparticles."
"Efficacy of Particles", the document type was adjusted from ISO technical specifications to my country's guiding technical documents.
This document adds a chapter "Normative References".
This document has made the following minimal editorial changes.
---Add Chapter 7 "The standard deviation of three parallel samples is ±0.0128" to Table 2 of that chapter.
This document is proposed by the Chinese Academy of Sciences.
This document is under the jurisdiction of the National Nanotechnology Standardization Technical Committee (SAC/TC279).
This document was drafted by. Institute of Basic Medicine, Chinese Academy of Medical Sciences.
The main drafters of this document. Liu Jian, Xu Haiyan, and Wen Tao.
Introduction
The antibacterial activity of silver nanoparticles (AgNP) has been increasingly used in the biomedical field and many consumer products, such as medical devices.
Mechanical coating materials, orthopedic or dental implant materials, local excipients for wound repair, textiles and even washing machines, etc. [9]. The company produces a variety of tools
Silver nanoparticles with different physical properties have been used as antibacterial agents, but little attention has been paid to their side effects. In addition, the biological effects of nanoparticles in different environments
Availability may increase[2][3], resulting in impacts and risks to soil beneficial microorganisms, plants, animals and humans. Therefore, silver nanoparticles
The potency of granules should be assessed and classified based on their ultimate biological activity.
This document does not address the evaluation of silver nanomaterial properties.
Nanotechnology harnesses Staphylococcus aureus to release
Muramic acid detects the efficacy of silver nanoparticles
1 Scope
This paper describes a method using gas chromatography-mass spectrometry (GC-MS) to quantitatively evaluate the effects of silver nanoparticles on Staphylococcus aureus cells.
Methods for wall degradation and muramic acid release efficacy.
2 Normative reference documents
This document has no normative references.
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
The property of a compound or substance to kill bacteria (bactericidal) or inhibit bacterial growth (bacteriostatic).
3.2
A physical technique that can be used to detect the particle size distribution of small particles in suspension or polymers in solution.
3.3
gas chromatography-mass spectrometry gaschromatography-massspectrometry; GC-MS
A technique that combines gas chromatography and mass spectrometry for qualitative and quantitative analysis of volatile compounds in samples to be tested (see Appendix A).
3.4
Grampositivebacteria Grampositivebacteria
In Gram staining, bacteria that can be stained/not destained by organic solvents.
3.5
internal standardinternalstandard
A compound of known concentration added to a sample to aid in the qualitative identification and/or quantitative determination of the sample's components (see Appendix B).
[Source. ISO 20752.2014,3.2]
3.6
Limit of detection limitofdetection;LOD
The analyte in the test sample can be reliably detected but is not required to be quantified, as demonstrated by collaborative testing or other appropriate verification.
Minimum amount or concentration.
[Source. ISO 8196-1.2009,3.4.3]
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