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GB/T 36194-2018: Detection method of goldfish haematopoietic necrosis virus
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 36194-2018 | English | 179 |
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Detection method of goldfish haematopoietic necrosis virus
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GB/T 36194-2018
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Basic data | Standard ID | GB/T 36194-2018 (GB/T36194-2018) | | Description (Translated English) | Detection method of goldfish haematopoietic necrosis virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 65.020.30 | | Word Count Estimation | 9,954 | | Date of Issue | 2018-05-14 | | Date of Implementation | 2018-12-01 | | Regulation (derived from) | National Standards Announcement No. 6 of 2018 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 36194-2018: Detection method of goldfish haematopoietic necrosis virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Goldfish hematopoietic organ necrosis virus detection method)
ICS 65.020.30
B41
National Standards of People's Republic of China
Goldfish hematopoietic organ necrosis virus detection method
Published on.2018-05-14
2018-12-01 implementation
State market supervision and administration
China National Standardization Administration issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. Publication of this document
The organization is not responsible for identifying these patents.
This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic
This standard is under the jurisdiction of the National Aquaculture Standardization Technical Committee (SAC/TC156).
This standard was drafted. National Aquatic Technology Extension Station, China Academy of Inspection and Quarantine, Beijing Entry-Exit Inspection and Quarantine Bureau.
The main drafters of this standard. Li Qing, Wang Na, Gu Qiang, Zhang Wei, Jing Hongli, Wu Shaoqiang, Zhang Lifeng, Jiang Yulin, Chen Haonan.
Goldfish hematopoietic organ necrosis virus detection method
1 Scope
This standard specifies the PCR detection, fluorescent PCR detection and comprehensive determination of goldfish hematopoietic necrosis virus (herpesvirus type 2).
method.
This standard applies to the identification of goldfish hematopoietic necrosis virus, and is used in the epidemic of related diseases caused by goldfish hematopoietic necrosis virus.
Disease investigation, diagnosis, quarantine and monitoring.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 18088 entry and exit animal quarantine sampling
SC/T 7103 Technical specification for quarantine sampling of aquatic animals
3 Abbreviations
The following abbreviations apply to this document.
CyHV. herpes simplex virus (cyprinidherpesvirus)
CyHV-2. herpes simplex virus type 2 (cyprinidherpesvirus2)
GFHNV. goldfish haematopoieticnecrosisvirus
dNTP. deoxy-ribonucleoside triphosphate
Bp. basepair
Ct. cycle threshold (cyclethreshold)
CTAB. cetyltrimethylammonium bromide
EDTA. ethylenediaminetetraacetic acid
Tris. tris(hydroxymethyl)aminomethane [tris(hydroxymethyl)aminomethane]
4 reagents and materials
Analytically pure reagents were used unless otherwise stated.
4.1 Water. It should meet the specifications of the first grade water in GB/T 6682.
4.2 Taq DNA polymerase. 5 U/μL. Store at -20 °C to avoid repeated freezing and thawing or drastic changes in temperature.
4.3 dNTP. Contains dMTP, dGTP, dATP, dTTP each 10mmoL/L, stored at -20 °C.
4.4 Primers. The concentration was 10 μmol/L and stored at -20 °C.
a) CyHV primer
1) CyHVpol-F. 5'-CCCAGCAACATGTGCGACGG-3'
2) CyHVpol-R. 5'-CCGTARTGAGAGTTGGCGCA-3'
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