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GB/T 43160-2023 English PDF

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GB/T 43160-2023: Detection and identification of Erwinia amylovora
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Basic data

Standard ID GB/T 43160-2023 (GB/T43160-2023)
Description (Translated English) Detection and identification of Erwinia amylovora
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 65.020.20
Word Count Estimation 14,148
Date of Issue 2023-09-07
Date of Implementation 2024-04-01
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 43160-2023: Detection and identification of Erwinia amylovora

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 65.020.20 CCSB16 National Standards of People's Republic of China Quarantine and identification methods of Erwinia amylovora Published on 2023-09-07 2024-04-01 Implementation State Administration for Market Regulation Released by the National Standardization Administration Committee

Foreword

This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents" Drafting. Please note that some content in this document may be subject to patents. The publisher of this document assumes no responsibility for identifying patents. This document is proposed and coordinated by the National Technical Committee for Phytosanitary Standardization (SAC/TC271). This document was drafted by. Chinese Academy of Inspection and Quarantine, Tarim University, Biosafety Center of Chinese Academy of Inspection and Quarantine in Sanya, Shanghai Maritime Customs, Guangzhou Customs Technology Center, Urumqi Customs Technology Center, Tianjin Binhai New Area Agricultural Service Center, Changji Agricultural Technology Promotion Center Center, Yining Customs Technology Center, Nanjing Agricultural University. The main drafters of this document. Zhao Wenjun, Tian Qian, Zhang Wangbin, Ma Yunlong, Yi Jianping, Wei Shuang, Wang Chong, Zhang Xiaoju, Li Leshu, Wang Chaoyang, Meng Ru, Lu Ping, Hu Baishi. Quarantine and identification methods of Erwinia amylovora

1 Scope

This document describes the isolation, culture, immunology and molecular biology detection methods of Erwinia amylovora. This document is applicable to the quarantine and identification of fire blight bacteria transmitted to plants, rootstocks, scions and young fruits of Rosaceae plants such as pears and apples, as well as the disease Field survey and monitoring of damage.

2 Normative reference documents

This document has no normative references.

3 Terms and definitions

There are no terms or definitions to be defined in this document. 4.Basic information about Erwinia amylovora Scientific name. Erwiniaamylovora (Burril,1883)Winslowetal.,1920 Transmission routes. infected plant propagation materials (including seedlings, rootstocks and scions), insects, wind, rain, and contaminated packaging materials and transportation Various natural factors and man-made factors such as transportation tools can cause the spread of pear fire blight. Among them, the infected plant propagation material is pear fire blight The main route of long-distance transmission. See Appendix A for additional information on Erwinia amylovora.

5 Method Principles

Immunological detection based on the specific reaction between E. amylovora and antibodies; based on the specific DNA sequence of E. amylovora Carry out molecular biology testing; conduct testing of pathogenic bacteria based on their culture traits, biological characteristics, host range and harmful symptoms, etc. Isolation, culture, identification and pathogenicity determination. 6 Instruments, equipment and main reagents 6.1 Instruments, equipment and tools Clean bench, constant temperature incubator, shaker, ultra-low temperature refrigerator, conventional refrigerator, high pressure sterilizer, constant temperature water bath, small centrifuge, high speed Centrifuge, PCR machine, real-time fluorescence PCR machine, electrophoresis machine, horizontal electrophoresis tank, gel imaging system, Biolog microplate, fully automatic microbiology Identification instrument (Biolog), ice maker, electronic balance, vortex oscillator, micro-injector, etc. 6.2 Main reagents 2× PCR reaction master mix, 2× fluorescence PCR reaction master mix, etc., buffer and culture medium are prepared according to Appendix B, unless otherwise specified In addition, all reagents are of analytical grade.

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