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GB/T 35912-2018: Real-time RT-PCR method for detection of porcine reproductive and respiratory syndrome virus
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 35912-2018 | English | 199 |
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Real-time RT-PCR method for detection of porcine reproductive and respiratory syndrome virus
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GB/T 35912-2018
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Basic data | Standard ID | GB/T 35912-2018 (GB/T35912-2018) | | Description (Translated English) | Real-time RT-PCR method for detection of porcine reproductive and respiratory syndrome virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 10,185 | | Date of Issue | 2018-02-06 | | Date of Implementation | 2018-09-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 35912-2018: Real-time RT-PCR method for detection of porcine reproductive and respiratory syndrome virus
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Real-time RT-PCR method for detection of porcine reproductive and respiratory syndrome virus
ICS 11.220
B41
National Standards of People's Republic of China
Porcine reproductive and respiratory syndrome virus
Fluorescent RT-PCR detection method
Published on.2018-02-06
2018-09-01 Implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed by the Ministry of Agriculture of the People's
This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181).
This standard was drafted by. Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shanghai Institute of Veterinary Medicine, Chinese Academy of Agricultural Sciences, China
People's Republic of China Shenzhen Entry-Exit Inspection and Quarantine Bureau, Hunan Shengxiang Bio-Technology Co., Ltd., and China Academy of Inspection and Quarantine.
The main drafters of this standard. Zhang Qiang, Li Jian, Xiong Wei, Tong Guangzhi, Zhao Heping, Li Shuqing, Hua Qunyi, Yang Zhongping, Li Guoxin, Wang Qiaoquan,
Wang Yan, Lin Yingkai, Cai Kaimei, Yu Zhengjun, Lin Xiangmei, and Wu Shaoqiang.
Porcine reproductive and respiratory syndrome virus
Fluorescent RT-PCR detection method
1 Scope
This standard specifies the operation method for the detection of porcine reproductive and respiratory syndrome virus fluorescence RT-PCR.
This standard applies to the nucleic acid detection of the porcine reproductive and respiratory syndrome virus American strain.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB/T 6682 Analysis Laboratory Water Specifications and Test Methods
GB/T 25172 Technical Specification for Production and Preservation of Semen at Normal Temperature in Pigs
3 Abbreviations
The following abbreviations apply to this document.
Real-time RT-PCR. Real-time reverse transcription polymerase chainre-
Action)
Ct value. the number of cycles experienced when the amount of fluorescence signal in each reaction tube reaches the set threshold value (cyclethreshold)
RNA. ribonucleic acid
Taq enzyme. Taq DNA polymerase
TE buffer. Tris-EDTA buffer
DEPC. diethylpyrocarbonate
PRRSV. Porcine reproductive and respiratory syndrome virus (porcinereproductiveandrespiratorysyndromevirus)
PBS. phosphate buffered solution
4 Instruments
4.1 Fluorescent PCR detector.
4.2 High-speed desktop refrigerated centrifuge. Controllable temperature up to 4 °C, centrifugal speed up to 12000r/min or more.
4.3 Tissue grinder or mortar.
4.4 ordinary refrigerator. 2 °C ~ 8 °C.
4.5 ultra-low temperature refrigerator. Controllable temperature to -70 °C below.
4.6 Micropipettes. 0.2μL~2μL, 1μL~10μL, 10μL~100μL, 20μL~200μL, 100μL~1000μL, and
Equipped with a pipette to match the tip.
4.7 Autoclave.
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