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Identification of plant varieties - MNP marker method
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GB/T 38551-2020
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Basic data | Standard ID | GB/T 38551-2020 (GB/T38551-2020) | | Description (Translated English) | Identification of plant varieties - MNP marker method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B04 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 330,332 | | Date of Issue | 2020-03-06 | | Date of Implementation | 2020-10-01 | | Quoted Standard | GB/T 3543.2; GB/T 6882; GB/T 19557.1; NY/T 2594 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | | Summary | This standard specifies the principles, reagents or materials, instruments and equipment, determination steps and result analysis of the polynucleotide polymorphism (MNP) labeling method for plant variety identification. This standard applies to rice (Oryza sativa L.), corn (Zea mays L.), soybean [Glycine max (Linn.) Merr.], cotton (Gossypium spp.), peanut (Arachis hypogaea L.), millet (Setaria italica ), watermelon (Citrullus lanatus), melon (Cucumis melo L.), cucumber (Cucumis sativus L.), wormwood (A | GB/T 38551-2020: Identification of plant varieties - MNP marker method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Identification of plant varieties--MNP marker method
ICS 65.020.01
B04
National Standards of People's Republic of China
Plant variety identification MNP marker method
Released on 2020-03-06
2020-10-01 implementation
State Administration of Market Supervision and Administration
Issued by the National Standardization Management Committee
Contents
Foreword Ⅲ
1 Scope 1
2 Normative references 1
3 Terms and definitions 1
4 Principle 2
5 Reagents or materials 2
6 Equipment 2
7 Measurement step 2
7.1 Operational requirements 2
7.2 Sampling 2
7.3 DNA extraction 2
7.4 Multiplex PCR amplification and library construction 2
7.5 High-throughput sequencing 2
7.6 Sequencing data quality control 3
8 Results analysis 3
8.1 Calculation of results 3
8.2 Judgment of results 3
Appendix A (Normative Appendix) MNP Marker Primer 5
A.1 Rice MNP marker primer 5
A.2 Maize MNP marker primer 35
A.3 Soybean MNP primer 63
A.4 Cotton MNP marker primer 78
A.5 Peanut MNP primer 109
A.6 Millet MNP labeled primer 124
A.7 Watermelon MNP marker primer 148
A.8 Melon MNP primer 171
A.9 Cucumber MNP labeled primer 192
A.10 Wormwood MNP primer 208
A.11 Tomato MNP primer 218
A.12 Pepper MNP primer 235
A.13 Chinese cabbage MNP marker primer 260
A.14 Longan MNP primer 275
A.15 Litchi MNP primer 294
A.16 Kiwi MNP primer 312
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by China National Institute of Standardization.
This standard was drafted by. Jianghan University, Wuhan Mingle Biotechnology Co., Ltd., China National Institute of Standardization, Ministry of Agriculture and Rural Science and Technology Development
Center, Beijing Technology and Business University, Vegetable Research Center of Beijing Academy of Agriculture and Forestry Sciences, Hunan Hybrid Rice Research Center, South China Agricultural University, Zhangjiakou
Academy of Agricultural Sciences, Shenzhen Huada Zhizao Technology Co., Ltd.
The main drafters of this standard. Peng Hai, Fang Zhiwei, Li Lun, Ma Aijin, Zhou Junfei, Wen Changlong, Li Tiantian, Tang Hao, Chen Hong, Cui Yehan, Zhang Jianan,
Jia Yingmin, Xu Na, Song Shufeng, Hu Meixia, Fu Xiqin, Zhao Zhihai, Liang Yong, Xu Zhenjiang, Gao Lifen, Chen Lihong, Han Ruixi, Zhang Die, Zhang Jing,
Yu Jinwen.
Plant variety identification MNP marker method
1 Scope
This standard specifies the principles, reagents or materials, instruments and equipment, and determination steps of the polynucleotide polymorphism (MNP) labeling method for plant variety identification
Steps, results analysis.
This standard applies to rice (Oryzasativa L.), corn (Zeamays L.), soybean [Glycinemax (Linn.) Merr.], Cotton
Flowers (Gossypiumspp.), Peanuts (Arachishypogaea L.), millet (Setariaitalica), watermelon (Citrulluslanatus), sweet
Melon (CucumismeloL.), Cucumber (CucumissativusL.), Wormwood (Artemisiaargyi), tomato (Lycopersiconesculen-
tum Mil.), pepper (Capsicumannuum L.), cabbage [Brassicapekinensis (Lour.) Rupr.], longan (Dimocarpus
LonganLour.), litchi (Litchichinensis Sonn.), kiwi (ActinidiaChinensis) original variety identification, substantial faction
Raw variety identification and variety authenticity identification. The identification of other plant varieties can refer to this standard.
2 Normative references
The following documents are essential for the application of this document. For dated references, only the dated version applies to this article
Pieces. For the cited documents without date, the latest version (including all amendments) applies to this document.
GB/T 3543.2 Regulations for inspection of crop seeds
GB/T 6882 Acoustic sound pressure method for determination of noise source sound power level and sound energy level Precision methods for anechoic and semi-anechoic rooms
GB/T 19557.1 General guidelines for testing specificity, consistency and stability of new plant varieties
NY/T 2594 General rules of DNA molecular markers for plant variety identification
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Polynucleotide polymorphism; MNP
Sequence polymorphism caused by multiple nucleotides at the genomic level.
3.2
Essentially derived variety; EDV
Derived from the original variety or re-derived from the substantial variety of the original variety, and the genotype or gene of the original variety is retained
The basic characteristics produced by the type combination, but there are obvious differences from the original varieties.
3.3
Degree of variability
Alien plants (atypical plants) account for the percentage of the total observed plants.
3.4
Average coverage multiple
The ratio of the number of sequenced fragments to the number of labeled sites aligned to the labeled sites.
3.5
Detected markers
At least one allelic type has 20 or more marker sites supported by sequencing fragments.
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