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GB/T 35951-2018 English PDF

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GB/T 35951-2018: Determination of eight macrolide antibiotics such as spiramycin in cosmetics -- LC-MS/MS
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PDF similar to GB/T 35951-2018


Standard similar to GB/T 35951-2018

GB/T 35953   GB/T 35949   GB/T 35950   GB/T 35948   GB/T 35946   

Basic data

Standard ID GB/T 35951-2018 (GB/T35951-2018)
Description (Translated English) Determination of eight macrolide antibiotics such as spiramycin in cosmetics -- LC-MS/MS
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard Y42
Classification of International Standard 71.100.70
Word Count Estimation 14,146
Date of Issue 2018-02-06
Date of Implementation 2018-09-01
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 35951-2018: Determination of eight macrolide antibiotics such as spiramycin in cosmetics -- LC-MS/MS


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of eight macrolide antibiotics such asspiramycin in cosmetics - LC-MS/MS ICS 71.100.70 Y42 National Standards of People's Republic of China Cosmetics Spiramycin and other 8 macrolides Antibiotics - Liquid chromatography - tandem mass spectrometry 2018-02-06 released 2018-09-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard proposed by the China Light Industry Federation. This standard by the National Spice cosmetics Standardization Technical Committee (SAC/TC257) centralized. This standard was drafted. Suzhou World Spectrum Detection Technology Co., Ltd., Guangdong Huaxin Detection Technology Co., Ltd., Jiangsu Long Li Qi Biotechnology Co., Ltd., Shanghai Daily Chemical Industry Institute, Perfect (China) Co., Ltd., European poetry Man Biological Co., Ltd., Suzhou quality Inspection and Scientific Research Institute, Hebei Province Institute of Food Inspection, Food and Drug Supervision and Research Institute of Jiangsu Province, Jiangsu Province, product quality supervision and inspection Research Institute, Suzhou University, Suzhou Division Technology Co., Ltd. The main drafters of this standard. Che Wenjun, Yang Yang, Shen Min, Dai Dan, Zhou Jianqing, Zhang Lihua, Li Xiaomin, Lu Xiaoyu, Yang security, Fan Sufang, Fang Ping, Yuan Liwen, Liu Yuan, Li Hai, Liao Huayong, Kang Wei, Wang Xiaodan, Zhang Zheng, Zhang Yan, Wu Zhongping, Lu Jian, Li Jianxiang, Huang Xueying.

Introduction

The standard test substance is China's "Cosmetics Safety Technical Specification (2015 Edition)" Prohibited substances. Prohibited substances are those that can not be added to cosmetics as a raw material for the production of cosmetics. "Cosmetic safety specification (2015 Edition) states. "If technically unavoidable banned substances are introduced as impurities into cosmetics, a safety risk assessment should be carried out to ensure Under normal, reasonable and predictable conditions of use shall not cause harm to human health. At present, China has not provided for the limited value of these substances, the development of this standard, only for the detection of these substances in cosmetics to provide testing methods. Cosmetics Spiramycin and other 8 macrolides Antibiotics - Liquid chromatography - tandem mass spectrometry

1 Scope

This standard specifies the cosmetics spiramycin, azithromycin, tilmicosin, oleandomycin, erythromycin, tylosin, clarithromycin, Luo Methods for the determination of erythromycin content by liquid chromatography-tandem mass spectrometry (LC-MS/MS), calculation of results, recovery and precision, Poor content. This standard applies to cream lotion, cosmetics, spirithromycin, azithromycin, tilmicosin, oleandomycin, erythromycin, Tylosin Su, clarithromycin, roxithromycin content determination. The detection limits and quantitative limits of this standard method, see Appendix A in Table A.1.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods

3 test methods

3.1 principle Samples were extracted by ultrasonic extraction, centrifugation, purification by solid phase extraction cartridges, liquid chromatography - tandem mass spectrometry determination and confirmation, external standard Legal quantity. 3.2 Reagents and materials Unless otherwise indicated, all reagents are of analytical grade, water is a water GB/T 6682 regulations. 3.2.1 methanol. chromatography pure. 3.2.2 Acetonitrile. Chromatographic purity. 3.2.3 Formic acid. Chromatographic purity. 3.2.4 n-hexane. chromatographic purity. 3.2.5 Sodium hydroxide. 3.2.6 sodium dihydrogen phosphate. 3.2.7 0.1mol/L sodium hydroxide solution. Weigh 4.0g sodium hydroxide, and diluted with water to 1L. 3.2.8 aqueous methanol solution (37). Measure 30mL of methanol (3.2.1) was mixed with 70mL of water. 3.2.9 Acetonitrile solution (28). Take 20 mL of acetonitrile (3.2.2) and mix with 80 mL of water. 3.2.10 phosphate buffer solution (0.1mol/L). Dissolved 12.2g sodium dihydrogen phosphate in 950mL water, with 0.1mol/L sodium hydroxide The solution was adjusted to pH 8.0 and finally diluted to 1 L with water. 3.2.11 8 kinds of macrolide antibiotics standard samples/substances. purity of not less than 96%. Spiramycin, azithromycin, tilmicosin, bamboo Amoxicillin, Erythromycin, Tylosin, Clarithromycin, Roxithromycin Drug Name, English Name, CAS Registry Number, Formula, Relative Molecular

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