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Wheat and wheat flour - Gluten content - Part 2: Determination of wet gluten and gluten index by mechanical means
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Basic data Standard ID | GB/T 5506.2-2024 (GB/T5506.2-2024) | Description (Translated English) | Wheat and wheat flour - Gluten content - Part 2: Determination of wet gluten and gluten index by mechanical means | Sector / Industry | National Standard (Recommended) | Classification of Chinese Standard | B20 | Classification of International Standard | 67.060 | Word Count Estimation | 18,128 | Date of Issue | 2024-11-28 | Date of Implementation | 2025-06-01 | Older Standard (superseded by this standard) | GB/T 5506.2-2008 | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 5506.2-2024: Wheat and wheat flour - Gluten content - Part 2: Determination of wet gluten and gluten index by mechanical means ---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Wheat and wheat flour - Gluten content - Part 2.Determination of wet gluten and gluten index by mechanical means
Gluten Content of Wheat and Wheat Flour
Part 2.Instrumental determination of wet gluten and
Gluten Index
Wheat and wheat flour-Gluten content-
Part 2.Determination of wet gluten and gluten index by mechanical means
(ISO 21415﹘2.2015, MOD)
ICS 67.060
CCS B 20
National Standard of the People's Republic of China
Replaces GB/T 5506.2-2008
Published on 2024-11-28 Implemented on 2025-06-01
State Administration for Market Regulation
The National Standardization Administration issued
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting is required.
This document is Part 2 of GB/T 5506 “Gluten content of wheat and wheat flour”. GB/T 5506 has been published for the following
part.
Part 1.Determination of wet gluten by hand washing method;
Part 2.Instrumental determination of wet gluten and gluten index;
Part 3.Determination of dry gluten by oven drying method;
Part 4.Determination of dry gluten by rapid drying method. -
This document replaces GB/T 5506.2-2008 "Wheat and wheat flour gluten content Part 2.Instrumental determination of wet gluten"
Compared with GB/T 5506.2-2008, in addition to structural adjustments and editorial changes, the main technical changes are as follows.
Added the term and definition of gluten index (see 3.3); -
Added the determination principle of gluten index (see Chapter 4); -
The requirements for centrifuges in instruments and equipment have been changed (see 6.3, 6.3 of the.2008 edition); -
The sampling requirements have been changed (see Chapter 7, Chapter 7 of the.2008 edition); -
The sample preparation requirements have been changed (see Chapter 8, Chapter 8 of the.2008 edition); -
The operating steps have been changed (see 9.2, 9.5.4, 9.5.5, 9.6, 9.7, Chapter 9 of the.2008 edition); -
Added the calculation formula for wet gluten content (14% moisture basis) (see 10.2); -
Added the calculation formula of gluten index (see 10.3); -
The precision requirements have been changed (see Chapter 11, Chapter 11 of the.2008 edition); -
Deleted test report (see Chapter 12 of the.2008 edition). -
This document is modified to adopt ISO 21415﹘2.2015 "Wheat and wheat flour gluten content Part 2.Instrumental method for determination of wet gluten content"
Quantity and Gluten Index.
Compared with ISO 21415﹘2.2015, this document has the following structural adjustments.
Added Chapter 2 Normative References, and postponed other chapters accordingly; -
Deleted Chapter 11 Inspection Report of ISO 21415﹘2.2015.-
The technical differences between this document and ISO 21415﹘2.2015 and their reasons are as follows.
The definition of wheat flour (see Chapter 3) has been changed to directly quote GB/T 1355, which is more suitable for practical application;
The sampling requirements (see Chapter 7) have been changed, and GB/T 5491 has been used instead of ISO 24333, which is more in line with the current grain storage situation in my country.
of sampling;
The determination method of moisture content has been added (see Chapter 8). GB 5009.3 or GB/T 5497 is used to determine the moisture content.
Convenient for calculation of wet gluten content (14% moisture basis);
The calculation formula for wet gluten content (14% moisture basis) has been added (see 10.2), so that the results can be directly used in some national standards;
The precision requirements have been changed (see Chapter 11), the requirements for the critical range of the standard have been deleted, and the precision calculation has been re-performed.
More suitable for sample testing in my country;
The inspection report has been deleted to comply with the requirements of my country's inspection method standards. -
The following editorial changes were also made to this document.
The IS0 5725﹘6 in the precision calculation method has been deleted and replaced with the informative references GB/T 6379.1-2004 and
GB/T 6379.2-2004 replaces IS0 5725﹘1 and IS0 5725﹘2 (see Appendix C).
