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GB/T 38169-2019: Determination of caulerpin content - High performance liquid chromatography
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 38169-2019 | English | 119 |
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Determination of caulerpin content - High performance liquid chromatography
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GB/T 38169-2019
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Basic data | Standard ID | GB/T 38169-2019 (GB/T38169-2019) | | Description (Translated English) | Determination of caulerpin content - High performance liquid chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A21 | | Classification of International Standard | 07.080 | | Word Count Estimation | 6,692 | | Date of Issue | 2019-10-18 | | Date of Implementation | 2019-10-18 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 38169-2019: Determination of caulerpin content - High performance liquid chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of caulerpin content--High performance liquid chromatography
ICS 07.080
A21
National Standards of People's Republic of China
Determination of phycoerythrin content by high performance liquid chromatography
Published on.2019-10-18
2019-10-18 implementation
State market supervision and administration
China National Standardization Administration issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed and managed by the China National Institute of Standardization.
This standard was drafted. China Academy of Fishery Sciences, China Fisheries Research Institute, South China Sea Fisheries Research Institute, China National Institute of Standardization,
Beijing Sambo Technology Co., Ltd., China Rural Technology Development Center, Institute of Process Engineering, Chinese Academy of Sciences.
The main drafters of this standard. Yang Xianqing, Deng Jianchao, Li Le, Rong Hui, Song Jinlong, Qi Jianwei, Ma Haixia, Li Laihao, Ma Aijin, Hao Shuai,
Dai Bingye and Huang Yongdong.
Determination of phycoerythrin content by high performance liquid chromatography
1 Scope
This standard specifies methods for the determination of phycoerythrin by high performance liquid chromatography.
This standard applies to the determination of phycoerythrin content in natural plants of the fern.
The limit of quantitation of this method is 12.5 mg/kg.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 30891 Aquatic product sampling specification
3 Principle
The ethanol solution was added to the sample, and it was determined by microwave-assisted extraction, high performance liquid chromatography-ultraviolet detector, and external standard method.
4 reagents or materials
Analytically pure reagents are used unless otherwise specified.
4.1 Water. GB/T 6682, level one.
4.2 Anhydrous ethanol (CH3CH2OH). chromatographically pure.
4.3 Methanol (CH3OH). chromatographically pure.
4.4 85% (volume fraction) ethanol solution.
Take 850 mL of absolute ethanol, add 150 mL of water, and mix.
4.5 Brassin. purity ≥ 98%.
4.6 Standard stock solution of phycoerythrin.
Weigh 10.0mg of phycoerythrin, dissolve it in absolute ethanol, transfer it to a 100mL volumetric flask, dilute to the mark, mix and mix to make a concentration
Store at 100 μg/mL and store at -18 ° C for a period of 3 months.
4.7 Organic phase microporous membrane. pore size 0.22μm.
5 instruments
5.1 High Performance Liquid Chromatograph. equipped with a diode array detector or UV detector.
5.2 Atmospheric pressure microwave assisted extraction instrument. equipped with 100mL extraction tank.
5.3 Analytical balance. Sensitivity 0.01g, 0.0001g.
5.4 Buchner funnel.
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