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GB/T 45589-2025 English PDF

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GB/T 45589-2025: Detection and identification of Tobamovirus fructirugosum
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GB/T 45589-2025English379 Add to Cart 4 days [Need to translate] Detection and identification of Tobamovirus fructirugosum Valid GB/T 45589-2025

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Basic data

Standard ID GB/T 45589-2025 (GB/T45589-2025)
Description (Translated English) Detection and identification of Tobamovirus fructirugosum
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 65.020.20
Word Count Estimation 18,194
Date of Issue 2025-04-25
Date of Implementation 2025-11-01
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 45589-2025: Detection and identification of Tobamovirus fructirugosum

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 65.020.20 CCSB16 National Standard of the People's Republic of China Quarantine and identification method of tomato brown wrinkled fruit virus Released on 2025-04-25 2025-11-01 Implementation State Administration for Market Regulation The National Standardization Administration issued

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents" Drafting. Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents. This document is proposed and coordinated by the National Technical Committee on Plant Quarantine Standardization (SAC/TC271). This document was drafted by. China Institute of Inspection and Quarantine, Beijing Academy of Agricultural and Forestry Sciences, Fuzhou Customs Technical Center, Guangzhou Customs Technical Center Center, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, and Shandong Agricultural University. The main drafters of this document are. Zhang Yongjiang, Qiu Yanhong, Zhao Zhenxing, Shen Jianguo, Feng Lixia, Zhou Xueping, Li Xiangdong, Dong Zheng, and Yang Xiuling. Quarantine and identification method of tomato brown wrinkled fruit virus

1 Scope

This document describes serological and molecular methods for the detection of tomato brown crinkle fruit virus. This document applies to the quarantine identification of tomato brown wrinkled fruit virus.

2 Normative references

The contents of the following documents constitute essential clauses of this document through normative references in this document. For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to This document. GB/T 6682 Specifications and test methods for water used in analytical laboratories

3 Terms and definitions

There are no terms or definitions that require definition in this document.

4 Tomato brown fruit virus classification information

Chinese name. Tomato brown wrinkled fruit virus Abbreviation.ToBRFV Taxonomic status. Martelivirales, Virgaviridae, Tobacco mosaic virus (Tobamovirus). Additional information on Tomato Brown Fruit Virus is provided in Appendix A.

5 Principles of the method

The serological characteristics and genomic characteristics of tomato brown wrinkle virus are the basis for quarantine identification of the virus. And dual real-time fluorescence RT-PCR detection method; through the effective combination of these methods, it is determined whether the sample carries tomato brown wrinkle virus. 6 Reagents, instruments, equipment and utensils 6.1 Reagents Unless otherwise specified, all reagents are of analytical grade and the test water should comply with the relevant provisions of Grade 3 water in GB/T 6682. DAS-ELISA reagents should comply with B.1 in Appendix B. The colloidal gold immunochromatographic test strip reagents shall comply with C.1 in Appendix C.

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