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GB/T 36942-2018 English PDF

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GB/T 36942-2018: Determination of 10 alkaloids in cosmetics -- Liquid chromatography-tandem mass spectrometry
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PDF similar to GB/T 36942-2018


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Basic data

Standard ID GB/T 36942-2018 (GB/T36942-2018)
Description (Translated English) Determination of 10 alkaloids in cosmetics -- Liquid chromatography-tandem mass spectrometry
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard Y42
Classification of International Standard 71.100.70
Word Count Estimation 10,174
Date of Issue 2018-12-28
Date of Implementation 2019-07-01
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 36942-2018: Determination of 10 alkaloids in cosmetics -- Liquid chromatography-tandem mass spectrometry


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Determination of 10 alkaloids in cosmetics--Liquid chromatography--tandem mass spectrometry ICS 71.100.70 Y42 National Standards of People's Republic of China Determination of 10 alkaloids in cosmetics Liquid chromatography tandem mass spectrometry Published on.2018-12-28 2019-07-01 implementation State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is under the jurisdiction of the National Spice and Fragrance Cosmetics Standardization Technical Committee (SAC/TC257). This standard was drafted. Hebei Food Inspection Institute (National Fruit and Agricultural Processing Products Quality Supervision and Inspection Center), Suzhou Genealogy Testing Technology Co., Ltd., Hebei Entry-Exit Inspection and Quarantine Bureau, Hebei Medical University. The main drafters of this standard. Fan Sufang, Li Qiang, Zhang Yan, Ma Junmei, Yu Hao, Ai Lianfeng, Lu Pin, Ma Chunling, Wen Di, Ye Zhuhong, Dai Dan, 翟 Hong stability.

Introduction

The tested substances in this standard are metobase, saponin, aconitine, colchicine, atropine, and strychnine. Prohibited substances specified in the Code of Practice (2015 Edition). A banned substance is a substance that cannot be added to a cosmetic as a raw material for cosmetic production. Cosmetics Safety Technical Specifications (2015 edition) stipulates. If it is technically impossible to avoid the banned substances as impurities into the cosmetics, a safety risk assessment should be carried out to ensure Under normal, reasonable and foreseeable conditions of use, no harm may be caused to human health. At present, China has not specified the limit value of these substances. The formulation of this standard only provides testing methods for the determination of these substances in cosmetics. Determination of 10 alkaloids in cosmetics Liquid chromatography tandem mass spectrometry

1 Scope

This standard specifies the cosmetics of the Middle East, such as saponin, alkaloid, aconitine, hypaconitine, aconitine, colchicine, atropine, and Determination of the content of Ning, camptothecin and pilocarpine by liquid chromatography-tandem mass spectrometry (LC-MS/MS) Rate and precision, tolerance is poor. This standard applies to cream emulsion, water-based cosmetics, Middle East, alkaloids, aconitine, aconitine, aconitine, colchicum Determination of alkali, atropine, strychnine, camptothecin, pilocarpine content. The detection limit of this standard method is. atropine is 5μg/kg, hypoaconitine and neoaconitine are 7.5μg/kg, scopolamine, saponin, Aconitine, colchicine, strychnine, camptothecin, pilocarpine is 12.5μg/kg; the limit of quantification of this standard method. atropine is 12.5μg/kg, Secondary aconitine and aconitine were 25μg/kg, scopolamine, saponin, aconitine, colchicine, strychnine, camptothecin, pilocarpine 50 μg/kg.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Test methods

3.1 Principle The sample was ultrasonically extracted and centrifuged, and then determined and confirmed by liquid chromatography tandem mass spectrometry and quantified by external standard method. 3.2 Reagents and materials Unless otherwise stated, all reagents were of analytical grade and water was the primary water specified in GB/T 6682. 3.2.1 Methanol. Chromatographically pure. 3.2.2 70% aqueous methanol. 70mL methanol (3.2.1), 30mL water, mix and set aside. 3.2.3 Acetonitrile. Chromatographically pure. 3.2.4 n-hexane. chromatographically pure. 3.2.5 Formic acid. chromatographically pure. 3.2.6 0.1% formic acid aqueous solution. Accurately transfer formic acid (3.2.5) 1.0mL into a 1L volumetric flask and dilute to the mark with water. 3.2.7 Reference materials. scopolamine, saponin, aconitine, hypaconitine, neoaconitine, colchicine, atropine, strychnine, hi tree The Chinese name, English name, CAS number, molecular formula, relative molecular mass and structural formula of alkali and pilocarpine are shown in Table A.1 in Appendix A, pure The degree is not less than 96%. 3.2.8 Mixing standard stock solution. Accurately weigh the appropriate amount of each standard substance (3.2.7) into a 50mL volumetric flask and dilute to the volume with methanol.

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