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GB/T 40974-2021: Quality evaluation methods of the nucleic acid
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Quality evaluation methods of the nucleic acid
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GB/T 40974-2021
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Basic data | Standard ID | GB/T 40974-2021 (GB/T40974-2021) | | Description (Translated English) | Quality evaluation methods of the nucleic acid | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C40 | | Word Count Estimation | 11,196 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 40974-2021: Quality evaluation methods of the nucleic acid---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quality evaluation methods of the nucleic acid
ICS 07.080
CCSC40
National Standards of People's Republic of China
Nucleic acid sample quality evaluation method
Released on 2021-11-26
2022-06-01 implementation
State Administration for Market Regulation
Issued by the National Standardization Management Committee
Table of contents
Foreword Ⅰ
1 Scope 1
2 Normative references 1
3 Terms and definitions 1
4 Quality evaluation method 3
4.1 DNA sample 3
4.1.1 DNA concentration 3
4.1.2 DNA purity 4
4.1.3 DNA integrity 4
4.2 RNA sample 5
4.2.1 RNA concentration 5
4.2.2 RNA purity 5
4.2.3 RNA integrity 5
Reference 7
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents.
This document was proposed and managed by the National Standardization Technical Committee for Biological Samples (SAC/TC559).
Drafting organizations of this document. Shanghai Biochip Co., Ltd., China Institute of Metrology, Shanghai Xinchao Biotechnology Co., Ltd., Shanghai
Renji Hospital Affiliated to School of Medicine, Jiaotong University, Taizhou Hospital, Zhejiang Province, Guangdong Provincial Hospital of Traditional Chinese Medicine (The Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine), National
Institute of Science and Technology of the Health Commission, Shanghai Pharmaceutical Clinical Research Center Co., Ltd., Agilent Technologies (China) Co., Ltd., Perkinel
Mo Enterprise Management (Shanghai) Co., Ltd.
The main drafters of this document. Gao Hengjun, Wang Jing, Zhang Xiaoyan, Xu Jingman, Kang Xiaonan, Lin Aifen, Chen Qubo, Gao Huafang, Du Lili,
Yan Weihua, Liu Xin, Ruan Liangliang, Hu Xingyu, Niu Chunyan, Lu Xinyi, Sun Jing, Peng Yuping, Huang Yu, Zhang Jia.
Nucleic acid sample quality evaluation method
1 Scope
This document describes the quality assessment methods for the concentration, purity and completeness of nucleic acid samples.
This document is applicable to the quality evaluation of nucleic acid samples from human, animal, plant, and microbial sources.
This document is not applicable to the quality evaluation of small molecule nucleic acid samples.
2 Normative references
There are no normative references in this document.
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Nucleotide
The phosphate ester of nucleoside is the basic unit of nucleic acid.
Note. The connection position is different, there are 2'-nucleotide (nucleoside-2'-phosphate), 3'-nucleotide (nucleoside-3'-phosphate), 5'-nucleotide (nucleoside-5 '-Phosphoric acid) three kinds.
3.2
Nucleic acid
A class of biological macromolecules connected by nucleotides or deoxynucleotides through 3',5'-phosphodiester bonds. Very important creatures
The function is mainly to store heritage information and transmit genetic information. Including two types of ribonucleic acid and deoxyribonucleic acid.
3.3
Deoxyribonucleic acid deoxyribonucleic acid; DNA
Biological macromolecules with genetic information. It consists of four main deoxynucleotides (deoxy adenine monophosphate dAMP, deoxy monophosphate
Guanine dGMP, deoxycytosine monophosphate dCMP and deoxythymine monophosphate dTMP) are connected by 3',5'-phosphodiester bonds.
become.
Note. Their composition and arrangement are different, showing different biological functions, such as. coding function, copy and transcription regulation functions.
3.4
Ribonucleic acid;RNA
A type of nucleic acid. A polymer composed of nucleotides connected by 3',5'-phosphodiester bonds.
Note. Different types of RNA have different chain lengths and perform various biological functions. For example, RNA related to protein biosynthesis has messenger RNA (mes-
sengerRNA, mRNA), transfer RNA (transferRNA, tRNA) and ribosomal RNA (ribosomeRNA, rRNA); and post-transcription
Work-related RNAs include small nuclear RNA (smalnuclearRNA, snRNA), small nucleolar RNA (smalnucleolarRNAs, snoRNAs); and
The RNA related to biological regulation includes microRNAs (miRNA), small interfering RNA (smalinterferingRNA, siRNA) and so on.
3.5
Plasmid
A self-replicating circular double-stranded DNA molecule in bacterial cells that can stably exist outside the chromosomes and be passed on to the offspring.
It is generally not integrated into the host chromosome.
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