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GB/T 8381.3-2023 English PDF

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GB/T 8381.3-2023: Determination of lincosamides in feeds - Liquid chromatography-tandem mass spectrometry
Status: Valid

GB/T 8381.3: Evolution and historical versions

Standard ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status	PDF
GB/T 8381.3-2023	English	189	Add to Cart	3 days [Need to translate]	Determination of lincosamides in feeds - Liquid chromatography-tandem mass spectrometry	Valid	GB/T 8381.3-2023
GB/T 8381.3-2005	English	359	Add to Cart	3 days [Need to translate]	Determination of lincomycin in feed	Obsolete	GB/T 8381.3-2005

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Basic data

Standard ID	GB/T 8381.3-2023 (GB/T8381.3-2023)
Description (Translated English)	Determination of lincosamides in feeds - Liquid chromatography-tandem mass spectrometry
Sector / Industry	National Standard (Recommended)
Classification of Chinese Standard	B46
Classification of International Standard	65.120
Word Count Estimation	10,197
Date of Issue	2023-08-06
Date of Implementation	2024-03-01
Older Standard (superseded by this standard)	GB/T 8381.3-2005
Issuing agency(ies)	State Administration for Market Regulation, China National Standardization Administration

GB/T 8381.3-2023: Determination of lincosamides in feeds - Liquid chromatography-tandem mass spectrometry

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 65.120 CCSB46 National Standards of People's Republic of China Replace GB/T 8381.3-2005 Determination of lincosamides in feed Liquid chromatography-tandem mass spectrometry Published on 2023-08-06 2024-03-01 Implementation State Administration for Market Regulation Released by the National Standardization Administration Committee

Foreword

This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents" Drafting. This document replaces GB/T 8381.3-2005 "Determination of Lincomycin in Feed". Compared with GB/T 8381.3-2005, except for the structure In addition to adjustments and editorial changes, the main technical changes are as follows. a) Changed the scope of application (see Chapter 1, Chapter 1 of the.2005 edition); b) The principle has been changed (see Chapter 4, Chapter 4 of the.2005 edition); c) Reagents or materials have been changed (see Chapter 5, Chapter 5 of the.2005 edition); d) Changes in instruments and equipment (see Chapter 6, Chapter 6 of the.2005 edition); e) The test procedures have been changed (see Chapter 8, Chapter 8 of the.2005 edition); f) Test data processing has been changed (see Chapter 9, 10.2 of the.2005 edition). Please note that some contents of this document may involve patents, and the issuing organization of this document does not assume the responsibility for identifying patents. This document is proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC76). This document was drafted by. Zhejiang University, Zhejiang Qinglian Food Co., Ltd. The main drafters of this document. Wang Fengqin, Wang Yizhen, Lu Zeqing, Feng Jie, Wang Tenghao, Liu Bojing, Cheng Yuanzhi, Jin Mingliang, Wang Xinxia, Du Huahua, Dan Meizhong, Zong Xin, Kai Lixia. The previous versions of this document and the documents it replaces are as follows. ---First published as GB/T 8381.3-2005 in.2005; ---This is the first revision. Determination of lincosamides in feed Liquid chromatography-tandem mass spectrometry

1 Scope

This document describes the liquid chromatography-tandem mass spectrometry determination of lincomycin, clindamycin, clindamycin phosphate, and pirinomycin in feed. method. This document applies to lincomycin, clindamycin, clindamycin in compound feeds, concentrated feeds, concentrate supplements and additive premixed feeds Determination of protein phosphate and pirinomycin. The detection limit of this document is 10μg/kg and the limit of quantification is 40μg/kg.

2 Normative reference documents

The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, the dated quotations For undated referenced documents, only the version corresponding to that date applies to this document; for undated referenced documents, the latest version (including all amendments) applies to this document. GB/T 6682 Specifications and test methods for water used in analytical laboratories GB/T 20195 Preparation of animal feed samples

3 Terms and definitions

There are no terms or definitions to be defined in this document.

4 Principles

The test substance in the sample was extracted with methanol-phosphate buffer solution, purified by a mixed strong cation exchange solid-phase extraction cartridge, and liquid chromatography- Detected by tandem mass spectrometer and quantified by external standard method.

5 reagents or materials

Unless otherwise specified, use only analytically pure reagents. 5.1 Water. GB/T 6682, Level 1. 5.2 Formic acid. chromatographically pure. 5.3 Methanol. chromatographically pure. 5.4 Acetonitrile. chromatographically pure. 5.5 Hydrochloric acid solution. Pipette 0.9mL hydrochloric acid, dilute to 100mL with water, and mix well. 5.6 Phosphate buffer solution (pH 8.0). Weigh 11.411g of dipotassium hydrogen phosphate trihydrate, add 430mL of water to dissolve, and use hydrochloric acid solution (5.5) Adjust pH to 8.0±0.1, dilute to 500mL with water, and mix well. 5.7 Methanol-phosphate buffer solution. Take 800mL of methanol (5.3), add.200mL of phosphate buffer solution (5.6), and mix well. 5.8 Formic acid solution I. Take 1mL of formic acid (5.2), dilute to 100mL with water, and mix well.

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