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GB/T 42470-2023 English PDF

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GB/T 42470-2023: Nanotechnologies - Assessment of nanomaterial toxicity using dechorionated zebrafish embryo
Status: Valid

Standard ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status	PDF
GB/T 42470-2023	English	359	Add to Cart	4 days [Need to translate]	Nanotechnologies - Assessment of nanomaterial toxicity using dechorionated zebrafish embryo	Valid	GB/T 42470-2023

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Basic data

Standard ID	GB/T 42470-2023 (GB/T42470-2023)
Description (Translated English)	Nanotechnologies - Assessment of nanomaterial toxicity using dechorionated zebrafish embryo
Sector / Industry	National Standard (Recommended)
Classification of Chinese Standard	C04
Classification of International Standard	07.120
Word Count Estimation	18,155
Date of Issue	2023-03-17
Date of Implementation	2023-10-01
Issuing agency(ies)	State Administration for Market Regulation, China National Standardization Administration

GB/T 42470-2023: Nanotechnologies - Assessment of nanomaterial toxicity using dechorionated zebrafish embryo

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS07:120 CCSC04 National Standards of People's Republic of China Nanotechnology based on zebrafish embryos Toxicity Evaluation of Nanomaterials (ISO /T S22082:2020, MOD) Released on 2023-03-17 2023-10-01 implementation State Administration for Market Regulation Released by the National Standardization Management Committee

Preface I Introduction II 1 Scope 1 2 Normative references 1 3 Terms and Definitions 1 4 Abbreviations 2 5 material 2 5:1 Organisms (zebrafish, Daniorerio) 2 5:2 Stock solution 3 5:3 Positive control 3 6 equipment 3 6:1 Technical equipment 3 6:2 Analytical Instruments 3 7 Step 4 7:1 Breeding 4 7:2 Spawn stimulation 4 7:3 Embryo rupture 5 7:4 Preparation of nanomaterial stock solution 6 7:5 Test concentration 6 7:6 Dispersion of Nanomaterials 6 7:7 Positive control chemical: 3,4-dichloroaniline6 7:8 Test method 7 7:9 Data Analysis 8 8 Test report 8 8:1 Test steps 8 8:2 Information contained in the report8 Appendix A (informative) mechanical membrane rupture method 10 Appendix B (Informative) Zebrafish Embryo Spawning Step 11 Appendix C (Informative) Result Verification 12 Reference 13

foreword

This document is in accordance with the provisions of GB/T 1:1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules for Standardization Documents" drafting: This document is modified to adopt ISO /T S22082:2020 "Nanotechnology-based Toxicity Evaluation of Nanomaterials in Zebrafish Embryos", document category The type is adjusted from the ISO technical specification to the national standard of our country: The technical differences between this document and ISO /T S22082:2020 and their reasons are as follows: ---The original picture 1 does not conform to the morphological characteristics of the female fish and the male fish are small in the description in 5:1, so the picture of the male and female zebrafish in Figure 1 has been changed slice (see Figure 1); --- Added notes in 5:2, citing national standards to explain the quality of purified water (see 5:2); ---In 5:2, the reference to the later part of the stock solution configuration method is added (see 5:2); ---The photoperiod in 6:1:2 does not conform to conventional experiments and domestic practices, so the relevant national standards are quoted and the value of photoperiod is changed (see 6:1:2); --- According to domestic practice, "analytical purity" is added in 7:3:2 to define the purity level of chemicals (see 7:3:2); ---The test steps in 7:8:3 need to be carried out sequentially, so the "-" symbol before each step has been changed, and it has been changed to an English letter number (see 7:8:3): Please note that some contents of this document may refer to patents: The issuing agency of this document assumes no responsibility for identifying patents: This document was proposed by the Chinese Academy of Sciences: This document is under the jurisdiction of the National Nanotechnology Standardization Technical Committee (SAC/TC279): This document was drafted by: Capital Medical University, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences: The main drafters of this document: Sun Zhiwei, Li Yang, Duan Junchao, Chen Yueyue, Li Yanbo, Xu Haiyan, Meng Jie:

