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GB/T 35519-2017 English PDF

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GB/T 35519-2017: Chemicals -- Stably transfected human estrogen receptor transcriptional activation assay -- Detection of estrogenic agonists activation
Status: Valid

Standard ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status	PDF
GB/T 35519-2017	English	359	Add to Cart	4 days [Need to translate]	Chemicals -- Stably transfected human estrogen receptor transcriptional activation assay -- Detection of estrogenic agonists activation	Valid	GB/T 35519-2017

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Basic data

Standard ID	GB/T 35519-2017 (GB/T35519-2017)
Description (Translated English)	Chemicals -- Stably transfected human estrogen receptor transcriptional activation assay -- Detection of estrogenic agonists activation
Sector / Industry	National Standard (Recommended)
Classification of Chinese Standard	A80
Classification of International Standard	13.300; 11.100
Word Count Estimation	18,137
Date of Issue	2017-12-29
Date of Implementation	2018-07-01
Regulation (derived from)	National Standards Bulletin 2017 No. 32
Issuing agency(ies)	General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 35519-2017: Chemicals -- Stably transfected human estrogen receptor transcriptional activation assay -- Detection of estrogenic agonists activation

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Chemicals - Stably transfected human estrogen receptor transcriptional activation assay - Detection of estrogenic agonists activation ICS 13.300; 11.100 A80 National Standards of People's Republic of China Chemical stable transfection of human estrogen receptor Transcriptional activity test Estrogen agonist activity assay 2017-12-29 Posted 2018-07-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard and the Organization for Economic Co-operation and Development (OECD) Chemical Test Method No. 455 "Performance-BasedTest nists "(2012) technical content. This standard by the National Standardization Technical Committee of hazardous chemicals management (SAC/TC251) and focal point. This standard was drafted by Shanghai Exit and Entry Inspection and Quarantine of People's Republic of China, Chinese Academy of Inspection and Quarantine, Fudan University. The main drafters of this standard. Chen Xiang, Miao Wenbin, Chen Qing, Chen Huiming, Zhang Jing, Zhu Hongkun, Zhao Yiqing, Jiang Wei, Yao Lifang, Qingjiang, Wu Chunhua.

Introduction

This standard describes the use of stable transfected cell lines detect estrogen receptor agonist in vitro transcriptional activation test method, including the principle and Functionally similar estrogen receptor (ERs) agonists are designed to promote similar or improved detection methods to match Organization for Economic Co-operation and Development Guidance on Validation and Recognition of New Methods of Hazard Assessment. This standard Appendix A and Appendix B have been verified. Appendix A utilizes hERα-HeLa-9903 cells stably transfected with human ERα Transcriptional Activation Assay to Test Estrogenic Activity of Chemicals, Appendix B to BG1Luc-4E2 Cells that Express Human ERa Estrogen receptor transcriptional activation test. This standard estrogen receptor transcriptional activation test method can be used across countries, is conducive to data Mutual recognition. In.1998, the Organization for Economic Co-operation and Development initiated a priority project activity aimed at revising the current Guiding Principles, Potential new potential guidelines for endocrine disruptors. OECD measurements to assess potential endocrine disruptors The conceptual framework was revised in.2012. The conceptual framework consists of five phases, with different phases corresponding to different phases of biological complexity. This standard is The second phase of the "In Vitro Assay, Providing Endocrine Mechanisms/Access Information." This standard is to identify estrogen receptor agonist in vitro transcription Activate the test method. Chemical stable transfection of human estrogen receptor Transcriptional activity test Estrogen agonist activity assay

1 Scope

This standard specifies the use of stable transfected cell lines test estrogen receptor agonist terms and definitions, test principle, test side Law and Procedure, Data and Reporting. This standard applies to the use of stable transfected cell lines test estrogen receptor agonist assessment of chemical estrogen-like organisms active. 2 Terms, definitions and abbreviations 2.1 Terms and definitions The following terms and definitions apply to this document. 2.1.1 Agonist agonist A substance that produces a response (eg, transcription) when bound to a particular receptor. 2.1.2 Antagonist antagonist Ligands or ligands of a class of receptors that, upon binding to a receptor, do not themselves elicit a biological response, but block or inhibit agonist-mediated responses Learning products. 2.1.3 Anti-estrogenic activity anti-estrogenic activity A chemical that inhibits 17β-estradiol-mediated estrogen receptor activity. 2.1.4 Activated charcoal/dextran treatment charcoal/dextrantreatment Plasma treatment in cell culture removes endogenous hormones and hormone-binding proteins. 2.1.5 Cytotoxicity Toxic effects on cell structure or function and eventual cell death, compared to parallel solvent controls, are manifested as toxicity After exposure, the number of cells decreases or the cell function decreases. 2.1.6 Estrogenic activity Chemicals mimic 17β-estradiol's ability to bind and activate estrogen receptors. 2.1.7 Performance standard performancestandard Evaluate whether a test method is consistent with the verification method in terms of mechanism and function. include. --- Basic test methods; --- In the verification method used to prove that the test method is feasible list of the minimum control chemicals;

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