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GB/T 5009.208-2016 English PDF

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GB/T 5009.208-2016: Determination of biogenic amine in foods

GB/T 5009.208: Evolution and historical versions

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GB/T 5009.208-2016English219 Add to Cart 3 days [Need to translate] Determination of biogenic amine in foods GB/T 5009.208-2016
GB/T 5009.208-2016English219 Add to Cart 3 days [Need to translate] Determination of biogenic amine in foods GB/T 5009.208-2016
GB/T 5009.208-2008English279 Add to Cart 3 days [Need to translate] Determination of biogenic amine in foods Obsolete GB/T 5009.208-2008

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Basic data

Standard ID GB/T 5009.208-2016 (GB/T5009.208-2016)
Description (Translated English) Determination of biogenic amine in foods
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard X09
Word Count Estimation 11,166
Issuing agency(ies) National Health and Family Planning Commission of the People's Republic of China, State Food and Drug Administration

GB/T 5009.208-2008: Determination of biogenic amine in foods

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of biogenic amine in foods ICS 67.040 C53 National Standards of People's Republic of China Determination of levels of biogenic amines Posted 2008-11-21 2009-03-01 implementation People's Republic of China Ministry of Health Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. This standard is proposed and administered by the People's Republic of China Ministry of Health. This standard by the People's Republic of China Ministry of Health is responsible for interpretation. This standard was drafted. Chinese Center for Disease Control Nutrition and Food Safety. Participated in the drafting of this standard. Beijing Center for Disease Control and Prevention, Yantai University, Henan Drug Control. The main drafters of this standard. Wu Yongning, Zhao Yunfeng, Li Zhijun, Lu Bin, Li Jingguang, Zhang Lei, Shao Bing, Miao Hong. Determination of levels of biogenic amines

1 Scope

This standard specifies the foods tryptamine, β- phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine content determination. This standard applies to alcohol (wine, beer, wine, etc.), spices (vinegar, soy sauce, etc.), seafood (fish and their products, and shrimp Products), meat and dairy products Determination of biogenic amines. The standard quantitative limit. a) wine, vinegar condiment. tryptamine 3μg/kg, β- phenylethylamine 10μg/kg, putrescine 3μg/kg, cadaverine 7μg/kg, histamine 10μg/kg, tyramine 10μg/kg, spermidine 5μg/kg, spermine 7μg/kg. b) aquatic products, meat, dairy products and beans as raw material to seasoning. tryptamine 15μg/kg, β- phenylethylamine 40μg/kg, putrescine 15μg/kg, cadaverine 35μg/kg, histamine 50μg/kg, tyramine 25μg/kg, spermidine 25μg/kg, spermine 35μg/kg. Principle 2 With 1,7-amino-heptane as an internal standard, with 5% trichloroacetic acid solution to extract, shaking extraction with hexane to remove fat, through trichlorosilane Alkyl - n-butanol (1 + 1) liquid-liquid extraction purified to dansyl chloride derived agents, 60 ℃ derivative 30min, HPLC C18 column Separation, UV detector, internal standard.

3 Reagents and materials

Unless otherwise specified in this standard reagents used were of analytical grade. 3.1 methanol (CH4O). chromatography. 3.2 acetone (C3H6O). chromatography. 3.3 ether (C4H10O). redistilled. 3.4 n-butanol (C4H10O). 3.5 chloroform (CHCl3). 3.6 n-hexane (C6H14). chromatography. 3.7 sodium glutamate (C5H8NNaO4). 3.8 sodium bicarbonate (NaHCO3). 3.9 Sodium chloride (NaCl). 3.10 Sodium hydroxide (NaOH). 3.11 hydrochloric acid (HCl). 3.12 TCA (C2HCl3O2). 3.13 histamine hydrochloride (histaminedihydrochloride, C5H9N3 · 2HCl) standards (purity > 99%, when calculating conversion off Hydrochloride, C5H9N3/C5H9N3 · 2HCl = 155/184). 3.14 β- phenylethylamine (β-phenylethylamine, C8H11N) standards (purity > 98%). 3.15 tyramine hydrochloride (tyraminehydrochloride, C8H12ClNO) standards (purity > 99%, when calculating conversion off hydrochloride, C8H11NO/C8H11NO · HCl = 137/174). 3.16 putrescine (putrescine, C4H12N2) standards (purity > 98%). 3.17 cadaverine (cadaverine, C5H14N2) standards (purity > 98%). 3.18 tryptamine (tryptaminehydrochloride, C10H12N2) standards (purity > 99%). 3.19 spermidine (spermidine, C7H19N3) standards (purity > 97%).

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