Introduction

Fish test is an important means widely used to evaluate the toxicity of chemicals in water environment: However, using vertebrates including fish There are animal welfare concerns about testing chemicals: Testing with fully developed juveniles can cause significant discomfort to the animals (e:g: using OECDTG203), the use of early-life fish embryos rather than adults or juveniles for testing is a more animal welfare alternative method: Nanotechnology is playing an active role in many fields, but people still worry about the potential harm of nano products to the environment: economic cooperation The Organization for Operations and Development's (OECD) test guideline for the assessment of acute toxicity using fish embryos (see OECDTG236) states that some chemicals may This method is not suitable for testing, such as macromolecular structure substances with molecular weight ≥ 3kDa and substances that cause incubation delay: fish embryo The outermost acellular envelope of the fetus, its barrier function will hinder the evaluation of the biological activity of chemicals or nanomaterials, and it is currently impossible to predict which nanomaterials Rice materials are affected by this barrier: Toxicity assessments using ovariectomized embryos, although not providing direct ecotoxicological information, may Can help to better identify potentially hazardous nanomaterials: Therefore, researchers from all over the world have developed many methods to remove biological egg membranes of early stage zebrafish embryos[4][5]: There are two ways to remove the egg membrane of embryos: enzymatic membrane rupture and mechanical membrane rupture: mechanical membrane rupture Compared with the traditional method, the enzymatic membrane disruption method has the following advantages (see Appendix A): it saves time and labor, avoids mechanical damage to the embryo, and at the same time provides a large A large number of membrane-ruptured embryos for high-throughput testing: However, one disadvantage of enzymatic membrane disruption is that changes in pronase activity may affect egg membrane removal: success rate of removal: Many research groups have used egg-thinned embryos to evaluate the toxicity of chemicals and nanomaterials [6]-[9], but the methods have not yet been developed: Fully normalized [10]–[13]: Ovulated zebrafish embryos can be used as an alternative system to other vertebrate systems for detecting potential hazards of nanomaterials: with high With the continuous optimization of animal model toxicity tests, the demand for alternative test methods continues to increase: Zebrafish in early life stages (independent photo Before feeding, that is, within 120h after fertilization) is a very good surrogate model for in vivo toxicity test[23]-[28]: Compared with other animal models, the zebrafish The model has many advantages, including relative ease of raising and breeding, strong fecundity (in vitro fertilization, a female fish can produce:200-300 embryos), Short developmental cycle (approximately 3 months to adulthood), availability of genomic resources (complete zebrafish genome sequence), and genetic correlation with humans Similarity: Comparison with the human genome shows that about 70% of human genes have at least one homologous gene in zebrafish[14], so zebrafish Horsefish have been increasingly used in the evaluation of chemical toxicity: This document provides a method for toxicity assessment using ovariectomized zebrafish embryos and recommends an optimal method for removing the egg membranes of zebrafish embryos: The procedure was discussed, and the advantages of using oviembranous embryos for fish toxicity assessment were discussed: Nanotechnology based on zebrafish embryos Toxicity Evaluation of Nanomaterials

1 Scope

This document specifies a method for rapid assessment of the toxicity of nanomaterials (early life stages of fish, 0HPF~120HPF), including Detailed protocol for removing egg membranes and a complete protocol for evaluating nanomaterial toxicity using egg membrane-depleted zebrafish embryos: The focus of this document is to test the Toxicity of nanomaterials: This document applies to the toxicity testing of commercial and non-commercial nanomaterials:

2 Normative references

The contents of the following documents constitute the essential provisions of this document through normative references in the text: Among them, dated references For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document: GB/T 6682-2008 Analytical laboratory water specifications and test methods ISO /T S12805 Nanotechnologies-Materialsspecifi- Note: GB/T 37156-2018 Nanotechnology Material Specification Guidelines for Nano-object Characteristics (ISO /T S12805:2011, IDT) ISO /T R13014 Guidelines for Characterization of Physicochemical Properties of Nanotechnology Nanomaterials Before Toxicological Evaluation (Nanotechnologies-Guid- ment) Note: GB/T 39261-2020 Guidelines for Characterization of Physicochemical Properties of Nanotechnology Nanomaterials Before Toxicological Evaluation (ISO /T R13014:2012, IDT) ISO /T S17200 Nanotechnology powder nanoparticle characterization and measurement (Nanotechnology-Nanoparticlesin ISO /T R18196 Nanotechnologies-Measurement Matrix for Characterization of Nanoobjects Note: GB/T 41204-2021 Nanotechnology Nano-object Characterization Measurement Technology Matrix (ISO /T R18196:2016, MOD) ISO /T S80004-1 Nanotechnology Terminology Part 1: Core Terms (Nanotechnologies-Vocabulary-Part 1: Core terms) Note: GB/T 30544:1-2014 Nanotechnology Terminology Part 1: Core Terms (ISO /T S80004-1:2010, IDT) ISO /T S80004-2 Nanotechnology Terminology Part 2: Nanoobjects (Nanotechnologies-Vocabulary-Part 2: Nano-objects) OECDTG236: Fish Embryo Acute Toxicity Test OECD Guidelines for Testing Chemicals Part 2: OECD Press, Brazil

3 Terms and Definitions

ISO /T S12805, ISO /T R13014, ISO /T S17200, ISO /T S80004-1, ISO /T S80004-2, OECDTG236

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