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed by the State Administration of Grain and Material Reserves.
This document is under the jurisdiction of the National Technical Committee on Standardization of Cereals and Oils (SAC/TC 270).
This document was drafted by. National Grain and Material Reserves Administration Scientific Research Institute, Anhui Grain and Oil Product Quality Supervision and Inspection Station, Shandong Grain and Oil Product Quality Supervision and Inspection Station,
Oil Testing Center, Beijing Food Inspection Institute (Beijing Food Safety Monitoring and Risk Assessment Center), Dezhou Food Safety and Security
Center [Dezhou Grain Quality Inspection (Center) Station], Ningxia Hui Autonomous Region Grain and Oil Product Quality Inspection Center, Xinjiang Academy of Agricultural Sciences,
Hebei Grain and Oil Quality Inspection and Information Service Center, Shenzhen Shenliang Quality Inspection Co., Ltd., and Anhui Zhengyu Flour Co., Ltd.
The main drafters of this document are. Hong Yu, Sun Hui, Chang Liu, Duan Xiaoliang, Hu Bin, Wang Ruijie, Li Hui, Wu Haibin, Xing Xiaoting, Zhou Guiying,
Yin Huijun, Zhang Chun'e, Liu Hui, Sang Wei, Fan Ou, Ma Hang, Wang Jing, Xiao Jianwen, Zhang Dan, Zhang Wenzhan.
The previous versions of this document and the documents it replaces are as follows.
First published in.2008 as GB/T 5506.2-2008;
This is the first revision. -
Introduction
GB/T 5506 adopts the ISO 21415 series of international standards. The settings of each part are consistent with the international standards and consist of 4 parts.
Part 1.Determination of wet gluten by hand washing method;
Part 2.Instrumental determination of wet gluten and gluten index;
Part 3.Determination of dry gluten by oven drying method;
Part 4.Determination of dry gluten by rapid drying method. -
The purpose of GB/T 5506.1 is to determine the wet gluten content of wheat and wheat flour and describes the method for determining the wet gluten content by hand washing.
The purpose of GB/T 5506.2 is to determine the wet gluten content and gluten index of wheat and wheat flour, and to describe the instrumental determination of the wet gluten content and gluten index of wheat and wheat flour.
GB/T 5506.3 and GB/T 5506.4 are intended to determine the dry gluten content of wheat and wheat flour.
GB/T 5506.3 describes the method of determining the dry gluten content in an oven after determining the wet gluten content in GB/T 5506.1 and GB/T 5506.2.
GB/T 5506.4 describes the determination of wet gluten by GB/T 5506.1 and GB/T 5506.2, and the determination of dry gluten by an electric heating dryer.
Gluten content method.
Gluten Content of Wheat and Wheat Flour
Part 2.Instrumental determination of wet gluten and
Gluten Index
1 Scope
This document describes a method for the instrumental determination of the wet gluten content and gluten index of wheat and wheat flour.
This document applies to the testing of wet gluten content and gluten index of whole wheat flour and wheat flour.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, the latest version (including all amendments) shall apply.
in this document.
GB/T 1355 Wheat Flour
GB 5009.3 National Food Safety Standard Determination of Water Content in Food
GB/T 5491 Sampling and subsampling methods for grain and oilseed inspection
GB/T 5497 Grain and oilseeds - Determination of moisture content
GB/T 5506.1 Gluten content of wheat and wheat flour Part 1.Determination of wet gluten by hand washing method
3 Terms and definitions
The terms and definitions defined in GB/T 1355 and the following apply to this document.
3.1
whole wheat flour
Whole wheat flour is made from whole wheat grains.
Note. Contains wheat germ and bran.
3.2
wet gluten
A viscoelastic substance that is hydrated but not dehydrated.
Note. It is mainly composed of two hydrated protein components of wheat (glutenin and prolamin).
3.3
Gluten index
The percentage of gluten mass that does not pass through the sieve to the total wet gluten mass after centrifugation.
4 Principle
Whole wheat flour or wheat flour is added with sodium chloride solution to make dough. The dough is washed with sodium chloride solution to remove starch and other substances in the dough.
The excess water is removed by centrifugation, and the gluten is separated and weighed, which is the wet gluten. After the wet gluten is centrifuged, part of the wet gluten is
The percentage of the mass of gluten that passes through the sieve holes and remains on the sieve to the mass of the total wet gluten is the gluten index.
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