Search result: GB/T 40226-2021
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Detection of environmental microbial metagenome - High throughput sequencing
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GB/T 40226-2021
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| Standard ID | GB/T 40226-2021 (GB/T40226-2021) | | Description (Translated English) | | | Sector / Industry | National Standard (Recommended) |
GB/T 40226-2021
Detection of environmental microbial metagenome-High throughput sequencing
ICS 07.080
CCSA40
National Standards of People's Republic of China
High-throughput sequencing method for environmental microbial metagenomic detection
Released on 2021-05-21
2021-12-01 implementation
State Administration of Market Supervision and Administration
Issued by the National Standardization Management Committee
Table of contents
Foreword Ⅲ
1 Scope 1
2 Normative references 1
3 Terms and definitions 1
4 Abbreviations 2
5 Principle 2
6 Test condition 2
7 Reagent 2
8 Instruments and equipment 2
9 Sample 3
10 Test Step 3
11 Test report 4
12 Quality Control 5
Appendix A (normative) Methods of collecting, storing, and transporting stool samples 6
Appendix B (Normative) Methods of soil sample collection, preservation, and transportation 7
Appendix C (Normative) Methods of Water Sample Collection, Preservation, and Transportation 8
Appendix D (Informative) Sample Information Sheet 9
Appendix E (Informative) The completeness map and quality level classification of DNA samples 10
Appendix F (Informative) Reference Database 11
Reference 12
Preface
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents.
This document was proposed and managed by the National Standardization Technical Committee for Biochemical Testing (SAC/TC387).
Drafting organizations of this document. Shenzhen Huada Life Science Research Institute, Shenzhen Huada Gene Technology Co., Ltd., Shenzhen Life Science and Technology Research Institute
Capital Alliance, Institute of Biology, China Academy of Testing Technology, Shenzhen Huada Clinical Laboratory Center.
The main drafters of this document. Chen Bing, Xiao Liang, Zhong Huanzi, Li Junhua, Li Qianyi, Jia Huijue, Jiang Huayan, Zhou Lihua, Tang Meifang, Wu Hao,
Li Taosha, Zhou Yuan, Zhong Hongbin.
High-throughput sequencing method for environmental microbial metagenomic detection
1 Scope
This document describes the terms and definitions, principles, test conditions,
Reagents, equipment, samples, test procedures, test reports and quality control requirements.
This document is suitable for the detection of environmental microbial metagenomics using high-throughput sequencing methods.
2 Normative references
The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations
Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
This document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB 19489 Laboratory Biosafety General Requirements
GB/T 35537-2017 High-throughput gene sequencing results evaluation requirements
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Environment
The surrounding world relative to a central thing.
Note. In this document, it refers specifically to the human (animal) environment and the natural environment. The human (animal) environment includes but is not limited to the oral cavity, skin, reproductive tract, intestinal tract, etc.; natural
The environment includes but is not limited to air, water, soil, sediment, etc.
3.2
Relative abundance
The relative proportion of a specific kind of microorganisms in the environmental microbial community.
Note. Usually expressed as a percentage.
3.3
Microbial metagenomics microbialmetagenome
Take the entire microbial community in a specific environment as the research object, without separation and cultivation, directly extract all the microbial genes obtained
Group DNA.
Note. It can be used to analyze its genetic information, species classification, system evolution, gene function and metabolic network, etc.
3.4
Qualityofbasecaling
Evaluate the probability of accurate base recognition.
Note. Abbreviated as Q, usually expressed as a numerical value. The base recognition quality value is negatively correlated with the base recognition error rate, and the two follow a logarithmic function relationship. Base recognition quality
The higher the value, the lower the error rate.
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